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Publikationer (10 of 59) Visa alla publikationer
Hruzova, K., Patel, A., Masák, J., Maťátková, O., Rova, U., Christakopoulos, P. & Matsakas, L. (2020). A novel approach for the production of green biosurfactant from Pseudomonas aeruginosa using renewable forest biomass. Science of the Total Environment, 711, Article ID 135099.
Öppna denna publikation i ny flik eller fönster >>A novel approach for the production of green biosurfactant from Pseudomonas aeruginosa using renewable forest biomass
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2020 (Engelska)Ingår i: Science of the Total Environment, ISSN 0048-9697, E-ISSN 1879-1026, Vol. 711, artikel-id 135099Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The rising demand for surfactants by the pharmaceuticals and cosmetic industries has generated vast amounts of petroleum-based synthetic surfactants, which are often toxic and non-degradable. Owing to their low toxicity, stability in extreme conditions, and biodegradability, biosurfactants could represent a sustainable alternative. The present study aimed to maximize the production of rhamnolipids (RL) from Pseudomonas aeruginosa by optimizing glucose concentration, temperature, and C/N and C/P ratios. After 96 h of cultivation at 37 °C, the final RL concentration was 4.18 ± 0.19 g/L with a final yield of 0.214 ± 0.010 g/gglucose when pure glucose was used as a carbon source. At present, the main obstacle towards commercialization of RL production is economic sustainability, due to the high cost of downstream processes and media components. For this reason, a renewable source such as wood hydrolysates (from birch and spruce woodchips) was examined here as a possible source of glucose for RL production. Both hydrolysates proved to be adequate, resulting in 2.34 ± 0.17 and 2.31 ± 0.10 g/L of RL, respectively, and corresponding yields of 0.081 ± 0.006 and 0.089 ± 0.004 g/gsugar after 96 h. These results demonstrate the potential of using renewable biomass for the production of biosurfactants and, to the best of our knowledge, they constitute the first report on the use of wood hydrolysates for RL production.

Ort, förlag, år, upplaga, sidor
Elsevier, 2020
Nyckelord
Rhamnolipid, Biosurfactants, Pseudomonas, Wood hydrolysate, Organosolv fractionation
Nationell ämneskategori
Bioprocessteknik
Forskningsämne
Biokemisk processteknik
Identifikatorer
urn:nbn:se:ltu:diva-76888 (URN)10.1016/j.scitotenv.2019.135099 (DOI)000509344700013 ()2-s2.0-85076241604 (Scopus ID)
Anmärkning

Validerad;2020;Nivå 2;2020-02-25 (johcin)

Tillgänglig från: 2019-11-27 Skapad: 2019-11-27 Senast uppdaterad: 2020-03-13Bibliografiskt granskad
Najjarzadeh, N., Krige, A., Pamidi, T. R., Johansson, Ö., Enman, J., Matsakas, L., . . . Christakopoulos, P. (2020). Numerical modeling and verification of a sonobioreactor and its application on two model microorganisms. PLoS ONE, 15(3), Article ID e0229738.
Öppna denna publikation i ny flik eller fönster >>Numerical modeling and verification of a sonobioreactor and its application on two model microorganisms
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2020 (Engelska)Ingår i: PLoS ONE, E-ISSN 1932-6203, Vol. 15, nr 3, artikel-id e0229738Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Ultrasound has many uses, such as in medical imaging, monitoring of crystallization, characterization of emulsions and suspensions, and disruption of cell membranes in the food industry. It can also affect microbial cells by promoting or slowing their growth and increasing the production of some metabolites. However, the exact mechanism explaining the effect of ultrasound has not been identified yet. Most equipment employed to study the effect of ultrasound on microorganisms has been designed for other applications and then only slightly modified. This results in limited control over ultrasound frequency and input power, or pressure distribution in the reactor. The present study aimed to obtain a well-defined reactor by simulating the pressure distribution of a sonobioreactor. Specifically, we optimized a sonotrode to match the bottle frequency and compared it to measured results to verify the accuracy of the simulation. The measured pressure distribution spectrum presented the same overall trend as the simulated spectrum. However, the peaks were much less intense, likely due to non-linear events such as the collapse of cavitation bubbles. To test the application of the sonobioreactor in biological systems, two biotechnologically interesting microorganisms were assessed: an electroactive bacterium, Geobacter sulfurreducens, and a lignocellulose-degrading fungus, Fusarium oxysporum. Sonication resulted in increased malate production by Gsulfurreducens, but no major effect on growth. In comparison, morphology and growth of Foxysporum were more sensitive to ultrasound intensity. Despite considerable morphological changes at 4 W input power, the growth rate was not adversely affected; however, at 12 W, growth was nearly halted. The above findings indicate that the novel sonobioreactor provides an effective tool for studying the impact of ultrasound on microorganisms.

Ort, förlag, år, upplaga, sidor
PLOS, 2020
Nationell ämneskategori
Bioprocessteknik Strömningsmekanik och akustik
Forskningsämne
Biokemisk processteknik; Teknisk akustik
Identifikatorer
urn:nbn:se:ltu:diva-78111 (URN)10.1371/journal.pone.0229738 (DOI)32160222 (PubMedID)2-s2.0-85081204531 (Scopus ID)
Anmärkning

Validerad;2020;Nivå 2;2020-03-26 (alebob)

Tillgänglig från: 2020-03-19 Skapad: 2020-03-19 Senast uppdaterad: 2020-03-26Bibliografiskt granskad
Hruzova, K., Matsakas, L., Sand, A., Rova, U. & Christakopoulos, P. (2020). Organosolv lignin hydrophobic micro- and nanoparticles as a low-carbon footprint biodegradable flotation collector in mineral flotation. Bioresource Technology, Article ID 123235.
Öppna denna publikation i ny flik eller fönster >>Organosolv lignin hydrophobic micro- and nanoparticles as a low-carbon footprint biodegradable flotation collector in mineral flotation
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2020 (Engelska)Ingår i: Bioresource Technology, ISSN 0960-8524, E-ISSN 1873-2976, artikel-id 123235Artikel i tidskrift (Refereegranskat) In press
Abstract [en]

Flotation is a key step during mineral separation. Xanthates are the most commonly used collectors for recovering Cu, Ni, and Zn from sulphide ores. However, xanthates are fossil-based and toxic for the environment. The aim of this study was to evaluate the use of lignin nanoparticles and microparticles as sustainable and environmentally friendly collectors. Lignin particles demonstrated good selectivity toward Cu (chalcopyrite), with total recoveries exceeding 80% and grades of up to 8.6% w/w from a Cu-Ni ore in rougher flotation tests. When floating Zn-Pb-Cu ore, lignin nanoparticles could reduce the use of xanthates by 50%. Moreover, they outperformed xanthates alone, achieving total recoveries of up to 91%, 85%, and 98% for Cu, Pb, and Zn, respectively. These results prove the potential of lignin as a flotation collector.

Ort, förlag, år, upplaga, sidor
Elsevier, 2020
Nyckelord
Lignin, Nanoparticles, Flotation, Collector, Organosolv
Nationell ämneskategori
Bioprocessteknik
Forskningsämne
Biokemisk processteknik
Identifikatorer
urn:nbn:se:ltu:diva-78359 (URN)10.1016/j.biortech.2020.123235 (DOI)2-s2.0-85082530221 (Scopus ID)
Tillgänglig från: 2020-04-06 Skapad: 2020-04-06 Senast uppdaterad: 2020-04-06
Matsakas, L., Gerber, M., Yu, L., Rova, U. & Christakopoulos, P. (2020). Preparation of low carbon impact lignin nanoparticles with controllable size by using different strategies for particles recovery. Industrial crops and products (Print), 147, Article ID 112243.
Öppna denna publikation i ny flik eller fönster >>Preparation of low carbon impact lignin nanoparticles with controllable size by using different strategies for particles recovery
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2020 (Engelska)Ingår i: Industrial crops and products (Print), ISSN 0926-6690, E-ISSN 1872-633X, Vol. 147, artikel-id 112243Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Lignin still remains an underutilized plentiful resource whose conversion to high-added value products is a cornerstone towards establishing a viable biomass biorefinery. Bio-materials in the form of nanoparticles represent promising high-value products with numerous downstream applications. The aim of the current work was to develop a method that would allow controlling the size of (birch and spruce) lignin nano- and micro-particles for their subsequent recovery into a solid product. We tested different two-step and one-step isolation processes and demonstrated that particle size could be easily controlled to meet different ranges (<100 nm, <500 nm, and>1 μm). In general, two-step isolation methods, i.e. a step of decrease of solvent concentration followed by isolation of lignin particles, were better for the isolation of well-defined spherical particles. In particular, the rate at which ethanol concentration was decreased played a significant role in determining the size of lignin particles. Moreover, when lignin concentration was increased from 1 % to 5 % and 10 % (w/v), particle size and homogeneity decreased slightly, but productivity augmented. The present study demonstrates that different isolation methods can be applied to obtain renewable, customarily sized, lignin spherical micro- and nano-particles.

Ort, förlag, år, upplaga, sidor
Elsevier, 2020
Nyckelord
Lignin, Nanoparticles, Organosolv, Birch, Spruce
Nationell ämneskategori
Bioprocessteknik Kemiska processer
Forskningsämne
Biokemisk processteknik; Kemisk teknologi
Identifikatorer
urn:nbn:se:ltu:diva-77832 (URN)10.1016/j.indcrop.2020.112243 (DOI)
Anmärkning

Validerad;2020;Nivå 2;2020-02-24 (alebob)

Tillgänglig från: 2020-02-24 Skapad: 2020-02-24 Senast uppdaterad: 2020-02-24Bibliografiskt granskad
Paulsen Thoresen, P., Matsakas, L., Rova, U. & Christakopoulos, P. (2020). Recent advances in organosolv fractionation: Towards biomass fractionation technology of the future. Bioresource Technology, Article ID 123189.
Öppna denna publikation i ny flik eller fönster >>Recent advances in organosolv fractionation: Towards biomass fractionation technology of the future
2020 (Engelska)Ingår i: Bioresource Technology, ISSN 0960-8524, E-ISSN 1873-2976, artikel-id 123189Artikel i tidskrift (Refereegranskat) In press
Abstract [en]

Organosolv treatment is among the most promising strategies for valorising lignocellulosic biomass and could facilitate the transition towards enhanced utilization of renewable feedstocks. However, issues such as inefficient solvent recycle and fractionation has to be overcome. The present review aims to address these issues and discuss the role of the components present during organosolv treatment and their influence on the overall process. Thus, the review focuses not only on how the choice of solvent and catalyst affects lignocellulosic fractionation, but also on how the choice of treatment liquor influences the possibility for solvent recycling and product isolation. Several organic solvents have been investigated in combination with water and acid/base catalysts; however, the lack of a holistic approach often compromises the performance of the different operational units. Thus, an economically viable organosolv process should optimize biomass fractionation, product isolation, and solvent recycling.

Ort, förlag, år, upplaga, sidor
Elsevier, 2020
Nyckelord
Biomass, Lignocellulose, Organosolv, Ionic liquids, Biorefinery
Nationell ämneskategori
Bioprocessteknik
Forskningsämne
Biokemisk processteknik
Identifikatorer
urn:nbn:se:ltu:diva-78374 (URN)10.1016/j.biortech.2020.123189 (DOI)32220471 (PubMedID)2-s2.0-85082462286 (Scopus ID)
Tillgänglig från: 2020-04-07 Skapad: 2020-04-07 Senast uppdaterad: 2020-04-07
Antonopoulou, I., Spanopoulos, A. & Matsakas, L. (2020). Single cell oil and ethanol production by the oleaginous yeast Trichosporon fermentans utilizing dried sweet sorghum stalks. Renewable energy, 146, 1609-1617
Öppna denna publikation i ny flik eller fönster >>Single cell oil and ethanol production by the oleaginous yeast Trichosporon fermentans utilizing dried sweet sorghum stalks
2020 (Engelska)Ingår i: Renewable energy, ISSN 0960-1481, E-ISSN 1879-0682, Vol. 146, s. 1609-1617Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The ability of the oleaginous yeast Trichosporon fermentans to efficiently produce lipids when cultivated in dried sweet sorghum was evaluated. First, lipid production was evaluated in synthetic media mimicking the composition of sweet sorghum stalks and optimized based on the nitrogen source and C: N ratio. Under optimum conditions, the lipid production reached 3.66 g/L with 21.91% w/w lipid content by using a mixture of sucrose, glucose and fructose and peptone at C: N ratio 160. Cultivation on pre-saccharified sweet sorghum stalks offered 1.97 g/L, while it was found that sweet sorghum stalks can support yeast growth and lipid production without the need for external nitrogen source addition. At an attempt to increase the carbon source concentration for optimizing lipid production, the Crabtree effect was observed in T. fermentans. To this end, the yeast was evaluated for its potential to produce ethanol under anaerobic conditions in synthetic media and sweet sorghum. The ethanol concentration at 100 g/L glucose was 40.31 g/L, while utilizing sweet sorghum by adding a distinct saccharification step and external nitrogen source offered ethanol concentration equal to 23.5 g/L. To the authors’ knowledge, this is the first time that the Crabtree effect is observed in T. fermentans.

Ort, förlag, år, upplaga, sidor
Elsevier, 2020
Nyckelord
Trichosporon fermentans CBS 439.83, Crabtree effect, Ethanol, Sweet sorghum, Enzymatic saccharification, Microbial lipids
Nationell ämneskategori
Kemiteknik Bioprocessteknik
Forskningsämne
Biokemisk processteknik
Identifikatorer
urn:nbn:se:ltu:diva-75377 (URN)10.1016/j.renene.2019.07.107 (DOI)000499762300016 ()2-s2.0-85069920649 (Scopus ID)
Anmärkning

Validerad;2019;Nivå 2;2019-08-14 (johcin)

Tillgänglig från: 2019-07-30 Skapad: 2019-07-30 Senast uppdaterad: 2019-12-20Bibliografiskt granskad
Patel, A. & Matsakas, L. (2019). A comparative study on de novo and ex novo lipid fermentation by oleaginous yeast using glucose and sonicated waste cooking oil. Ultrasonics sonochemistry, 52, 364-374
Öppna denna publikation i ny flik eller fönster >>A comparative study on de novo and ex novo lipid fermentation by oleaginous yeast using glucose and sonicated waste cooking oil
2019 (Engelska)Ingår i: Ultrasonics sonochemistry, ISSN 1350-4177, E-ISSN 1873-2828, Vol. 52, s. 364-374Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

There are only a few reports available about the assimilation of hydrophobic substrates by microorganisms, however, it is well known that oleaginous microorganisms are capable of utilizing both hydrophilic and hydrophobic substrates and accumulate lipids via two different pathways namely de novo and ex novo lipid synthesis, respectively. In the present study, an oleaginous yeast, Cryptococcus curvatus, was investigated for its potentials to utilize a waste substrate of hydrophobic nature (waste cooking oil – WCO) and compared with its ability to utilize a hydrophilic carbon source (glucose). To facilitate the utilization of WCO by C. curvatus, the broth was sonicated to form a stable oil-in-water emulsion without adding any emulsifier, which was then compared with WCO samples without any ultrasound treatment (unsonicated) for the yeast cultivation. Ultrasonication reduces the size of hydrophobic substrates and improves their miscibility in an aqueous broth making them easily assimilated by oleaginous yeast. Under de novo lipid fermentation, the yeast synthesized 9.93 ± 0.84 g/L of cell dry weight and 5.23 ± 0.49 g/L lipids (lipid content of 52.66 ± 0.93% w/w) when cultivated on 40 g/L of glucose (C/N ratio of 40). The amount of cell dry weight, lipid concentration, and lipid content were considerably higher during the ex novo lipid synthesis. More specifically, the highest lipid content achieved was 70.13 ± 1.65% w/w with a corresponding dry cell weight and lipid concentration of 18.62 ± 0.76 g/L and 13.06 ± 0.92 g/L respectively, when grown on 20 g/L sonicated WCO. The highest lipid concentration, however, was observed when the yeast was cultivated on 40 g/L sonicated WCO. Under these conditions, 20.34 g/L lipids were produced with a lipid content of 57.05% w/w. On the other hand, lipid production with unsonicated WCO was significant lower, reaching 11.16 ± 1.02 g/L (69.14 ± 1.34% w/w of lipid content) and 12.21 ± 1.34 g/L (47.39 ± 1.67% w/w of lipid content) for 20 g/L and 40 g/L of WCO, respectively. This underpins the significance of the sonication treatment, especially at elevated WCO concentrations, to improve the accessibility of the yeast to the WCO. Sonication treatment that was used in this study assisted the utilization of WCO without the need to add emulsifiers, thus reducing the need for chemicals and in turn has a positive impact on the production costs. The microbial lipids produced presented a different fatty acid composition compared to the WCO, making them more suitable for biodiesel production as suggested by the theoretical estimation of the biodiesel properties.

Ort, förlag, år, upplaga, sidor
Elsevier, 2019
Nyckelord
Oleaginous yeast, Sonicated waste cooking oil, De novo lipid accumulation, Ex novo lipid accumulation, LipidsFatty acid methyl esters
Nationell ämneskategori
Bioprocessteknik
Forskningsämne
Biokemisk processteknik
Identifikatorer
urn:nbn:se:ltu:diva-72671 (URN)10.1016/j.ultsonch.2018.12.010 (DOI)000467509200041 ()2-s2.0-85058415972 (Scopus ID)
Anmärkning

Validerad;2019;Nivå 2;2019-05-27 (oliekm)

Tillgänglig från: 2019-01-24 Skapad: 2019-01-24 Senast uppdaterad: 2019-08-19Bibliografiskt granskad
Patel, A., Matsakas, L., Rova, U. & Christakopoulos, P. (2019). A perspective on biotechnological applications of thermophilic microalgae and cyanobacteria. Bioresource Technology, 278, 424-434
Öppna denna publikation i ny flik eller fönster >>A perspective on biotechnological applications of thermophilic microalgae and cyanobacteria
2019 (Engelska)Ingår i: Bioresource Technology, ISSN 0960-8524, E-ISSN 1873-2976, Vol. 278, s. 424-434Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The importance of expanding our knowledge on microorganisms derived from extreme environments stems from the development of novel and sustainable technologies for our health, food, and environment. Microalgae and cyanobacteria represent a group of diverse microorganisms that inhabit a wide range of environments, are capable of oxygenic photosynthesis, and form a thick microbial mat even at extreme environments. Studies of thermophilic microorganisms have shown a considerable biotechnological potential due to their optimum growth and metabolisms at high temperatures (≥50 °C), which is supported by their thermostable enzymes. Microalgal and cyanobacterial communities present in high-temperature ecosystems account for a large part of the total ecosystem biomass and productivity, and can be exploited to generate several value-added products of agricultural, pharmaceutical, nutraceutical, and industrial relevance. This review provides an overview on the current status of biotechnological applications of thermophilic microalgae and cyanobacteria, with an outlook on the challenges and future prospects.

Ort, förlag, år, upplaga, sidor
Elsevier, 2019
Nyckelord
Thermophilic microorganisms, Microalgae, Cyanobacteria, Biotechnological applications, Biofuels, Biologically active compounds, Pigments
Nationell ämneskategori
Bioprocessteknik
Forskningsämne
Biokemisk processteknik
Identifikatorer
urn:nbn:se:ltu:diva-73043 (URN)10.1016/j.biortech.2019.01.063 (DOI)000457852400049 ()30685131 (PubMedID)2-s2.0-85060269950 (Scopus ID)
Anmärkning

Validerad;2019;Nivå 2;2019-02-27 (johcin)

Tillgänglig från: 2019-02-27 Skapad: 2019-02-27 Senast uppdaterad: 2019-02-27Bibliografiskt granskad
Kalogiannis, K. G., Matsakas, L., Lappas, A. A., Rova, U. & Christakopoulos, P. (2019). Aromatics from Beechwood Organosolv Lignin through Thermal and Catalytic Pyrolysis. Energies, 12(9), Article ID 1606.
Öppna denna publikation i ny flik eller fönster >>Aromatics from Beechwood Organosolv Lignin through Thermal and Catalytic Pyrolysis
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2019 (Engelska)Ingår i: Energies, ISSN 1996-1073, E-ISSN 1996-1073, Vol. 12, nr 9, artikel-id 1606Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Biomass fractionation, as an alternative to biomass pretreatment, has gained increasing research attention over the past few years as it provides separate streams of cellulose, hemicellulose, and lignin. These streams can be used separately and can provide a solution for improving the economics of emerging biorefinery technologies. The sugar streams are commonly used in microbial conversions, whereas during recent years lignin has been recognized as a valuable compound as it is the only renewable and abundant source of aromatic chemicals. Successfully converting lignin into valuable chemicals and products is key in achieving both environmental and economic sustainability of future biorefineries. In this work, lignin retrieved from beechwood sawdust delignification pretreatment via an organosolv process was depolymerized with thermal and catalytic pyrolysis. ZSM-5 commercial catalyst was used in situ to upgrade the lignin bio-oil vapors. Lignins retrieved from different modes of organosolv pretreatment were tested in order to evaluate the effect that upstream pretreatment has on the lignin fraction. Both thermal and catalytic pyrolysis yielded oils rich in phenols and aromatic hydrocarbons. Use of ZSM-5 catalyst assisted in overall deoxygenation of the bio-oils and enhanced aromatic hydrocarbons production. The oxygen content of the bio-oils was reduced at the expense of their yield. Organosolv lignins were successfully depolymerized towards phenols and aromatic hydrocarbons via thermal and catalytic pyrolysis. Hence, lignin pyrolysis can be an effective manner for lignin upgrading towards high added value products

Ort, förlag, år, upplaga, sidor
MDPI, 2019
Nyckelord
beechwood, organosolv delignification, lignin pyrolysis, ZSM-5, phenols, aromatic hydrocarbons
Nationell ämneskategori
Bioprocessteknik
Forskningsämne
Biokemisk processteknik
Identifikatorer
urn:nbn:se:ltu:diva-74900 (URN)10.3390/en12091606 (DOI)000469761700022 ()2-s2.0-85066273519 (Scopus ID)
Anmärkning

Validerad;2019;Nivå 2;2019-06-24 (johcin)

Tillgänglig från: 2019-06-24 Skapad: 2019-06-24 Senast uppdaterad: 2019-06-24Bibliografiskt granskad
Patel, A., Matsakas, L., Hruzova, K., Rova, U. & Christakopoulos, P. (2019). Biosynthesis of Nutraceutical Fatty Acids by the Oleaginous Marine Microalgae Phaeodactylum tricornutum Utilizing Hydrolysates from Organosolv-Pretreated Birch and Spruce Biomass. Marine Drugs, 17(12), Article ID 119.
Öppna denna publikation i ny flik eller fönster >>Biosynthesis of Nutraceutical Fatty Acids by the Oleaginous Marine Microalgae Phaeodactylum tricornutum Utilizing Hydrolysates from Organosolv-Pretreated Birch and Spruce Biomass
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2019 (Engelska)Ingår i: Marine Drugs, ISSN 1660-3397, E-ISSN 1660-3397, Vol. 17, nr 12, artikel-id 119Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Polyunsaturated fatty acids (PUFAs) are essential for human function, however they have to be provided through the diet. As their production from fish oil is environmentally unsustainable, there is demand for new sources of PUFAs. The aim of the present work was to establish the microalgal platform to produce nutraceutical-value PUFAs from forest biomass. To this end, the growth of Phaeodactylum tricornutum on birch and spruce hydrolysates was compared to autotrophic cultivation and glucose synthetic media. Total lipid generated by P. tricornutum grown mixotrophically on glucose, birch, and spruce hydrolysates was 1.21, 1.26, and 1.29 g/L, respectively. The highest eicosapentaenoic acid (EPA) production (256 mg/L) and productivity (19.69 mg/L/d) were observed on spruce hydrolysates. These values were considerably higher than those obtained from the cultivation without glucose (79.80 mg/L and 6.14 mg/L/d, respectively) and also from the photoautotrophic cultivation (26.86 mg/L and 2.44 mg/L/d, respectively). To the best of our knowledge, this is the first report describing the use of forest biomass as raw material for EPA and docosapentaenoic acid (DHA) production.

Ort, förlag, år, upplaga, sidor
MDPI, 2019
Nyckelord
polyunsaturated fatty acids, EPA, DHA, marine algae, Phaeodactylum tricornutum, forest biomass
Nationell ämneskategori
Bioprocessteknik
Forskningsämne
Biokemisk processteknik
Identifikatorer
urn:nbn:se:ltu:diva-73136 (URN)10.3390/md17020119 (DOI)000460795500047 ()30781416 (PubMedID)2-s2.0-85061857091 (Scopus ID)
Anmärkning

Validerad;2019;Nivå 2;2019-03-07 (johcin)

Tillgänglig från: 2019-03-07 Skapad: 2019-03-07 Senast uppdaterad: 2019-04-24Bibliografiskt granskad
Organisationer
Identifikatorer
ORCID-id: ORCID iD iconorcid.org/0000-0002-3687-6173

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