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Publications (10 of 15) Show all publications
Wei, M., Xiong, S., Chen, F., Geladi, P., Eilertsen, L., Myronycheva, O., . . . Thyrel, M. (2020). Energy smart hot-air pasteurisation as effective as energy intense autoclaving for fungal preprocessing of lignocellulose feedstock for bioethanol fuel production. Renewable energy, 155, 237-247
Open this publication in new window or tab >>Energy smart hot-air pasteurisation as effective as energy intense autoclaving for fungal preprocessing of lignocellulose feedstock for bioethanol fuel production
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2020 (English)In: Renewable energy, ISSN 0960-1481, E-ISSN 1879-0682, Vol. 155, p. 237-247Article in journal (Refereed) Published
Abstract [en]

This study compared the effects of hot-air pasteurisation (HAP) at 75–100 °C versus autoclaving at 121 °C and 2 bar overpressure on the lignocellulosic degradation process of birch-based substrates that were used for shiitake mushroom cultivation and potential bioethanol production. Fifty substrate samples were obtained as a time series from different stages of the cultivation, and their chemical contents were measured by chemical analysis and near infra-red spectroscopy (NIR). Despite of different energy intensities, HAP and autoclaving did not result in significant differences in the degradation of lignin and carbohydrates. Major compositional changes were associated with the cultivation process. Principal component analysis on the wet chemical data and orthogonal projections to latent structures based on NIR spectra reached the same conclusion, namely that HAP had similar effect as autoclaving on compositional changes in the substrate during cultivation. The results of this study suggest that a substitution of autoclaving by HAP may potentially save up to 9.9 TWh energy for the global production of 7.5 million ton shiitake. At the same time, lignocellulose feedstock can be pretreated for the production of up to 3.24 million m3 of 95%-ethanol fuels, which can potentially substitute proximate 1.88 million m3 of regular gasoline.

Place, publisher, year, edition, pages
Elsevier, 2020
Keywords
Edible mushroom, Substrate composition, Lignin, Carbohydrate, Biological pretreatment, Food and fuel
National Category
Other Mechanical Engineering
Research subject
Wood Science and Engineering
Identifiers
urn:nbn:se:ltu:diva-78347 (URN)10.1016/j.renene.2020.03.154 (DOI)2-s2.0-85082794401 (Scopus ID)
Note

Validerad;2020;Nivå 2;2020-04-23 (johcin)

Available from: 2020-04-06 Created: 2020-04-06 Last updated: 2020-04-23Bibliographically approved
Myronycheva, O., Poohphajai, F., Sehlstedt-Persson, M., Vikberg, T., Karlsson, O., Junge, H. & Sandberg, D. (2019). Application of GRAS Compounds for the Control of Mould Growth on Scots Pine Sapwood Surfaces: Multivariate Modelling of Mould Grade. Forests, 10(9), Article ID 714.
Open this publication in new window or tab >>Application of GRAS Compounds for the Control of Mould Growth on Scots Pine Sapwood Surfaces: Multivariate Modelling of Mould Grade
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2019 (English)In: Forests, ISSN 1999-4907, E-ISSN 1999-4907, Vol. 10, no 9, article id 714Article in journal (Refereed) Published
Abstract [en]

Research Highlights: In this study, the Generally Recognised as Safe (GRAS) compounds were applied in order to study mould-fungi growth on dried Scots pine sapwood. Background and Objectives: The transition to the use of more sustainable wood-material may be possible by applying GRAS compounds that can control and prevent contamination by primary colonising mould fungi. Materials and Methods: Kiln-dried sawn timber was treated with three different GRAS compounds, and different fungal inoculation methods applied in order to investigate differences in the development of fungal communities. Results: Substances based on potassium silicate significantly reduced fungal growth and mould contamination on the studied wood surfaces. By combining wood-surface treatments with GRAS compounds, fungal-area size as predictors and mould grade as response, a partial least squares (PLS) model that makes it possible to predict mould grade on wood surfaces was developed. The PLS model is a key component in the development of a smart grading-systems equipped by e.g. high-speed digital cameras for the early detection of fungal attack on wood surfaces in different applications. However, the measurements based on chemical characterisation should be the next step to take in order significantly to enhance the model and increase the range of robust applications. In the current study, a multivariate model describing the influence of each fungal-covering area on mould grade was presented for the first time.

Place, publisher, year, edition, pages
MDPI, 2019
Keywords
bacteria, potassium silicate, N-Alkylbensyldimethylammonium chloride, wood, fungi, mould area, PLS modeling
National Category
Wood Science Other Mechanical Engineering
Research subject
Wood Science and Engineering
Identifiers
urn:nbn:se:ltu:diva-75696 (URN)10.3390/f10090714 (DOI)000487978700070 ()2-s2.0-85072556439 (Scopus ID)
Note

Validerad;2019;Nivå 2;2019-08-27 (johcin)

Available from: 2019-08-26 Created: 2019-08-26 Last updated: 2020-01-28Bibliographically approved
Xiong, S., Martín, C., Eilertsen, L., Wei, M., Myronycheva, O., Larsson, S., . . . Jönsson, L. (2019). Energy-efficient substrate pasteurisation for combined production of shiitake mushroom (Lentinula edodes) and bioethanol. Bioresource Technology, 274, 65-72
Open this publication in new window or tab >>Energy-efficient substrate pasteurisation for combined production of shiitake mushroom (Lentinula edodes) and bioethanol
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2019 (English)In: Bioresource Technology, ISSN 0960-8524, E-ISSN 1873-2976, Vol. 274, p. 65-72Article in journal (Refereed) Published
Abstract [en]

Hot-air (75 -100°C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield. Compared to autoclaving, HAP resulted in faster mycelial growth, earlier fructification, and higher or comparable fruit-body yield. The heat pretreatment methods did not differ regarding the fractions of carbohydrate and lignin in pretreated material and SMS, but HAP typically resulted in lower fractions of extractives. Shiitake cultivation, which reduced the mass fraction of lignin to less than half of the initial without having any major impact on the mass fraction of glucan, enhanced enzymatic hydrolysis of glucan about four-fold. The choice of heating method did not affect enzymatic digestibility. Thus, HAP could substitute autoclaving and facilitate combined shiitake mushroom and bioethanol production.

Place, publisher, year, edition, pages
Elsevier, 2019
Keywords
Edible fungi, Enzymatic digestibility, Hot-air pasteurisation, Lignin degradation, Spent mushroom substrate
National Category
Bioenergy Other Mechanical Engineering
Research subject
Wood Science and Engineering
Identifiers
urn:nbn:se:ltu:diva-71700 (URN)10.1016/j.biortech.2018.11.071 (DOI)000454610900009 ()30500765 (PubMedID)
Note

Validerad;2018;Nivå 2;2018-12-05 (svasva)

Available from: 2018-11-22 Created: 2018-11-22 Last updated: 2019-03-27Bibliographically approved
Sekan, A. S., Myronycheva, O., Karlsson, O., Gryganskyi, A. P. & Blume, Y. (2019). Green potential of Pleurotus spp. in biotechnology. PeerJ, 7, Article ID e6664.
Open this publication in new window or tab >>Green potential of Pleurotus spp. in biotechnology
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2019 (English)In: PeerJ, ISSN 2167-8359, E-ISSN 2167-8359, Vol. 7, article id e6664Article in journal (Refereed) Published
Abstract [en]

Background

The genus Pleurotus is most exploitable xylotrophic fungi, with valuable biotechnological, medical, and nutritional properties. The relevant features of the representatives of this genus to provide attractive low-cost industrial tools have been reported in numerous studies to resolve the pressure of ecological issues. Additionally, a number of Pleurotus species are highly adaptive, do not require any special conditions for growth, and possess specific resistance to contaminating diseases and pests. The unique properties of Pleurotus species widely used in many environmental technologies, such as organic solid waste recycling, chemical pollutant degradation, and bioethanol production.

Methodology

The literature study encompasses peer-reviewed journals identified by systematic searches of electronic databases such as Google Scholar, NCBI, Springer, ResearchGate, ScienceDirect, and ISI Web of Knowledge. The search scheme was divided into several steps, as described below.

Results

In this review, we describe studies examining the biotechnological feasibility of Pleurotus spp. to elucidate the importance of this genus for use in green technology. Here, we review areas of application of the genus Pleurotus as a prospective biotechnological tool.

Conclusion

The incomplete description of some fungal biochemical pathways emphasises the future research goals for this fungal culture.

Place, publisher, year, edition, pages
PeerJ, Inc, 2019
Keywords
Waste bioremediation, Mushroom cultivation, Pleurotus, Degradation, Polycyclic aromatic hydrocarbons, Enzyme, Lignin, Gene, Identification
National Category
Engineering and Technology Industrial Biotechnology Other Mechanical Engineering
Research subject
Wood Science and Engineering
Identifiers
urn:nbn:se:ltu:diva-73354 (URN)10.7717/peerj.6664 (DOI)000462697000007 ()30967974 (PubMedID)
Note

Validerad;2019;Nivå 2;2019-04-10 (inah)

Available from: 2019-03-29 Created: 2019-03-29 Last updated: 2019-04-12Bibliographically approved
Myronycheva, O., Karlsson, O., Sehlstedt-Persson, M., Öhman, M. & Sandberg, D. (2019). Portable microNIR sensor for the evaluation of mould contamination on wooden surfaces. In: LeVan-Green, S. (Ed.), Proceedings of the 62nd International Convention of ociety of Wood Science and Technology. Convention  Tenaya Lodge, Yosemite, California, USA, October 20-25: Theme: Renewable Materials and the Wood-based Bioeconomy,. Paper presented at 62nd International Convention of ociety of Wood Science and Technology (pp. 46-51). SWST, Society of Wood Science and Technology
Open this publication in new window or tab >>Portable microNIR sensor for the evaluation of mould contamination on wooden surfaces
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2019 (English)In: Proceedings of the 62nd International Convention of ociety of Wood Science and Technology. Convention  Tenaya Lodge, Yosemite, California, USA, October 20-25: Theme: Renewable Materials and the Wood-based Bioeconomy, / [ed] LeVan-Green, S., SWST, Society of Wood Science and Technology , 2019, p. 46-51Conference paper, Published paper (Refereed)
Place, publisher, year, edition, pages
SWST, Society of Wood Science and Technology, 2019
National Category
Wood Science
Research subject
Wood Science and Engineering
Identifiers
urn:nbn:se:ltu:diva-76467 (URN)978-0-9817876-9-5 (ISBN)
Conference
62nd International Convention of ociety of Wood Science and Technology
Available from: 2019-10-22 Created: 2019-10-22 Last updated: 2020-01-28
Myronycheva, O., Karlsson, O., Sehlstedt-Persson, M., Öhman, M. & Sandberg, D. (2018). Distribution of low-molecular lipophilic extractives beneath the surface of air- and kiln-dried Scots pine sapwood boards. PLoS ONE, 13(10), Article ID e0204212.
Open this publication in new window or tab >>Distribution of low-molecular lipophilic extractives beneath the surface of air- and kiln-dried Scots pine sapwood boards
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2018 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 13, no 10, article id e0204212Article in journal (Refereed) Published
Abstract [en]

During industrial wood drying, extractives migrate towards the wood surfaces and make the material more susceptible to photo/biodegradation. The present work provides information about the distribution, quantity and nature of lipophilic substances beneath the surface in air- and kiln-dried Scots pine (Pinus sylvestris L.) sapwood boards. Samples were taken from knot-free sapwood surfaces and the composition of lipophilic extractives, phenols and low-molecular fatty/resin acids layers at different nominal depths below the surface was studied gravimetrically, by UV-spectrometry and by gas chromatography-mass spectrometry (GC-MS). The concentration of total extractives was significantly higher in kiln-dried than in air-dried samples and was higher close to the surface than in the layers beneath. The scatter in the values for the lipophilic extractives was high in both drying types, being highest for linoleic acid and slightly lower for palmitic, oleic and stearic acids. The amount of fatty acids was low in kiln-dried boards, probably due to a stronger degradation due to the high temperature employed. The most abundant resin acid was dehydroabietic acid followed by pimaric, isopimaric, and abietic acids in both drying types. It is concluded that during kiln-drying a migration front is created at a depth of 0.25 mm with a thickness of about 0.5 mm.

Place, publisher, year, edition, pages
PLOS, 2018
National Category
Wood Science Other Mechanical Engineering
Research subject
Wood Science and Engineering
Identifiers
urn:nbn:se:ltu:diva-71200 (URN)10.1371/journal.pone.0204212 (DOI)000446921100033 ()30303988 (PubMedID)2-s2.0-85054716404 (Scopus ID)
Projects
Experimental studies of capillary phenomena in bio-based materialsFungal growth on modified wood-based products under subarctic conditionsModWoodLifeUnderstanding wood modification through an integrated scientific and environmental impact approach
Funder
Swedish Research Council
Note

Validerad;2018;Nivå 2;2018-10-29 (svasva)

Available from: 2018-10-13 Created: 2018-10-13 Last updated: 2020-01-28Bibliographically approved
Myronycheva, O., Sehlstedt-Persson, M., Karlsson, O. & Sandberg, D. (2018). Growth of mold and rot fungi on copper-impregnated Scots pine sapwood: Influence of planing depth and inoculation pattern.. BioResources, 13(4), 8787-8801
Open this publication in new window or tab >>Growth of mold and rot fungi on copper-impregnated Scots pine sapwood: Influence of planing depth and inoculation pattern.
2018 (English)In: BioResources, ISSN 1930-2126, E-ISSN 1930-2126, Vol. 13, no 4, p. 8787-8801Article in journal (Refereed) Published
Abstract [en]

The biocidal properties of an industrially used copper-based preservative were evaluated at different planing depths on exposure of pine wood to mold fungi in direct and indirect contamination methods, with simultaneous verification of white rot fungi virulence on wood. The preservative was an aqueous solution of copper carbonate, 2-aminoethanol, and quaternary ammonium compounds. Full cell preservative impregnation efficiency against visual mold fungi growth was tested on sapwood surfaces planed to different depths before impregnation. The virulence of two white rot fungal strains of Trametes versicolor (441 and JPEI) against the dried non-impregnated and impregnated wood samples was also tested. The unplaned surface of impregnated timber was occupied by air-borne contaminants, such as Paecilomyces variottiand Aspergillus niger up to 30%, and, even after impregnation it was necessary to process the surface to avoid micro-fungi settlement. The virulence of the tested rot fungi strains was confirmed by the aggressive degradation of non-impregnated wood with a mass loss of over 40%. Both Trametes sp. strains degraded the preservative-impregnated wood with a mass loss of 3.1% to 4.8%, but degradation by the JPEI strain was more intensive and more dependent on planing depth than the other strain (441).

Place, publisher, year, edition, pages
North Carolina State University, 2018
National Category
Wood Science Other Mechanical Engineering
Research subject
Wood Science and Engineering
Identifiers
urn:nbn:se:ltu:diva-71285 (URN)10.15376/biores.13.4.8787-8801 (DOI)000454215100118 ()2-s2.0-85056570471 (Scopus ID)
Note

Validerad;2018;Nivå 2;2018-10-30 (svasva)

Available from: 2018-10-19 Created: 2018-10-19 Last updated: 2020-01-28Bibliographically approved
Myronycheva, O., Karlsson, O., Sehlstedt-Persson, M., Hagman, O., Sidorova, E. & Sandberg, D. (2018). Hyperspectral Imaging Surface Analysis for Dried and Thermally Modified Wood: An Exploratory Study. Journal of Spectroscopy, Article ID 7423501.
Open this publication in new window or tab >>Hyperspectral Imaging Surface Analysis for Dried and Thermally Modified Wood: An Exploratory Study
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2018 (English)In: Journal of Spectroscopy, ISSN 2314-4920, E-ISSN 2314-4939, article id 7423501Article in journal (Refereed) Published
Abstract [en]

Naturally seasoned, kiln-dried, and thermally modified wood has been studied by hyperspectral near-infrared imaging between 980 and 2500 nm in order to obtain spatial chemical information. Evince software was used to explore, preprocess, and analyse spectral data from image pixels and link these data to chemical information via spectral wavelength assignment. A PCA model showed that regions with high absorbance were related to extractives with phenolic groups and aliphatic hydrocarbons. The sharp wavelength band at 2135 nm was found by multivariate analysis to be useful for multivariate calibration. This peak represents the largest variation that characterizes the knot area and can be related to areas in wood rich in hydrocarbons and phenol, and it can perhaps be used for future calibration of other wood surfaces. The discriminant analysis of thermally treated wood showed the strongest differentiation between the planed and rip-cut wood surfaces and a fairly clear discrimination between the two thermal processes. The wavelength band at 2100 nm showed the greatest difference and may correspond to stretching of C=O-O of polymeric acetyl groups, but this requires confirmation by chemical analysis.

Place, publisher, year, edition, pages
Hindawi Publishing Corporation, 2018
National Category
Wood Science
Research subject
Wood Science and Engineering
Identifiers
urn:nbn:se:ltu:diva-71584 (URN)10.1155/2018/7423501 (DOI)2-s2.0-85057402277 (Scopus ID)
Note

Validerad;2018;Nivå 2;2018-11-20 (svasva)

Available from: 2018-11-14 Created: 2018-11-14 Last updated: 2020-01-28Bibliographically approved
Myronycheva, O., Bandura, I., Bisko, N., Grygansky, A. . & Karlsson, O. (2017). Assessment of the Growth and Fruiting of 19 Oyster Mushroom Strains for Indoor Cultivation on Lignocellulosic Wastes. BioResources, 12(3), 4606-4626
Open this publication in new window or tab >>Assessment of the Growth and Fruiting of 19 Oyster Mushroom Strains for Indoor Cultivation on Lignocellulosic Wastes
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2017 (English)In: BioResources, ISSN 1930-2126, E-ISSN 1930-2126, Vol. 12, no 3, p. 4606-4626Article in journal (Refereed) Published
Abstract [en]

AbstractTwelve Pleurotus ostreatus (Jacq. ex Fr.) P. Kumm and six Pleurotus pulmonarius (Fr.) Quél. strains were characterized from the National Culture Collection of Mushrooms, Institute of Botany Kholodny, National Academy of Science, Kyiv, Ukraine (IBK). The strains were grown under commercial conditions on a mixture of wheat straw and sunflower shells under both winter and summer temperatures typical for those climatic conditions. The strains were divided into three groups according to their growing patterns. Important characteristics were compared with a commercial analogue, HK-35, such as vegetative growth, generative growth, and biological efficiency (1.9- to 3.1-fold), and were recorded for strains 2251, 2292, 2316, 2319, and 2320 of P. ostreatus and 2314 of P. pulmonarius. Strains 2251, 2292, 2301, 2321 and 2323 were the most suitable for commercial production, while strains 2319 and 2320 could satisfy processing industry requirements with their high biological efficiency. Strains 2287 and 2317 produced high-quality fruit bodies but probably required a higher temperature for cultivation. Strain 2318 might be attractive for some consumers due to its unique and unusual fruit body shape. Strain 2314 was the most promising for summer cultivation, while strain 537 produced the highest quality fruit bodies.KeywordsOyster mushroom; Strain; Vegetative growth; Fruit body; Biological efficiency; Conversion Factor (CF); Asymmetry of the Fruit Body Cap (Cas)

Place, publisher, year, edition, pages
North Carolina State University, 2017
National Category
Agricultural and Veterinary sciences Other Mechanical Engineering
Research subject
Wood Science and Engineering
Identifiers
urn:nbn:se:ltu:diva-63345 (URN)10.15376/biores.12.3.4606-4626 (DOI)000408497700008 ()2-s2.0-85026736554 (Scopus ID)
Note

Validerad; 2017; Nivå 2; 2017-05-17 (andbra)

Available from: 2017-05-13 Created: 2017-05-13 Last updated: 2018-07-10Bibliographically approved
Karlsson, O., Myronycheva, O., Sehlstedt-Persson, M., Öhman, M. & Sandberg, D. (2017). Multivariate modeling of mould growth in relation to extractives in dried Scots pine sapwood. In: Proceedings IRG Annual Meetin: . Paper presented at 48th Conference of the International Research Group on Wood Protection, IRG48, Ghent, Belgium, 4-8 Jun 2017. , Article ID 17-20629.
Open this publication in new window or tab >>Multivariate modeling of mould growth in relation to extractives in dried Scots pine sapwood
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2017 (English)In: Proceedings IRG Annual Meetin, 2017, article id 17-20629Conference paper, Poster (with or without abstract) (Refereed)
Abstract [en]

Influence of extractives on mould growth on Scots pine sapwood dried in air or in kiln was studied. Boards were sprayed with water mixtures of spores of the fungal species Penicillium sp., Aspergillus sp., Mucor sp., Paecilomyces sp., Trichoderma sp., treated at a temperature of 22ºC at 90% RH, classified into a percentage of covered area. Acetone and water extracts were isolated and analyzed for sugars, nitrogen, ash, resin/fatty acids, glycerol, and phenols. A multivariate Orthogonal Partial Least Squares (OPLS) regression model was developed to study relations between the extent of mould coverage of boards and chemical content. The model describes 51% variability in X and 69% in Y with prediction power of 55%. The results indicated that total acetone soluble extractives and sugars like glucose contributed to increased mould growth whereas fatty acids prevent mould growth.

Keywords
Multivariate analysis, sapwood, fungi
National Category
Materials Engineering Other Mechanical Engineering
Research subject
Wood Science and Engineering
Identifiers
urn:nbn:se:ltu:diva-63985 (URN)
Conference
48th Conference of the International Research Group on Wood Protection, IRG48, Ghent, Belgium, 4-8 Jun 2017
Available from: 2017-06-14 Created: 2017-06-14 Last updated: 2020-01-28Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0001-7864-8091

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