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Synergy Effect of Nanocrystalline Cellulose for the Biosensing Detection of Glucose
School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi.
University Putra Malaysia, Department of Chemistry, Faculty of Science, University Putra Malaysia.
Luleå tekniska universitet, Institutionen för teknikvetenskap och matematik, Materialvetenskap.ORCID-id: 0000-0001-8909-3554
Department of Wood and Paper Science and Technology, Faculty of Natural Resources, University of Tehran.
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2015 (engelsk)Inngår i: Sensors, ISSN 1424-8220, E-ISSN 1424-8220, Vol. 15, nr 10, s. 24681-24697Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Integrating polypyrrole-cellulose nanocrystal-based composites with glucose oxidase (GOx) as a new sensing regime was investigated. Polypyrrole-cellulose nanocrystal (PPy-CNC)-based composite as a novel immobilization membrane with unique physicochemical properties was found to enhance biosensor performance. Field emission scanning electron microscopy (FESEM) images showed that fibers were nanosized and porous, which is appropriate for accommodating enzymes and increasing electron transfer kinetics. The voltammetric results showed that the native structure and biocatalytic activity of GOx immobilized on the PPy-CNC nanocomposite remained and exhibited a high sensitivity (ca. 0.73 μA·mM(-1)), with a high dynamic response ranging from 1.0 to 20 mM glucose. The modified glucose biosensor exhibits a limit of detection (LOD) of (50 ± 10) µM and also excludes interfering species, such as ascorbic acid, uric acid, and cholesterol, which makes this sensor suitable for glucose determination in real samples. This sensor displays an acceptable reproducibility and stability over time. The current response was maintained over 95% of the initial value after 17 days, and the current difference measurement obtained using different electrodes provided a relative standard deviation (RSD) of 4.47%.

sted, utgiver, år, opplag, sider
2015. Vol. 15, nr 10, s. 24681-24697
HSV kategori
Forskningsprogram
Trä och bionanokompositer
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URN: urn:nbn:se:ltu:diva-13299DOI: 10.3390/s151024681ISI: 000364242300001PubMedID: 26404269Scopus ID: 2-s2.0-84942808620Lokal ID: c7e25c12-8cb7-41b7-8086-64068171aaf1OAI: oai:DiVA.org:ltu-13299DiVA, id: diva2:986252
Merknad

Validerad; 2015; Nivå 2; 20150928 (andbra)

Tilgjengelig fra: 2016-09-29 Laget: 2016-09-29 Sist oppdatert: 2018-07-10bibliografisk kontrollert

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