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Publications (10 of 46) Show all publications
Phongpreecha, T., Liu, J., Hodge, D. & Qi, Y. (2019). Adsorption of Lignin β-O-4 Dimers on Metal Surfaces in Vacuum and Solvated Environments. ACS Sustainable Chemistry and Engineering (2), 2667-2678
Open this publication in new window or tab >>Adsorption of Lignin β-O-4 Dimers on Metal Surfaces in Vacuum and Solvated Environments
2019 (English)In: ACS Sustainable Chemistry and Engineering, ISSN 2168-0485, no 2, p. 2667-2678Article in journal (Refereed) Published
Abstract [en]

Lignin hydrogenolysis has recently been studied extensively as it was shown to result in high monomer yields. Most of these reactions were conducted in liquid solvents, which have shown large impacts on product types and yields. Because adsorption is the first step to any heterogeneous catalyst reactions, this work aims to understand how solvent affects lignin adsorption on Ni(111) and Cu(111) surfaces. To achieve this, density functional theory calculations were employed to investigate β-O-4 lignin dimer (a model compound) adsorption conformations in both vacuum and liquid ethanol. In vacuum, it was found that lignin prefers to adsorb strongly on Ni(111) and weakly on Cu(111) with both aromatic rings parallel to the surface. Solvated adsorption was modeled using both implicit and explicit models. It was found that an explicit model is required to accurately describe the lignin-solvent interactions. With the explicit solvation model, it was predicted that the lignin dimer adsorbs on a Ni(111) surface but not on Cu(111). Furthermore, to circumvent the computationally expensive liquid interface calculations, a thermodynamic cycle method was developed to quickly estimate the solvated lignin dimer adsorption energy from the gas phase adsorption energy and the solvation energies. This model quantifies the effects from the solvent on lignin dimer adsorption, including the contributions from the lignin-solvent and the solvent-metal interactions, and suggests how to design both catalyst and solvent to tune lignin adsorption. 

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2019
Keywords
adsorption energy, lignin valorization, lignocellulosic biomass, solvation model
National Category
Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
urn:nbn:se:ltu:diva-72830 (URN)10.1021/acssuschemeng.8b05736 (DOI)000456631800092 ()2-s2.0-85060381279 (Scopus ID)
Note

Validerad;2019;Nivå 2;2019-02-08 (svasva)

Available from: 2019-02-08 Created: 2019-02-08 Last updated: 2019-02-08Bibliographically approved
Singh, S. K., Savoy, A. W., Yuan, Z., Luo, H., Stahl, S. S., Hegg, E. L. & Hodge, D. (2019). Integrated Two-Stage Alkaline-Oxidative Pretreatment of Hybrid Poplar. Part 1: Impact of Alkaline Pre-Extraction Conditions on Process Performance and Lignin Properties. Industrial & Engineering Chemistry Research, 58(35), 15989-15999
Open this publication in new window or tab >>Integrated Two-Stage Alkaline-Oxidative Pretreatment of Hybrid Poplar. Part 1: Impact of Alkaline Pre-Extraction Conditions on Process Performance and Lignin Properties
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2019 (English)In: Industrial & Engineering Chemistry Research, ISSN 0888-5885, E-ISSN 1520-5045, Vol. 58, no 35, p. 15989-15999Article in journal (Refereed) Published
Abstract [en]

We previously demonstrated that a two-stage pretreatment comprising of an alkaline pre-extraction followed by a Cu-catalyzed alkaline–oxidative treatment is effective at pretreating hardwoods under relatively mild reaction conditions. In this work, we focus on characterizing how biomass source and reaction conditions used during the alkaline pre-extraction impact the subsequent processing stages as well as lignin yields and properties. Specifically, three hybrid poplars were subjected to the first stage alkaline pre-extraction under various conditions including differences in time (15–300 min), temperature (95–155 °C), and alkali loadings (50–200 mg NaOH/g biomass), and the impact on total mass solubilization, lignin recovery, and lignin purity was determined. Empirical correlations were developed between reaction conditions and mass solubilization and lignin recovery during the pre-extraction stage. For select conditions, lignin properties were assessed and include β-O-4 content determined by 13C NMR, molecular mass distributions as determined by gel permeation chromatography, and susceptibility to depolymerization to aromatic monomers using thioacidolysis and formic acid catalyzed depolymerization. We found alkaline pre-extraction performed at higher temperatures generated lignins exhibiting lower contamination by polysaccharides, lower aromatic monomer yields from depolymerization, lower molar masses, and lower β-O-4 contents relative to the lower temperature pre-extractions. Finally, the pre-extracted biomass from select conditions was assessed for its response to the subsequent Cu-catalyzed alkaline–oxidative treatment and enzymatic hydrolysis. It was demonstrated that minor differences in delignification during pre-extraction have quantifiable impacts on the subsequent efficacy of the second stage of pretreatment and enzymatic hydrolysis with improved lignin removal during the first pre-extraction stage resulting in improved hydrolysis yields.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2019
National Category
Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
urn:nbn:se:ltu:diva-76145 (URN)10.1021/acs.iecr.9b01124 (DOI)000484827100019 ()
Note

Validerad;2019;Nivå 2;2019-09-27 (johcin)

Available from: 2019-09-27 Created: 2019-09-27 Last updated: 2019-09-27Bibliographically approved
Yuan, Z., Singh, S. K., Bals, B., Hodge, D. B. & Hegg, E. L. (2019). Integrated Two-Stage Alkaline–Oxidative Pretreatment of Hybrid Poplar. Part 2: Impact of Cu-Catalyzed Alkaline Hydrogen Peroxide Pretreatment Conditions on Process Performance and Economics. Industrial & Engineering Chemistry Research, 58(35), 16000-16008
Open this publication in new window or tab >>Integrated Two-Stage Alkaline–Oxidative Pretreatment of Hybrid Poplar. Part 2: Impact of Cu-Catalyzed Alkaline Hydrogen Peroxide Pretreatment Conditions on Process Performance and Economics
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2019 (English)In: Industrial & Engineering Chemistry Research, ISSN 0888-5885, E-ISSN 1520-5045, Vol. 58, no 35, p. 16000-16008Article in journal (Refereed) Published
Abstract [en]

Two-stage alkaline/copper 2,2′-bipyridine-catalyzed alkaline hydrogen peroxide (Cu-AHP) pretreatment is an effective strategy for improving the enzymatic digestibility of hybrid poplar. To reduce the chemical inputs and processing costs associated with this process, we investigated the effect of increasing the temperature for both the alkaline pre-extraction and the Cu-AHP pretreatment stages. The results indicate that increasing the alkaline pre-extraction and the Cu-AHP pretreatment temperatures from 30 to 120 and 80 °C, respectively, allowed us to reduce both the pretreatment time of the Cu-AHP stage and the chemical loadings. Incubating alkaline pre-extracted hybrid poplar for 12 h with 10% NaOH (w/w biomass), 8% hydrogen peroxide (w/w biomass), and a Cu2+ and 2,2′-bipyridine (bpy) concentration of 1 mM yielded monomeric sugar yields of approximately 77% glucose and 66% xylose (based on the initial sugar composition) following enzymatic hydrolysis. Technoeconomic analysis (TEA) indicates that these changes to the two-stage alkaline/Cu-AHP pretreatment process could potentially reduce the minimum fuel selling price (MFSP) by more than $1.00 per gallon of biofuel compared to the reference case where both stages were conducted at 30 °C with higher chemical inputs.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2019
National Category
Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
urn:nbn:se:ltu:diva-76144 (URN)10.1021/acs.iecr.9b00901 (DOI)000484827100020 ()
Note

Validerad;2019;Nivå 2;2019-09-27 (johcin)

Available from: 2019-09-27 Created: 2019-09-27 Last updated: 2019-09-27Bibliographically approved
Williams, D. L., Ong, R. G., Mullet, J. E. & Hodge, D. (2019). Integration of Pretreatment With Simultaneous Counter-Current Extraction of Energy Sorghum for High-Titer Mixed Sugar Production. Frontiers in Energy Research, 6, Article ID 133.
Open this publication in new window or tab >>Integration of Pretreatment With Simultaneous Counter-Current Extraction of Energy Sorghum for High-Titer Mixed Sugar Production
2019 (English)In: Frontiers in Energy Research, E-ISSN 2296-598X, Vol. 6, article id 133Article in journal (Refereed) Published
Abstract [en]

Sorghum (Sorghum bicolor L. Moench) offers substantial potential as a feedstock for the production of sugar-derived biofuels and biochemical products from cell wall polysaccharides (i.e., cellulose and hemicelluloses) and water-extractable sugars (i.e., glucose, fructose, sucrose, and starch). A number of preprocessing schemes can be envisioned that involve processes such as sugar extraction, pretreatment, and densification that could be employed in decentralized, regional-scale biomass processing depots. In this work, an energy sorghum exhibiting a combination of high biomass productivity and high sugar accumulation was evaluated for its potential for integration into several potential biomass preprocessing schemes. This included counter-current extraction of water-soluble sugars followed bymild NaOH or liquid hot water pretreatment of the extracted bagasse. A novel processing scheme was investigated that could integrate with current diffuser-type extraction systems for sugar extraction. In this approach, mild NaOH pretreatment (i.e., < 90 degrees C) was performed as a counter-current extraction to yield both an extracted, pretreated bagasse and a high-concentration mixed sugar stream. Following hydrolysis of the bagasse, the combined hydrolysates derived from cellulosic sugars and extractable sugars were demonstrated to be fermentable to high ethanol titers (> 8%) at high metabolic yields without detoxification using a Saccharomyces cerevisiae strain metabolically engineered and evolved to ferment xylose.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2019
Keywords
sorghum, sucrose extraction, decentralized biorefining, pretreatment, cellulosic biofuels
National Category
Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
urn:nbn:se:ltu:diva-74648 (URN)10.3389/fenrg.2018.00133 (DOI)000467003700001 ()
Note

Validerad;2019;Nivå 2;2019-06-18 (johcin)

Available from: 2019-06-18 Created: 2019-06-18 Last updated: 2019-07-02Bibliographically approved
Alinejad, M., Henry, C., Nikafshar, S., Gondaliya, A., Bagheri, B., Chen, N., . . . Nejad, M. (2019). Lignin-based polyurethanes: Opportunities for bio-based foams, elastomers, coatings and adhesives. Polymers, 11(7), Article ID 1202.
Open this publication in new window or tab >>Lignin-based polyurethanes: Opportunities for bio-based foams, elastomers, coatings and adhesives
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2019 (English)In: Polymers, ISSN 2073-4360, E-ISSN 2073-4360, Vol. 11, no 7, article id 1202Article in journal (Refereed) Published
Abstract [en]

Polyurethane chemistry can yield diverse sets of polymeric materials exhibiting a widerange of properties for various applications and market segments. Utilizing lignin as a polyol presentsan opportunity to incorporate a currently underutilized renewable aromatic polymer into theseproducts. In this work, we will review the current state of technology for utilizing lignin as a polyolreplacement in different polyurethane products. This will include a discussion of lignin structure,diversity, and modification during chemical pulping and cellulosic biofuels processes, approachesfor lignin extraction, recovery, fractionation, and modification/functionalization. We will discussthe potential of incorporation of lignins into polyurethane products that include rigid and flexiblefoams, adhesives, coatings, and elastomers. Finally, we will discuss challenges in incorporating ligninin polyurethane formulations, potential solutions and approaches that have been taken to resolvethose issues.

Place, publisher, year, edition, pages
MDPI, 2019
Keywords
lignin, polyurethane, adhesives, foams, coatings, elastomers
National Category
Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
urn:nbn:se:ltu:diva-75608 (URN)10.3390/polym11071202 (DOI)000480539500119 ()31323816 (PubMedID)2-s2.0-85070386828 (Scopus ID)
Note

Validerad;2019;Nivå 2;2019-08-20 (svasva)

Available from: 2019-08-20 Created: 2019-08-20 Last updated: 2019-08-30Bibliographically approved
Bhalla, A., Cai, C. M., Xu, F., Singh, S. K., Bansal, N., Phongpreecha, T., . . . Hodge, D. (2019). Performance of three delignifying pretreatments on hardwoods: hydrolysis yields, comprehensive mass balances, and lignin properties. Biotechnology for Biofuels, 12, Article ID 213.
Open this publication in new window or tab >>Performance of three delignifying pretreatments on hardwoods: hydrolysis yields, comprehensive mass balances, and lignin properties
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2019 (English)In: Biotechnology for Biofuels, ISSN 1754-6834, E-ISSN 1754-6834, Vol. 12, article id 213Article in journal (Refereed) Published
Abstract [en]

Background

In this work, three pretreatments under investigation at the DOE Bioenergy Research Centers (BRCs) were subjected to a side-by-side comparison to assess their performance on model bioenergy hardwoods (a eucalyptus and a hybrid poplar). These include co-solvent-enhanced lignocellulosic fractionation (CELF), pretreatment with an ionic liquid using potentially biomass-derived components (cholinium lysinate or [Ch][Lys]), and two-stage Cu-catalyzed alkaline hydrogen peroxide pretreatment (Cu-AHP). For each of the feedstocks, the pretreatments were assessed for their impact on lignin and xylan solubilization and enzymatic hydrolysis yields as a function of enzyme loading. Lignins recovered from the pretreatments were characterized for polysaccharide content, molar mass distributions, β-aryl ether content, and response to depolymerization by thioacidolysis.

Results

All three pretreatments resulted in significant solubilization of lignin and xylan, with the CELF pretreatment solubilizing the majority of both biopolymer categories. Enzymatic hydrolysis yields were shown to exhibit a strong, positive correlation with the lignin solubilized for the low enzyme loadings. The pretreatment-derived solubles in the [Ch][Lys]-pretreated biomass were presumed to contribute to inhibition of enzymatic hydrolysis in the eucalyptus as a substantial fraction of the pretreatment liquor was carried forward into hydrolysis for this pretreatment. The pretreatment-solubilized lignins exhibited significant differences in polysaccharide content, molar mass distributions, aromatic monomer yield by thioacidolysis, and β-aryl ether content. Key trends include a substantially higher polysaccharide content in the lignins recovered from the [Ch][Lys] pretreatment and high β-aryl ether contents and aromatic monomer yields from the Cu-AHP pretreatment. For all lignins, the 13C NMR-determined β-aryl ether content was shown to be correlated with the monomer yield with a second-order functionality.

Conclusions

Overall, it was demonstrated that the three pretreatments highlighted in this study demonstrated uniquely different functionalities in reducing biomass recalcitrance and achieving higher enzymatic hydrolysis yields for the hybrid poplar while yielding a lignin-rich stream that may be suitable for valorization. Furthermore, modification of lignin during pretreatment, particularly cleavage of β-aryl ether bonds, is shown to be detrimental to subsequent depolymerization.

Place, publisher, year, edition, pages
BioMed Central, 2019
Keywords
Pretreatment, Cellulosic biofuels, Lignin, Aromatic monomers
National Category
Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
urn:nbn:se:ltu:diva-76112 (URN)10.1186/s13068-019-1546-0 (DOI)000484953600003 ()31516552 (PubMedID)
Note

Validerad;2019;Nivå 2;2019-09-25 (johcin)

Available from: 2019-09-25 Created: 2019-09-25 Last updated: 2019-09-27Bibliographically approved
Li, M., Yan, G., Bhalla, A., Maldonado-Pereira, L., Russell, P. R., Ding, S.-Y., . . . Hodge, D. (2018). Physical fractionation of sweet sorghum and forage/energy sorghum for optimal processing in a biorefinery. Industrial crops and products (Print), 124, 607-616
Open this publication in new window or tab >>Physical fractionation of sweet sorghum and forage/energy sorghum for optimal processing in a biorefinery
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2018 (English)In: Industrial crops and products (Print), ISSN 0926-6690, E-ISSN 1872-633X, Vol. 124, p. 607-616Article in journal (Refereed) Published
Abstract [en]

Sorghum offers enormous potential as a feedstock for the production of fuels and chemicals from both water-extractable sugars and the cell wall biopolymers, while its within-plant structural and compositional heterogeneity may allow for physical fractionations to tailor feedstock properties to a biorefining process. In this study, the stem internodes of two sorghum (Sorghum bicolor L. Moench) genotypes, a sweet sorghum (‘Della’) and a forage/energy sorghum (‘TX08001’), were first subjected to fractionation by manual classification by stem anatomy and internode proximity to the ground to yield 18 fractions. These fractions exhibited substantial differences in cell wall morphology, composition, and recalcitrance to mild alkaline pretreatment and enzymatic hydrolysis. While the sweet sorghum cultivar held nearly 70% more water-extractable sugar (sucrose, glucose, fructose, starch) in the stems than the forage/energy sorghum hybrid, both cultivars exhibited comparable diversity of composition and these compositions were remarkably similar in similar tissues and stem regions between the two cultivars. The fractions isolated from the pith parenchyma were the least recalcitrant to mild alkaline pretreatment and enzymatic hydrolysis and contained less lignin than fractions isolated from the epidermis, outer and inner rind, and internal vascular bundles. The pith samples isolated from the lowest region of the stem from both cultivars exhibited near-theoretical sugar hydrolysis yields when no pretreatment was employed and exhibited the lowest lignin contents of any of the fractions. Next, a physical fractionation approach approximating a commercial “de-pithing” process utilizing wet disintegration and sieving was applied to the forage/energy sorghum. A pith-rich fraction representing approximately 20% of the extractives-free mass of the stem could be isolated with this approach and, relative to the other fractions, was low in lignin, high in ash, highly hygroscopic, and showed an improved response to mild alkaline pretreatment and enzymatic hydrolysis at low enzyme loadings. Overall, these results demonstrate how heterogeneity within sorghum stems can be exploited using physical fractionation approaches to yield fractions enriched in desired properties that may allow for more streamlined processing.

Place, publisher, year, edition, pages
Elsevier, 2018
National Category
Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
urn:nbn:se:ltu:diva-70621 (URN)10.1016/j.indcrop.2018.07.002 (DOI)000447569100070 ()2-s2.0-85051655727 (Scopus ID)
Note

Validerad;2018;Nivå 2;2018-08-28 (andbra)

Available from: 2018-08-28 Created: 2018-08-28 Last updated: 2018-11-15Bibliographically approved
Crowe, J. D., Feringa, N., Pattathil, S., Merritt, B. B., Foster, C. E., Dines, D., . . . Hodge, D. (2017). Identification of developmental stage and anatomical fraction contributions to cell wall recalcitrance in switchgrass. Biotechnology for Biofuels, 10(1), Article ID 184.
Open this publication in new window or tab >>Identification of developmental stage and anatomical fraction contributions to cell wall recalcitrance in switchgrass
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2017 (English)In: Biotechnology for Biofuels, ISSN 1754-6834, E-ISSN 1754-6834, Vol. 10, no 1, article id 184Article in journal (Refereed) Published
Abstract [en]

Background: Heterogeneity within herbaceous biomass can present important challenges for processing feedstocks to cellulosic biofuels. Alterations to cell wall composition and organization during plant growth represent major contributions to heterogeneity within a single species or cultivar. To address this challenge, the focus of this study was to characterize the relationship between composition and properties of the plant cell wall and cell wall response to deconstruction by NaOH pretreatment and enzymatic hydrolysis for anatomical fractions (stem internodes, leaf sheaths, and leaf blades) within switchgrass at various tissue maturities as assessed by differing internode. Results: Substantial differences in both cell wall composition and response to deconstruction were observed as a function of anatomical fraction and tissue maturity. Notably, lignin content increased with tissue maturity concurrently with decreasing ferulate content across all three anatomical fractions. Stem internodes exhibited the highest lignin content as well as the lowest hydrolysis yields, which were inversely correlated to lignin content. Confocal microscopy was used to demonstrate that removal of cell wall aromatics (i.e., lignins and hydroxycinnamates) by NaOH pretreatment was non-uniform across diverse cell types. Non-cellulosic polysaccharides were linked to differences in cell wall response to deconstruction in lower lignin fractions. Specifically, leaf sheath and leaf blade were found to have higher contents of substituted glucuronoarabinoxylans and pectic polysaccharides. Glycome profiling demonstrated that xylan and pectic polysaccharide extractability varied with stem internode maturity, with more mature internodes requiring harsher chemical extractions to remove comparable glycan abundances relative to less mature internodes. While enzymatic hydrolysis was performed on extractives-free biomass, extractible sugars (i.e., starch and sucrose) comprised a significant portion of total dry weight particularly in stem internodes, and may provide an opportunity for recovery during processing. Conclusions: Cell wall structural differences within a single plant can play a significant role in feedstock properties and have the potential to be exploited for improving biomass processability during a biorefining process. The results from this work demonstrate that cell wall lignin content, while generally exhibiting a negative correlation with enzymatic hydrolysis yields, is not the sole contributor to cell wall recalcitrance across diverse anatomical fractions within switchgrass

Place, publisher, year, edition, pages
BioMed Central, 2017
National Category
Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
urn:nbn:se:ltu:diva-65057 (URN)10.1186/s13068-017-0870-5 (DOI)000405470700001 ()28725264 (PubMedID)2-s2.0-85024897919 (Scopus ID)
Note

Validerad; 2017; Nivå 2; 2017-08-14 (andbra)

Available from: 2017-08-14 Created: 2017-08-14 Last updated: 2018-07-10Bibliographically approved
Phongpreecha, T., Hool, N. C., Stoklosa, R. J., Klett, A. S., Foster, C. E., Bhalla, A., . . . Hodge, D. B. (2017). Predicting lignin depolymerization yields from quantifiable properties using fractionated biorefinery lignins. Green Chemistry, 19(21), 5131-5143
Open this publication in new window or tab >>Predicting lignin depolymerization yields from quantifiable properties using fractionated biorefinery lignins
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2017 (English)In: Green Chemistry, ISSN 1463-9262, E-ISSN 1463-9270, Vol. 19, no 21, p. 5131-5143Article in journal (Refereed) Published
Abstract [en]

Lignin depolymerization to aromatic monomers with high yields and selectivity is essential for the economic feasibility of many lignin-valorization strategies within integrated biorefining processes. Importantly, the quality and properties of the lignin source play an essential role in impacting the conversion chemistry, yet this relationship between lignin properties and lignin susceptibility to depolymerization is not well established. In this study, we quantitatively demonstrate how the detrimental effect of a pretreatment process on the properties of lignins, particularly β-O-4 content, limit high yields of aromatic monomers using three lignin depolymerization approaches: thioacidolysis, hydrogenolysis, and oxidation. Through pH-based fractionation of alkali-solubilized lignin from hybrid poplar, this study demonstrates that the properties of lignin, namely β-O-4 linkages, phenolic hydroxyl groups, molecular weight, and S/G ratios exhibit strong correlations with each other even after pretreatment. Furthermore, the differences in these properties lead to discernible trends in aromatic monomer yields using the three depolymerization techniques. Based on the interdependency of alkali lignin properties and its susceptibility to depolymerization, a model for the prediction of monomer yields was developed and validated for depolymerization by quantitative thioacidolysis. These results highlight the importance of the lignin properties for their suitability for an ether-cleaving depolymerization process, since the theoretical monomer yields grows as a second order function of the β-O-4 content. Therefore, this research encourages and provides a reference tool for future studies to identify new methods for lignin-first biomass pretreatment and lignin valorization that emphasize preservation of lignin qualities, apart from focusing on optimization of reaction conditions and catalyst selection.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2017
National Category
Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
urn:nbn:se:ltu:diva-66227 (URN)10.1039/C7GC02023F (DOI)000414147100014 ()2-s2.0-85032741346 (Scopus ID)
Note

Validerad;2017;Nivå 2;2017-11-14 (andbra)

Available from: 2017-10-23 Created: 2017-10-23 Last updated: 2018-04-19Bibliographically approved
Li, M., Williams, D. L., Heckwolf, M., de Leon, N., Kaeppler, S., Sykes, R. W. & Hodge, D. (2017). Prediction of Cell Wall Properties and Response to Deconstruction Using Alkaline Pretreatment in Diverse Maize Genotypes Using Py-MBMS and NIR. Bioenergy Research, 10(2), 329-343
Open this publication in new window or tab >>Prediction of Cell Wall Properties and Response to Deconstruction Using Alkaline Pretreatment in Diverse Maize Genotypes Using Py-MBMS and NIR
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2017 (English)In: Bioenergy Research, ISSN 1939-1234, E-ISSN 1939-1242, Vol. 10, no 2, p. 329-343Article in journal (Refereed) Published
Abstract [en]

In this work, we explore the ability of several characterization approaches for phenotyping to extract information about plant cell wall properties in diverse maize genotypes with the goal of identifying approaches that could be used to predict the plant’s response to deconstruction in a biomass-to-biofuel process. Specifically, a maize diversity panel was subjected to two high-throughput biomass characterization approaches, pyrolysis molecular beam mass spectrometry (py-MBMS) and near-infrared (NIR) spectroscopy, and chemometric models to predict a number of plant cell wall properties as well as enzymatic hydrolysis yields of glucose following either no pretreatment or with mild alkaline pretreatment. These were compared to multiple linear regression (MLR) models developed from quantified properties. We were able to demonstrate that direct correlations to specific mass spectrometry ions from pyrolysis as well as characteristic regions of the second derivative of the NIR spectrum regions were comparable in their predictive capability to partial least squares (PLS) models for p-coumarate content, while the direct correlation to the spectral data was superior to the PLS for Klason lignin content and guaiacyl monomer release by thioacidolysis as assessed by cross-validation. The PLS models for prediction of hydrolysis yields using either py-MBMS or NIR spectra were superior to MLR models based on quantified properties for unpretreated biomass. However, the PLS models using the two high-throughput characterization approaches could not predict hydrolysis following alkaline pretreatment while MLR models based on quantified properties could. This is likely a consequence of quantified properties including some assessments of pretreated biomass, while the py-MBMS and NIR only utilized untreated biomass.

Place, publisher, year, edition, pages
Springer, 2017
National Category
Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
urn:nbn:se:ltu:diva-60047 (URN)10.1007/s12155-016-9798-z (DOI)000400861200003 ()
Note

Validerad; 2017; Nivå 2; 2017-05-12 (andbra)

Available from: 2016-10-31 Created: 2016-10-31 Last updated: 2018-11-15Bibliographically approved
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ORCID iD: ORCID iD iconorcid.org/0000-0002-9313-941x

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