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Ahmed, N., Siow, K. S., Wee, M. F. & Patra, A. (2023). A study to examine the ageing behaviour of cold plasma-treated agricultural seeds. Scientific Reports, 13, Article ID 1675.
Open this publication in new window or tab >>A study to examine the ageing behaviour of cold plasma-treated agricultural seeds
2023 (English)In: Scientific Reports, E-ISSN 2045-2322, Vol. 13, article id 1675Article in journal (Refereed) Published
Abstract [en]

Cold plasma (low pressure) technology has been effectively used to boost the germination and growth of various crops in recent decades. The durability of these plasma-treated seeds is essential because of the need to store and distribute the seeds at different locations. However, these ageing effects are often not ascertained and reported because germination and related tests are carried out within a short time after the plasma-treatment. This research aims to fill that knowledge gap by subjecting three different types of seeds (and precursors): Bambara groundnuts (water), chilli (oxygen), and papaya (oxygen) to cold plasma-treatment. Common mechanisms found for these diverse seed types and treatment conditions were the physical and chemical changes induced by the physical etching and the cold plasma on the seeds and subsequent oxidation, which promoted germination and growth. The high glass transition temperature of the lignin-cellulose prevented any physical restructuring of the surfaces while maintaining the chemical changes to continue to promote the seeds germination and growth. These changes were monitored over 60 days of ageing using water contact angle (WCA), water uptake, electrical conductivity, field emission scanning electron microscopy (FE-SEM) and X-ray photoelectron spectroscopy (XPS). The vacuum effect was also investigated to separate its effect from cold plasma (low pressure). This finding offers a framework for determining how long agricultural seeds that have received plasma treatment can be used. Additionally, there is a need to transfer this research from the lab to the field. Once the impact of plasma treatment on seeds has been estimated, it will be simple to do so.

Place, publisher, year, edition, pages
Springer Nature, 2023
Keywords
Chemistry, Engineering, Plant sciences
National Category
Organic Chemistry Biochemistry and Molecular Biology
Research subject
Chemistry of Interfaces
Identifiers
urn:nbn:se:ltu:diva-95458 (URN)10.1038/s41598-023-28811-w (DOI)36717647 (PubMedID)2-s2.0-85147028152 (Scopus ID)
Funder
The Kempe Foundations
Note

Validerad;2023;Nivå 2;2023-03-13 (hanlid);

Funder: Malaysia Ministry of Education (FRGS-1-2019-STG07-UKM/02/9); PRECISE LTU

Available from: 2023-01-31 Created: 2023-01-31 Last updated: 2023-05-08Bibliographically approved
Masood, A., Ahmed, N., Razip Wee, M. F., Patra, A., Mahmoudi, E. & Siow, K. S. (2023). Atmospheric Pressure Plasma Polymerisation of D-Limonene and Its Antimicrobial Activity. Polymers, 15(2), Article ID 307.
Open this publication in new window or tab >>Atmospheric Pressure Plasma Polymerisation of D-Limonene and Its Antimicrobial Activity
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2023 (English)In: Polymers, E-ISSN 2073-4360, Vol. 15, no 2, article id 307Article in journal (Refereed) Published
Abstract [en]

Antibacterial coating is necessary to prevent biofilm-forming bacteria from colonising medical tools causing infection and sepsis in patients. The recent coating strategies such as immobilisation of antimicrobial materials and low-pressure plasma polymerisation may require multiple processing steps involving a high-vacuum system and time-consuming process. Some of those have limited efficacy and durability. Here, we report a rapid and one-step atmospheric pressure plasma polymerisation (APPP) of D-limonene to produce nano-thin films with hydrophobic-like properties for antibacterial applications. The influence of plasma polymerisation time on the thickness, surface characteristic, and chemical composition of the plasma-polymerised films was systematically investigated. Results showed that the nano-thin films deposited at 1 min on glass substrate are optically transparent and homogenous, with a thickness of 44.3 ± 4.8 nm, a smooth surface with an average roughness of 0.23 ± 0.02 nm. For its antimicrobial activity, the biofilm assay evaluation revealed a significant 94% decrease in the number of Escherichia coli (E. coli) compared to the control sample. More importantly, the resultant nano-thin films exhibited a potent bactericidal effect that can distort and rupture the membrane of the treated bacteria. These findings provide important insights into the development of bacteria-resistant and biocompatible coatings on the arbitrary substrate in a straightforward and cost-effective route at atmospheric pressure.

Place, publisher, year, edition, pages
MDPI, 2023
Keywords
atmospheric pressure, plasma polymerisation, D-limonene, ASTM E2149, antimicrobial coating, E. coli bacteria
National Category
Other Chemistry Topics
Research subject
Chemistry of Interfaces
Identifiers
urn:nbn:se:ltu:diva-95375 (URN)10.3390/polym15020307 (DOI)000918902200001 ()36679188 (PubMedID)2-s2.0-85146665863 (Scopus ID)
Note

Validerad;2023;Nivå 2;2023-01-24 (johcin);

Funding: Universiti Kebangsaan Malaysia (UKM); Malaysia under Geran Universiti Penyelidikan (GUP-2022-071)

Available from: 2023-01-24 Created: 2023-01-24 Last updated: 2024-01-17Bibliographically approved
Masood, A., Ahmed, N., Shahid, F., Wee, M. F., Patra, A. & Siow, K. S. (2023). Atmospheric Pressure Plasma Polymerization of Carvone: A Promising Approach for Antimicrobial Coatings. Coatings, 13(6), Article ID 1112.
Open this publication in new window or tab >>Atmospheric Pressure Plasma Polymerization of Carvone: A Promising Approach for Antimicrobial Coatings
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2023 (English)In: Coatings, ISSN 2079-6412, Vol. 13, no 6, article id 1112Article in journal (Refereed) Published
Abstract [en]

Medical devices are often vulnerable to colonization by nosocomial pathogens (bacteria), leading to infections. Traditional sterilization methods may not always be effective, and as a result, alternative options are being explored to prevent microbial contamination. Recently, scientists are emphasizing using plant-derived essential oils that possess inherent antibacterial properties to produce antimicrobial coatings using plasma polymerization technology carried out at atmospheric pressure (AP). This approach shows promise compared to other coating strategies that need several processing steps, including a high-vacuum system, and are laborious, such as the immobilization of antimicrobial materials on precoated layers in the low-pressure plasma polymerization approach. The present study demonstrates the potential of AP plasma polymerization for producing thin films with excellent antibacterial properties and surface characteristics. The resulting coatings are stable, smooth, and have high wettability, making them ideal for repelling bacteria. The calculated zeta potential and deposition rate for the films are also favorable. These AP plasma-polymerized thin films created from carvone show a reduction rate of more than 90% for Escherichia coli and Staphylococcus aureus bacteria. Our computational docking studies also reveal strong binding interactions between the original carvone monomer and both bacteria. The study suggests that these AP plasma-produced coatings have great potential as antibacterial coatings for biomedical devices.

Place, publisher, year, edition, pages
Mdpi, 2023
Keywords
atmospheric pressure plasma polymerization, antibacterial coating, carvone, ASTM E2149-20, E, coli, S, aureus
National Category
Medicinal Chemistry
Research subject
Chemistry of Interfaces
Identifiers
urn:nbn:se:ltu:diva-99293 (URN)10.3390/coatings13061112 (DOI)001017079700001 ()2-s2.0-85163801747 (Scopus ID)
Note

Validerad;2023;Nivå 2;2023-08-08 (joosat);

Funder: Universiti Kebangsaan Malaysia (GUP-2022-071); PRECISE LTU

Licens fulltext: CC BY License

Available from: 2023-08-08 Created: 2023-08-08 Last updated: 2023-08-08Bibliographically approved
Dey, S., Guchhait, K. C., Jana, D., Majumder, S., Patra, A., Panda, A. K. & Ghosh, C. (2023). Biosynthesis of Lantibiotics (1ed.). In: Sanket Joshi, Rajiv Kar, Dibyajit Lahiri, Moupriya Nag (Ed.), Lantibiotics as Alternative Therapeutics: (pp. 43-63). Academic Press
Open this publication in new window or tab >>Biosynthesis of Lantibiotics
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2023 (English)In: Lantibiotics as Alternative Therapeutics / [ed] Sanket Joshi, Rajiv Kar, Dibyajit Lahiri, Moupriya Nag, Academic Press, 2023, 1, p. 43-63Chapter in book (Refereed)
Abstract [en]

In the last few decades, the prevalence of multidrug resistance and importunate virulence in bacteria emerged as one of the serious concerns in health-care system. Lantibiotics, the well-conserved antimicrobial peptides predominantly rich in atypical sulfur-containing amino acids, lanthionine (Lan), and 3-methyl-lanthionine (MeLan), synthesized ribosomally from bacterial cells and modified posttranslationally, have drawn considerable interest in the therapeutic management of infectious diseases because of their effectiveness against multidrug-resistant bacteria. So far studied, the other reasons remain their potency, broad-spectrum nature, and negligible cytotoxicity. The biosynthesis of the lantibiotics involves two major steps, the modification of precursor peptides and their proteolytic activation. The gene cluster constituting the biosynthetic machinery codes essentially for the prepeptide and modification enzymes besides the proteases, ATP-binding cassette transporters, immunity factors, and regulatory proteins required as accessory factors. Herein, in this chapter a comprehensive discussion has been made on the biosynthesis of lantibiotics, which may be noteworthy in biomedical science for technology advancement.

Place, publisher, year, edition, pages
Academic Press, 2023 Edition: 1
Series
Advances in Biotechnology and Bioengineering
Keywords
Lantibiotics, biosynthesis, lanthionine, prepeptide, gene cluster, multidrug resistance
National Category
Other Chemistry Topics
Research subject
Chemistry of Interfaces
Identifiers
urn:nbn:se:ltu:diva-95371 (URN)10.1016/B978-0-323-99141-4.00014-X (DOI)2-s2.0-85152848183 (Scopus ID)9780323991421 (ISBN)9780323991414 (ISBN)
Available from: 2023-01-24 Created: 2023-01-24 Last updated: 2023-10-11Bibliographically approved
Roy, B., Guha, P., Chang, C.-H., Nahak, P., Karmakar, G., Bykov, A. G., . . . Panda, A. K. (2023). Effect of cationic dendrimer on membrane mimetic systems in the form of monolayer and bilayer. Chemistry and Physics of Lipids, Article ID 105364.
Open this publication in new window or tab >>Effect of cationic dendrimer on membrane mimetic systems in the form of monolayer and bilayer
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2023 (English)In: Chemistry and Physics of Lipids, ISSN 0009-3084, E-ISSN 1873-2941, article id 105364Article, review/survey (Refereed) Epub ahead of print
Place, publisher, year, edition, pages
Elsevier, 2023
National Category
Physical Chemistry
Research subject
Chemistry of Interfaces
Identifiers
urn:nbn:se:ltu:diva-103153 (URN)10.1016/j.chemphyslip.2023.105364 (DOI)
Funder
Luleå University of TechnologyThe Kempe Foundations
Note

Funder: Council of Scientific & Industrial Research (project no.01(2533)/11/EMR-II); University Grant Commission (Basic Scientific Research-UGC), Government of India, New Delhi;

Available from: 2023-12-04 Created: 2023-12-04 Last updated: 2023-12-04
Ghosh, A., Kumar De, S., Mondal, S., Halder, A., Barai, M., Chandra Guchhait, K., . . . Kumar Sur, U. (2023). Green synthesis of silver nanoparticles and its applications as sensor, catalyst, and antibacterial agent. Materials Today: Proceedings
Open this publication in new window or tab >>Green synthesis of silver nanoparticles and its applications as sensor, catalyst, and antibacterial agent
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2023 (English)In: Materials Today: Proceedings, E-ISSN 2214-7853Article in journal (Refereed) Epub ahead of print
Abstract [en]

We have reported for the first time a solvated electron mediated reduction of silver ion (Ag+) in presence of extract of Murraya koenigii (Mk) leaf as a surfactant to produce silver nanoparticles (AgNPs). Synthesized NPs show efficient ascorbic acid sensing at the µM range along with selective detection of different metal ions due to the presence of different biomolecules on the surface which are present in the extract. Synthesized nontoxic AgNPs will also exhibit proficient photocatalytic behavior for the degradation of toxic dyes due to the effective coupling between surface plasmon of AgNPs and the excited state continuum of the dye fluorophores. AgNPs will also exhibit substantial antibacterial activities. Altogether, our biosynthesized nontoxic NPs could be effectively used as both chemical and biosensor, catalyst as well as antibacterial agent.

Place, publisher, year, edition, pages
Elsevier, 2023
Keywords
Coherent capacitive coupling, Green synthesis, Metal sensing, Photo catalysis/degradation, Solvated electron
National Category
Materials Chemistry Analytical Chemistry
Research subject
Chemistry of Interfaces
Identifiers
urn:nbn:se:ltu:diva-96271 (URN)10.1016/j.matpr.2023.03.159 (DOI)2-s2.0-85150284309 (Scopus ID)
Note

Funder: Department of Higher Education, Science, Technology and Biotechnology), Government of West Bengal (161, T/9G-50/2017); Department of Biotechnology; DST-FIST;

Available from: 2023-04-05 Created: 2023-04-05 Last updated: 2023-04-05
Ahmed, N., Masood, A., Mumtaz, R., Wee, M. F., Chan, K. M., Patra, A. & Siow, K. S. (2023). Quad-atmospheric Pressure Plasma Jet (q-APPJ) Treatment of Chilli Seeds to Stimulate Germination. Plasma chemistry and plasma processing
Open this publication in new window or tab >>Quad-atmospheric Pressure Plasma Jet (q-APPJ) Treatment of Chilli Seeds to Stimulate Germination
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2023 (English)In: Plasma chemistry and plasma processing, ISSN 0272-4324, E-ISSN 1572-8986Article in journal (Refereed) Epub ahead of print
Place, publisher, year, edition, pages
Springer, 2023
Identifiers
urn:nbn:se:ltu:diva-103502 (URN)10.1007/s11090-023-10436-6 (DOI)2-s2.0-85178877054 (Scopus ID)
Note

Funder: Malaysia Ministry of Education (FRGS-1-2019-STG07-UKM/02/9)

Available from: 2024-01-08 Created: 2024-01-08 Last updated: 2024-01-08
Guchhait, K. C., Manna, T., Barai, M., Karmakar, M., Nandi, S. K., Jana, D., . . . Ghosh, C. (2022). Antibiofilm and anticancer activities of unripe and ripe Azadirachta indica (neem) seed extracts. BMC Complementary Medicine and Therapies, 22(1), Article ID 42.
Open this publication in new window or tab >>Antibiofilm and anticancer activities of unripe and ripe Azadirachta indica (neem) seed extracts
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2022 (English)In: BMC Complementary Medicine and Therapies, E-ISSN 2662-7671, Vol. 22, no 1, article id 42Article in journal (Refereed) Published
Abstract [en]

Background

Antibiotic resistances of pathogens and breast cancer warrant the search for new alternative strategies. Phytoextracts can eradicate microbe-borne diseases as well as cancer with lower side effects compared to conventional antibiotics.

Aim

Unripe and ripe Azadirachta indica (neem) seed extracts were explored as potential antibiofilm and anticancer agents in combating multidrug-resistant infectious bacteria as well as anticancer agents against the MDR breast cancer cell lines.

Methods

Shed-dried neem seeds (both unripe and ripe) were pulverized and extracted using methanol. The chemical components were identified with FTIR and gas chromatography - mass spectrometry. Antibiofilm activity of neem seed extracts were assessed in terms of minimum biofilm inhibitory concentration (MBIC), minimum biofilm eradication concentration (MBEC), and fluorescence microscopic studies on Staphylococcus aureus and Vibrio cholerae. Bacterial cells were studied by fluorescence microscopy using acridine orange/ethidium bromide as the staining agents. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were evaluated to observe the antibacterial activities. Cytotoxicity of the extracts against human blood lymphocytes and the anticancer activity against drug-resistant breast cancer cell lines were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and fluorescence-activated cell sorting (FACS) studies.

Results

4-Ethyl-2-hydroxy-2-cyclopentene-1-one, phthalic acid, and 2-hexyl-tetrahydro thiophane were the major compounds in unripe neem seed, whereas 3,5-dihydroxy-6-methyl-2,3-dihydro-4-H-pyran-4-one and 4-ethylbenzamide were predominant in ripe neem seed. Triazine derivatives were also common for both the extracts. MBIC values of unripe and ripe neem seed extracts for S. aureus are 75 and 100 µg/mL, respectively, and for V. cholerae, they are 100 and 300 µg/mL, respectively. MBEC values of unripe and ripe seed extracts are 500 and 300 µg/mL, respectively for S. aureus and for V. cholerae the values are 700 and 500 µg/mL, respectively. Fluorescence microscopic studies at 16 and 24 h, after bacterial culture, demonstrate enhanced antibiofilm activity for the ripe seed extract than that of the unripe seeds for both the bacteria. MTT assay reveals lower cytotoxicity of both the extracts towards normal blood lymphocytes, and anticancer activity against breast cancer cell line (MDA-MB-231) with superior activity of ripe seed extract. FACS studies further supported higher anticancer activity for ripe seed extract.

Conclusions

Methanolic extract of neem seeds could substantially inhibit and eradicate biofilm along with their potent antibacterial and anticancer activities. Both the extracts showed higher antibiofilm and antibacterial activity against S. aureus (gram-positive) than V. cholerae (gram-negative). Moreover, ripe seed extract showed higher antibiofilm and anticancer activity than unripe extracts.

Place, publisher, year, edition, pages
Springer Nature, 2022
Keywords
Azadirachta indica, Bacteria, Biofilm, Cancer, Neem, S. aureus, V. cholerae
National Category
Organic Chemistry Biochemistry and Molecular Biology Medicinal Chemistry
Research subject
Chemistry of Interfaces
Identifiers
urn:nbn:se:ltu:diva-89377 (URN)10.1186/s12906-022-03513-4 (DOI)000754666800001 ()35152903 (PubMedID)2-s2.0-85124636349 (Scopus ID)
Note

Validerad;2022;Nivå 2;2022-02-24 (joosat);

Funder: University Grant Commission (UGC), India; Department of Biotechnology, Govt. of India (BT/PR3802/BRB/10/981/2011)

Available from: 2022-02-24 Created: 2022-02-24 Last updated: 2022-07-06Bibliographically approved
Ahmed, N., Masood, A., Siow, K. S., Wee, M. F., Haron, F. F., Patra, A., . . . Soon, C. F. (2022). Effects of Oxygen (O2) Plasma Treatment in Promoting the Germination and Growth of Chili. Plasma chemistry and plasma processing, 42(1), 91-108
Open this publication in new window or tab >>Effects of Oxygen (O2) Plasma Treatment in Promoting the Germination and Growth of Chili
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2022 (English)In: Plasma chemistry and plasma processing, ISSN 0272-4324, E-ISSN 1572-8986, Vol. 42, no 1, p. 91-108Article in journal (Refereed) Published
Abstract [en]

In general, seed germination is improved by low-pressure plasma treatment using precursors such as air, nitrogen, O2, and argon, etc. For the first time, low-pressure O2 plasma was used to treat chili seeds in this study. When compared to untreated and vacuum-treated seeds, O2 plasma treatment using the discharge power of 80 W for 60 s significantly improves chili seed germination and growth. The effect of vacuum on the germination and growth of chili seeds was also studied and shown to be negligible. The physical and chemical changes induced by O2 plasma treatment were investigated to understand the plasma treatment to germination improvement. Combinatory etching and chemical modification aided imbibition and increased germination percentage in this O2 plasma treatment on chili seeds. The success of this method has the potential to be scaled up to solve food security issues with seeds that would otherwise struggle to germinate.

Place, publisher, year, edition, pages
Springer, 2022
Keywords
Germination, Wettability, Water absorption, Oxygen plasma treatment, Active species
National Category
Botany
Research subject
Chemistry of Interfaces
Identifiers
urn:nbn:se:ltu:diva-87703 (URN)10.1007/s11090-021-10206-2 (DOI)000709276300001 ()2-s2.0-85117295436 (Scopus ID)
Note

Validerad;2022;Nivå 2;2022-02-25 (hanlid);

Forskningsfinansiär: Malaysia Ministry of Education (FRGS-1-2019-STG07-UKM/02/9)

Available from: 2021-11-01 Created: 2021-11-01 Last updated: 2022-03-04Bibliographically approved
Dey, S., Guchhait, K. C., Manna, T., Panda, A. K., Patra, A., Mondal, S. K. & Ghosh, C. (2022). Evolutionary and compositional analysis of streptokinase including its interaction with plasminogen: An in silico approach. Gene Reports, 29, Article ID 101689.
Open this publication in new window or tab >>Evolutionary and compositional analysis of streptokinase including its interaction with plasminogen: An in silico approach
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2022 (English)In: Gene Reports, ISSN 2452-0144, Vol. 29, article id 101689Article in journal (Refereed) Published
Abstract [en]

Due to the accumulation of cholesterol in arterial wall, clot-forming cascade activated in the blood capillaries lead to conditions like myocardial ischemia and heart failure. These clots are dissolved by the enzyme streptokinase (SK), produced by Streptococcus sp., a normal flora in the human body. In the present study, codon-dependent evolution of twenty-three SK from Streptococcus sp. bacteria, isolated from distinct geographical origins were analyzed. Besides, domain variation, compositional analysis, effective number of codons (ENc) plot, codon adaptation index (CAI), and effect of polymorphism on SK interaction with its substrate plasminogen (Plg) were analyzed. Codon usage bias varied within a tiny range. Studies also reveal that AT ending codons are preferred over GC ending codons. Analyses of the other parameters reveal that the mutational pressure is one of the main factors to shape codon usage biasness. Interfacing and polar contact forming amino acid residues of SK with Plg is identical for all Streptococcus dysgalactiae but not for all Streptococcus pyogenes. Changes of those interfacing amino acid sites exhibit a substantial effect on polar interaction between SK and Plg. The present in silico studies are expected to shed further light to characterize and moreover, to understand the various contributing factors to influence SK adaptation that will be fulfilling to develop efficient thrombolytic therapeutics.

Place, publisher, year, edition, pages
Elsevier, 2022
Keywords
Streptokinase, Plasminogen, Multiple sequence alignment, Phylogenetic tree, Codon usage bias, Molecular docking
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry of Interfaces
Identifiers
urn:nbn:se:ltu:diva-93454 (URN)10.1016/j.genrep.2022.101689 (DOI)000869762500005 ()2-s2.0-85139297857 (Scopus ID)
Note

Validerad;2022;Nivå 2;2022-11-10 (hanlid);

Funder: University Grants Commission (UGC), India

Available from: 2022-10-05 Created: 2022-10-05 Last updated: 2022-11-10Bibliographically approved
Organisations
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ORCID iD: ORCID iD iconorcid.org/0000-0001-9153-6262

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