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Rodríguez, Nathalie PérezORCID iD iconorcid.org/0000-0002-9389-0289
Alternative names
Publications (10 of 11) Show all publications
Rodríguez, N. P., Khoshkhoo, M., Sandström, Å., Rodushkin, I., Alakangas, L. & Öhlander, B. (2015). Isotopic signature of Cu and Fe during bioleaching and electrochemical leaching of a chalcopyrite concentrate (ed.). Paper presented at . International Journal of Mineral Processing, 134, 58-65
Open this publication in new window or tab >>Isotopic signature of Cu and Fe during bioleaching and electrochemical leaching of a chalcopyrite concentrate
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2015 (English)In: International Journal of Mineral Processing, ISSN 0301-7516, E-ISSN 1879-3525, Vol. 134, p. 58-65Article in journal (Refereed) Published
Abstract [en]

Bioleaching is an important process in metallurgy and in environmental sciences, either for the acquisition of metals or for the formation of acid rock drainage. In this study the implications of the processes during bioleaching of a pyritic chalcopyrite concentrate were analysed regarding its Cu and Fe isotope fractionation. The development of the redox potential during the bioleaching experiment was then simulated in an electrochemical cell in absence of microorganisms to investigate the effect of microbial activity on the Cu and Fe isotope fractionations. The leaching experiments were performed for 28 days at 45 °C with a solid content of 2.5% (w/v) at pH 1.5. It was found that Cu dissolution efficiency was similar in both experiments and the leaching curves were linear with no sign of passivation due to presence of pyrite. The heavy Cu isotope (δ65Cu) was leached more easily and as a result the leachate was enriched with the heavy Cu isotope at the beginning of both experiments and as the leaching progressed δ65Cu values in the leachate became similar to the ones of the chalcopyrite concentrate, confirming an equilibrium fractionation happening in a closed system. There was no distinct difference in the Cu and Fe isotope fractionations in absence and presence of microorganisms. Finally based on Cu and Fe isotope signatures, a simplified method is suggested for the estimation of the leaching extent during the oxidisation of sulphide materials in natural systems.

National Category
Metallurgy and Metallic Materials Geochemistry
Research subject
Process Metallurgy; Applied Geology
Identifiers
urn:nbn:se:ltu:diva-5240 (URN)10.1016/j.minpro.2014.11.010 (DOI)000348960600008 ()2-s2.0-84924082946 (Scopus ID)348dc9fd-2b99-4601-aa07-7e40c905974c (Local ID)348dc9fd-2b99-4601-aa07-7e40c905974c (Archive number)348dc9fd-2b99-4601-aa07-7e40c905974c (OAI)
Note
Validerad; 2014; Nivå 2; 20141128 (andbra)Available from: 2016-09-29 Created: 2016-09-29 Last updated: 2019-11-22Bibliographically approved
Rodríguez, N. P., Langella, F., Rodushkin, I., Engström, E., Kothe, E., Alakangas, L. & Öhlander, B. (2014). The role of bacterial consortium and organic amendment in Cu and Fe isotope fractionation in plants on a polluted mine site (ed.). Paper presented at . Environmental science and pollution research international, 21(11), 6836-6844
Open this publication in new window or tab >>The role of bacterial consortium and organic amendment in Cu and Fe isotope fractionation in plants on a polluted mine site
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2014 (English)In: Environmental science and pollution research international, ISSN 0944-1344, E-ISSN 1614-7499, Vol. 21, no 11, p. 6836-6844Article in journal (Refereed) Published
Abstract [en]

Copper and iron isotope fractionation by plant uptake and translocation is a matter of current research. As a way to apply the use of Cu and Fe stable isotopes in the phytoremediation of contaminated sites, the effects of organic amendment and microbial addition in a mine spoiled soil seeded with Helianthus annuus in pot experiments and field trials were studied. Results show that the addition of a microbial consortium of ten bacterial strains has an influence on Cu and Fe isotope fractionation by the uptake and translocation in pot experiments, with an increase in average of 0.99‰ for the δ65Cu values from soil to roots. In the field trial, the amendment with the addition of bacteria and mycorrhiza as single and double inoculation enriches the leaves in 65Cu compared to the soil. As a result of the same trial, the δ56Fe values in the leaves are lower than those from the bulk soil, although some differences are seen according to the amendment used. Siderophores, possibly released by the bacterial consortium, can be responsible for this change in the Cu and Fe fractionation. The overall isotopic fractionation trend for Cu and Fe does not vary for pots and field experiments with or without bacteria. However variations in specific metabolic pathways related to metal-organic complexation and weathering can modify particular isotopic signatures.

Keywords
Cu isotopes, Fe isotopes, mine sites, phytoremediation, organic amendments, higher plants, PGPR (plant growth promoting bacteria), metal uptake, Earth sciences - Exogenous eart sciences, Geovetenskap - Exogen geovetenskap
National Category
Geochemistry
Research subject
Applied Geology
Identifiers
urn:nbn:se:ltu:diva-11594 (URN)10.1007/s11356-013-2156-1 (DOI)000336371000008 ()24057964 (PubMedID)2-s2.0-84901238232 (Scopus ID)a9995b28-bd8d-4e8d-8754-1ddb884bc4f4 (Local ID)a9995b28-bd8d-4e8d-8754-1ddb884bc4f4 (Archive number)a9995b28-bd8d-4e8d-8754-1ddb884bc4f4 (OAI)
Projects
Using MicroBes for the REgulation of heavy metaL mobiLity at ecosystem and landscape scAle: an integrative approach for soil remediation by geobiological processes
Note
Validerad; 2014; 20130910 (natper)Available from: 2016-09-29 Created: 2016-09-29 Last updated: 2019-11-22Bibliographically approved
Rodríguez, N. P. (2013). Biotic and abiotic isotope fractionation of copper and iron: From the lab to the field scale (ed.). (Doctoral dissertation). Paper presented at . : Luleå tekniska universitet
Open this publication in new window or tab >>Biotic and abiotic isotope fractionation of copper and iron: From the lab to the field scale
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The distribution of the stable isotopes of Cu and Fe in nature is susceptible to isotope fractionation processes during the biogeochemical cycle. Since Cu and Fe are redox sensitive metals, differences in their oxidation states can lead to variations in the stable isotope composition of the aquatic species or compounds that they form. Stable isotopes of Cu and Fe have recently been used to trace metal redox cycles, nutrient pathways, metal contaminant sources and to develop isotopic biosignatures. The objective of this project was to study the geochemical processes governing the isotopic fractionation of Cu and Fe in mine impacted sites, including processes related to mineral processing. One of the key questions was to explain the role of bacteria in the variations of the isotopic composition of Cu and Fe. First, bioleaching and electroleaching of a chalcopyrite concentrate were performed. During the chalcopyrite leaching in both experiments the first release of Cu to the leachate is enriched in the heavier Cu isotope as a product of oxidative dissolution. At the later stages of leaching, the δ65Cu values for the leachate are similar to the initial material, confirming an equilibrium fractionation in a closed system. In the case of Fe isotope fractionation the dissolution of pyrite at redox potentials higher than 600mV leads to an enrichment of the heavier Fe isotope in the leachate in the bioleaching experiment, mainly regulated by the formation of secondary minerals such as jarosite. Soil bacteria were studied in three different experimental scales using pot, lysimeters and field experiments, amended with autochthonous plant growth promoting bacteria. Roots and plants from pots showed no variation in their Fe and Cu isotope composition compared to non-amended samples. However, plants growing in the amended substrates regardless of their experimental scale, showed variations in the Fe and Cu isotope composition of their roots with an increase in the heavier Cu isotope. Siderophores released either by bacteria or the plant can complexate available Cu and Fe in the soil, causing a change in the isotope fractionation of those metals. The second question is related to the biogeochemical cycle of Cu and Fe. In mine tailings the sulphide oxidation resulted in an enrichment of the lighter Cu isotope in secondary phases in the oxidized zone of the tailings compared to the original isotope composition in the unoxidised mineral. Precipitation of covellite at the oxidation front of the tailings profile resulted in a significant enrichment of the lighter Cu isotope in the bulk soil with a δ65Cu value as low as -4.35 ±0.02 ‰. Fe isotope fractionation in the Kristineberg test cell varied due to processes such as Fe(II)-Fe(III) equilibrium and precipitation of Fe-(oxy)hydroxides at the oxidation front, where δ56Fe values were higher than in the initial material. As a way to link the obtained results from this thesis, a self-restored mine site was studied. A variation towards higher δ65Cu values was seen from rocks, to water and biofilms. Cu absorption mainly by extrapolymeric substances and secondary mineral precipitation regulates the isotopic composition of the biofilm. Oxidative weathering of sulphide minerals and further precipitation of Fe-(oxy)hydroxides are considered to be the main causes for Fe isotope fractionation in this area. Summing up, this thesis provides several field studies to corroborate the data observed in the lab regarding processes that are important for the biogeochemical cycling of metals and could be further applied to the extraction of metals or for remediation purposes.

Place, publisher, year, edition, pages
Luleå tekniska universitet, 2013. p. 46
National Category
Geochemistry
Research subject
Applied Geology
Identifiers
urn:nbn:se:ltu:diva-26743 (URN)fe44929a-a35f-467a-bccd-74420f437868 (Local ID)978-91-7439-793-2 (ISBN)978-91-7439-794-9 (ISBN)fe44929a-a35f-467a-bccd-74420f437868 (Archive number)fe44929a-a35f-467a-bccd-74420f437868 (OAI)
Note
Godkänd; 2013; 20131028 (natper); Dissertation to be held in public in room E632 on Tuesday 17th of December at 10:00 am. External examiner: Dr. Dominik Weiss, Department for Earth Sciences and Engineering, Imperial College London. Chairman: Professor Björn Öhlander, Division of Geosciences and Environmental Engineering, Luleå University of Technology. --- Tillkännagivande disputation 2013-11-22 Nedanstående person kommer att disputera för avläggande av teknologie doktorsexamen. Namn: Nathalie Pérez Rodríguez Ämne: Tillämpad geologi/Applied Geology Avhandling: Biotic and Abiotic Isotope Fractionation of Copper and Iron. From the Lab to the Field Scale Opponent: Dr Dominik Weiss, Reader, Department of Earth Science & Engineering, Imperial College of London, UK Ordförande: Professor Björn Öhlander, Institutionen för samhällsbyggnad och naturresurser, Luleå tekniska universitet Tid: Tisdag den 17 december 2013, kl 10.00 Plats: E632, Luleå tekniska universitetAvailable from: 2016-09-30 Created: 2016-09-30 Last updated: 2018-06-11Bibliographically approved
Rodríguez, N. P., Engström, E., Rodushkin, I., Nason, P., Alakangas, L. & Öhlander, B. (2013). Copper and iron isotope fractionation in mine tailings at the Laver and Kristineberg mines, northern Sweden (ed.). Paper presented at International Symposium on Applied Isotope Geochemistry : 19/09/2011 - 23/09/2011. Applied Geochemistry, 32, 204-215
Open this publication in new window or tab >>Copper and iron isotope fractionation in mine tailings at the Laver and Kristineberg mines, northern Sweden
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2013 (English)In: Applied Geochemistry, ISSN 0883-2927, E-ISSN 1872-9134, Vol. 32, p. 204-215Article in journal (Refereed) Published
Abstract [en]

Previous research has shown that Cu and Fe isotopes are fractionated by dissolution and precipitation reactions driven by changing redox conditions. In this study, Cu isotope composition (65Cu/63Cu ratios) was studied in profiles through sulfide-bearing tailings at the former Cu mine at Laver and in a pilot-scale test cell at the Kristineberg mine, both in northern Sweden. The profile at Kristineberg was also analysed for Fe isotope composition (56Fe/54Fe ratios). At both sites sulfide oxidation resulted in an enrichment of the lighter Cu isotope in the oxidised zone of the tailings compared to the original isotope ratio, probably due to preferential losses of the heavier Cu isotope into the liquid phase during oxidation of sulfides. In a zone with secondary enrichment of Cu, located just below the oxidation front at Laver, δ65Cu (compared to ERM-AE633) was as low as -4.35 ± 0.02‰, which can be compared to the original value of 1.31 ± 0.03‰ in the unoxidised tailings. Precipitation of covellite in the secondary Cu enrichment zone explains this fractionation. The Fe isotopic composition in the Kristineberg profile is similar in the oxidised zone and in the unoxidised zone, with average δ56Fe values (relative to the IRMM-014) of -0.58± 0.06‰ and -0.49 ± 0.05‰, respectively. At the well-defined oxidation front, δ56Fe was less negative, -0.24 ± 0.01 ‰. Processes such as Fe(II)-Fe(III) equilibrium and precipitation of Fe-(oxy)hydroxides at the oxidation front are assumed to cause this Fe isotope fractionation. This field study provides additional support for the importance of redox processes for the isotopic composition of Cu and Fe in natural systems.

National Category
Geochemistry
Research subject
Applied Geology
Identifiers
urn:nbn:se:ltu:diva-39911 (URN)10.1016/j.apgeochem.2012.10.012 (DOI)000317408400020 ()2-s2.0-84876319000 (Scopus ID)ed4fd2fd-964e-47a4-84de-ed2fce71ed78 (Local ID)ed4fd2fd-964e-47a4-84de-ed2fce71ed78 (Archive number)ed4fd2fd-964e-47a4-84de-ed2fce71ed78 (OAI)
Conference
International Symposium on Applied Isotope Geochemistry : 19/09/2011 - 23/09/2011
Note
Validerad; 2013; 20121029 (andbra); Konferensartikel i tidskriftAvailable from: 2016-10-03 Created: 2016-10-03 Last updated: 2019-11-22Bibliographically approved
Rodríguez, N. P., Neagoe, A. & Öhlander, B. (2013). Experimental scales as a factor for copper isotope fractionation (ed.). Paper presented at Applied Isotope Geochemistry Conference : 22/09/2013 - 27/09/2013. Central European Geology, 56(2-3), 118-121
Open this publication in new window or tab >>Experimental scales as a factor for copper isotope fractionation
2013 (English)In: Central European Geology, ISSN 1788-2281, Vol. 56, no 2-3, p. 118-121Article in journal, Meeting abstract (Refereed) Published
National Category
Geochemistry
Research subject
Applied Geology
Identifiers
urn:nbn:se:ltu:diva-27194 (URN)10.1556/CEuGeol.56.2013.2-3.1 (DOI)08a3fc6f-5b7b-4401-8bc3-1b31a4526cad (Local ID)08a3fc6f-5b7b-4401-8bc3-1b31a4526cad (Archive number)08a3fc6f-5b7b-4401-8bc3-1b31a4526cad (OAI)
Conference
Applied Isotope Geochemistry Conference : 22/09/2013 - 27/09/2013
Note

Godkänd; 2013; 20130930 (natper)

Available from: 2016-09-30 Created: 2016-09-30 Last updated: 2018-11-27Bibliographically approved
Rodríguez, N. P., Adlassnig, W., Rodushkin, I., Alakangas, L. & Öhlander, B. (2013). Isotopic fractionation of Cu in biofilms from a historic mining site (ed.). Paper presented at Annual V.M. Goldschmidt Conference : 25/08/2013 - 30/08/2013. Mineralogical magazine, 77(5), 1950
Open this publication in new window or tab >>Isotopic fractionation of Cu in biofilms from a historic mining site
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2013 (English)In: Mineralogical magazine, ISSN 0026-461X, E-ISSN 1471-8022, Vol. 77, no 5, p. 1950-Article in journal, Meeting abstract (Refereed) Published
National Category
Geochemistry
Research subject
Applied Geology
Identifiers
urn:nbn:se:ltu:diva-38220 (URN)10.1180/minmag.2013.077.5.16 (DOI)c894fccf-7fdc-48b6-8cb5-a74e9952ebec (Local ID)c894fccf-7fdc-48b6-8cb5-a74e9952ebec (Archive number)c894fccf-7fdc-48b6-8cb5-a74e9952ebec (OAI)
Conference
Annual V.M. Goldschmidt Conference : 25/08/2013 - 30/08/2013
Projects
Using MicroBes for the REgulation of heavy metaL mobiLity at ecosystem and landscape scAle: an integrative approach for soil remediation by geobiological processes
Note
Godkänd; 2013; 20130814 (ysko)Available from: 2016-10-03 Created: 2016-10-03 Last updated: 2019-11-22Bibliographically approved
Rodríguez, N. P. (2012). Fractionation of Cu and Fe isotopes in metal-rich mine sites: biotic and abiotic processes (ed.). (Licentiate dissertation). Paper presented at . Luleå: Luleå tekniska universitet
Open this publication in new window or tab >>Fractionation of Cu and Fe isotopes in metal-rich mine sites: biotic and abiotic processes
2012 (English)Licentiate thesis, comprehensive summary (Other academic)
Abstract [en]

After mineral exploitation the residual grinded and milled material, rich in sulphide minerals and heavy metals, is often left exposed to the atmospheric variables. This weathered mine waste material can lead to the formation of acid mine drainage (AMD) which has negative effects to the environment. The fractionation of stable isotope of metals such as Cu and Fe can be measured using innovative analytical techniques developed recently and could offer a detailed hindsight of the geochemical processes occurring in mine contaminated sites. Tailings profiles from Northern Sweden with high content of Cu and Fe sulphides and in different stages of weathering and/or remediation, along with plant and soil samples from a phytoremediation test site in Ronneburg, Germany were analysed using MC-ICP-MS to measure the isotope ratios of 65Cu/63Cu and 56Fe/54Fe. The analytical method used requires anion exchange chromatography to extract Cu and Fe from a complex matrix prior to the proper isotope ratio measurement. The samples from the tailings profile were useful to interpret the geochemical processes that can lead to a fractionation of Cu and Fe in the field, since redox-driven reactions such as rock oxidation and mineral precipitation are present in such environment. This study shows that precipitation of covellite in a redox-boundary zone in a mine tailings can cause a clear fractionation of Cu (Δ65Curock-covellite= -5.66±0.05‰) and a depletion of the lighter Cu isotope in the oxidised areas of the tailings due to dissolution of the remaining Cu-sulphides. Precipitation of Fe(oxy)hydroxides as a result of the oxidation process of sulphide-bearing rocks can also fractionate Fe, being the precipitated mineral slightly enriched in 56Fe.The influence of soil bacteria and plant uptake in the fractionation of Cu and Fe was investigated in pot and field experiments at the Ronneburg site, where organic amendments were used. The results showed that the plant material was enriched in the lighter Fe isotope compared to the substrate used in the pot and field experiments, in spite of the application of a bacterial consortium. Cu isotope fractionation is more susceptible to the changes in the amendments used, being those bacterial consortium, mychorriza or compost than Fe isotope fractionation. There are differences in the fractionation values in pot and field trials, regardless of the type of organic amendment applied. As an overall view, leaves are enriched in the heavier Cu isotope compared to the soils, regardless of the amendment usedThe application of the results obtained in this work would help not only to offer a view in the cycle of Fe and Cu in the surface environment, and the understanding of the (bio)geochemical processes occurring in sulphide soil surfaces. But also in the way that current remediation techniques of metal contaminated sites could be evaluated, having in mind that simplified systems show a different Cu and Fe fractionation compared to natural systems where more variables are needed to take into account.

Place, publisher, year, edition, pages
Luleå: Luleå tekniska universitet, 2012
Keywords
Cu and Fe isotopes, fractionation, tailings, plants, phytoremediation, organic amendments, soil bacteria, Cu and Fe cycle, covellite, redox processes, Earth sciences - Exogenous eart sciences, Geovetenskap - Exogen geovetenskap
National Category
Geochemistry
Research subject
Applied Geology
Identifiers
urn:nbn:se:ltu:diva-26219 (URN)d325f181-13e7-4848-9ac1-9b0b66545376 (Local ID)978-91-7439-470-2 (ISBN)d325f181-13e7-4848-9ac1-9b0b66545376 (Archive number)d325f181-13e7-4848-9ac1-9b0b66545376 (OAI)
Note
Godkänd; 2012; 20120816 (natper); LICENTIATSEMINARIUM Ämnesområde: Tillämpad geologi/Applied Geology Uppsats: Fractionation of Cu and Fe Isotopes in Metal-Rich Mine sites: Biotic and Abiotic Processes Examinator: Professor Björn Öhlander, Institutionen för samhällsbyggnad och naturresurser, Luleå tekniska universitet Diskutant: Dr., Project Manager Magnus Land, Kungliga Vetenskapsakademien Tid: Tisdag den 25 september 2012 kl 10.00 Plats: F341, Luleå tekniska universitetAvailable from: 2016-09-30 Created: 2016-09-30 Last updated: 2018-06-11Bibliographically approved
Öhlander, B., Alakangas, L. & Perez, N. (2012). Project: Using MicroBes for the Regulation of heavy metaL mobiLity at ecosystem and landscape scAle: an integrative approach for soil remediation by geobiological processes. Paper presented at .
Open this publication in new window or tab >>Project: Using MicroBes for the Regulation of heavy metaL mobiLity at ecosystem and landscape scAle: an integrative approach for soil remediation by geobiological processes
2012 (English)Other (Other (popular science, discussion, etc.))
National Category
Geochemistry
Research subject
Applied Geology
Identifiers
urn:nbn:se:ltu:diva-36212 (URN)b061a15b-215e-4e27-8204-5b1764ee4f50 (Local ID)b061a15b-215e-4e27-8204-5b1764ee4f50 (Archive number)b061a15b-215e-4e27-8204-5b1764ee4f50 (OAI)
Note

Publikationer: Isotopic fractionation of Cu in biofilms from a historic mining site; The role of bacterial consortium and organic amendment in Cu and Fe isotope fractionation in plants on a polluted mine site ; Status: Pågående; Period: 01/05/2009 → 31/05/2012

Available from: 2016-09-30 Created: 2016-09-30 Last updated: 2019-11-22Bibliographically approved
Rodríguez, N. P., Nason, P., Alakangas, L. & Öhlander, B. (2011). Copper and iron isotopes in mine tailings and their relationship to weathering processes (ed.). Paper presented at International Applied Geochemistry Symposium : 22/08/2011 - 26/08/2011. Paper presented at International Applied Geochemistry Symposium : 22/08/2011 - 26/08/2011.
Open this publication in new window or tab >>Copper and iron isotopes in mine tailings and their relationship to weathering processes
2011 (English)Conference paper, Oral presentation only (Other academic)
National Category
Geochemistry
Research subject
Applied Geology
Identifiers
urn:nbn:se:ltu:diva-28554 (URN)2621316a-7e7e-4d5f-ab17-bbe8798e80f1 (Local ID)2621316a-7e7e-4d5f-ab17-bbe8798e80f1 (Archive number)2621316a-7e7e-4d5f-ab17-bbe8798e80f1 (OAI)
Conference
International Applied Geochemistry Symposium : 22/08/2011 - 26/08/2011
Note
Godkänd; 2011; 20111013 (natper)Available from: 2016-09-30 Created: 2016-09-30 Last updated: 2019-11-22Bibliographically approved
Rodríguez, N. P., Nason, P., Alakangas, L. & Öhlander, B. (2011). Copper isotope fractionation in mine tailings in northern Sweden. (ed.). Paper presented at International Symposium on Applied Isotope Geochemistry : 19/09/2011 - 23/09/2011. Paper presented at International Symposium on Applied Isotope Geochemistry : 19/09/2011 - 23/09/2011.
Open this publication in new window or tab >>Copper isotope fractionation in mine tailings in northern Sweden.
2011 (English)Conference paper, Oral presentation only (Refereed)
National Category
Geochemistry
Research subject
Applied Geology
Identifiers
urn:nbn:se:ltu:diva-39456 (URN)e3aad202-5209-43a5-969b-d62a0b2657d1 (Local ID)e3aad202-5209-43a5-969b-d62a0b2657d1 (Archive number)e3aad202-5209-43a5-969b-d62a0b2657d1 (OAI)
Conference
International Symposium on Applied Isotope Geochemistry : 19/09/2011 - 23/09/2011
Note
Godkänd; 2011; 20111013 (natper)Available from: 2016-10-03 Created: 2016-10-03 Last updated: 2019-11-22Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-9389-0289

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