In this paper, digital holographic (DH) microscopy demonstrates its ability to perform a full characterization of nanofibers. The high resolution and magnification of the presented method to study the nanofibers are tested using standard MIL-STD-150A 1951 USAF resolution test target. In this investigation, aggregated natural cellulose nanowhisker fibers are positioned in the front of the microscopic objective using a 3D translation stage in the object arm of DH setup. The recorded off-axis holograms are refocused using the angular spectrum method. The reconstructed complex field is used to calculate optical phase and intensity distributions of the object at different reconstruction depths. A simple algorithm is used to define the focused image with suitable accuracy. The dimensions and orientation of the fibers can be evaluated from the optical field at different depths. Then, the shape and textures along the aggregated natural cellulose nanowhisker fiber can be presented in a 3D space.

The advantages of digital holographic microscopy to record not only the intensity but also the optical phase are employed. The experimental arrangement comprises a Mach-Zehnder type interferometer with a microscopic objective of magnification 100x. The used camera is a 5 Mpixels Allied Vision Guppy Pro F-503 with a pixel pitch of 2.2 μm. The lateral magnification is set to about 200x based on the standard MIL-STD-150A 1951 USAF resolution test target. The dimensions of the aggregated natural cellulose nanowhisker fibers used are in the range of some hundreds of nanometers, which are positioned in the front of the microscopic objective using a 3D translation stage in the object arm of the holographic setup. The recorded off-axis holograms are refocused using the angular spectrum method. The reconstructed complex field is used to calculate optical phase and intensity distributions of the object at different reconstructions depths. The dimensions and orientation of the fibers can be evaluated from the optical field at different depths. Then, the shape and textures along the aggregated natural cellulose nanowhisker fiber can be presented in 3D space. The nano fiber found to have the dimensions of mean width 223 nm, depth 308 nm and length of 8.1 μm. Further, the mean local refractive index of the nano fibers can be calculated (n=1.501).

Light absorbing objects embedded in silicone have been imaged using photoacoustic digital holography. The photoacoustic waves were generated using a pulsed Nd:YAG laser, λ=1064 nm, and pulse length=12 ns. When the waves reached the silicone surface, they were measured optically along a line using a scanning laser vibrometer. The acoustic waves were then digitally reconstructed using a holographic algorithm. The laser vibrometer is proven to be sensitive enough to measure the surface velocity due to photoacoustic waves generated from laser pulses with a fluence allowed for human tissue. It is also shown that combining digital holographic reconstructions for different acoustic wavelengths provides images with suppressed noise and improved depth resolution. The objects are imaged at a depth of 16.5 mm with a depth resolution of 0.5 mm.

technique to gain depth information from a single pair image-plane Digital Holographic recording of a transient phase object positioned between a diffuser and an imaging system has been demonstrated

This study is focused on exploring the feasibility of an all-optic surface scanning method in determining the size and position of a submerged, laser generated, optoacoustic source. The optoacoustic effect was here generated when the absorption of a short electromagnetic pulse in matter caused a dielectric breakdown, a plasma emission flash and a subsequent acoustic wave. In the experiment, a laser pulse with l = 1064 nm and 12 ns pulse length was aimed at a volume of deionized water. When the laser beam was focused by a f = 16 mm lens, a single dielectric breakdown spot occurred. When a f = 40 mm was used several breakdowns in a row were induced. The breakdowns were photographed using a double shutter camera. The acoustic wave generated by the dielectric breakdowns were detected at a point on the water surface using a laser Doppler vibrometer (LDV). First, the LDV signal was used to calculate the speed of sound with an accuracy of 10 m/s. Secondly, the location and length of the dielectric breakdown was calculated with an accuracy of 1 mm. The calculated position matched the breakdown location recorded by a camera. The results show that it is possible to use LDV surface measurements from a single spot to determine both the position and length of the OA source as well as the speed of sound in the medium. Furthermore, the LDV measurements also show a secondary peak that originates from the OA source. To unravel the origin and properties of this interesting feature, further investigations are necessary.

We studied photoacoustic waves using pulsed digital holography. The acoustic waves were generated in a reindeer blood target by absorption of an IR laser pulse, λ=1064 nm and pulse length=12 ns. The acoustic pressure waves were then imaged in water using a second collimated laser pulse at λ=532 nm2 μs after the first IR pulse. Quantitative information on acoustic wave properties such as three-dimensional shape and pressure distribution was calculated by applying the inverse Radon transform on the recorded projection. The pressure pulse had a flat and sharp front parallel with the blood surface, which indicates that the pressure was generated at the blood surface. The generated pressure was proportional to the laser fluence with the proportionality constant equal to 1.8±0.3 cm-1. According to existing data, the proportionality constant should be 1.4 cm-1 for oxygenated human blood, which made our calculations probable

Digital holographic and tomographic reconstruction algorithms have been used for imaging of sound sources. Making the tomographic reconstruction first produces images with higher quality. Higher resolution and selective imaging is obtained by using multiple frequencies.

A technique to gain depth information from an image-plane digital holographic recording of a transient phase object positioned between a diffuser and an imaging system is demonstrated. The technique produces telecentric reconstructions of the complex amplitude throughout the phase volume using numerical lenses and the complex spectrum formulation of the diffraction integral. The in-plane speckle movements as well as the phase difference between the disturbed field and a reference field are calculated in a finite number of planes using a cross-correlation formulation. It is shown that depth information about in-plane phase gradients can be determined in two planes using reconstructed speckle fields from four different depths. In addition, the plane of optimum reconstruction for calculating the phase difference with maximum contrast is detected from the technique. The method is demonstrated on a measurement of a laser ablation process.