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Valorization of Olive Mill Wastewater for the Production of β-glucans from Selected Basidiomycetes
Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens .ORCID-id: 0000-0003-0361-7690
Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens .
Luleå tekniska universitet, Institutionen för samhällsbyggnad och naturresurser, Kemiteknik.ORCID-id: 0000-0003-0079-5950
National Technical University of Athens, School of Chemical Engineering, National Technical University of Athens, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens.
Antal upphovsmän: 42017 (Engelska)Ingår i: Waste and Biomass Valorization, ISSN 1877-2641, E-ISSN 1877-265X, Vol. 8, nr 5, s. 1721-1731Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Purpose

The aim of the present study was to investigate the feasibility of polysaccharides production by selected basidiomycetes in submerged culture. Olive mill wastewater (OMWW) was also tested as a potential substrate for polysaccharides production by mushroom strains, focusing on the simultaneous degradation and valorization of the waste material.

Methods

The tested strains were grown in two different substrates, and after biomass harvesting, polysaccharides were isolated using two different methods. The extracellular polysaccharides were isolated from the culture broth, with ethanol precipitation. The isolated fractions were partially characterized with FT-IR spectroscopy.

Results

All three strains performed well in both substrates. Maximum degradation performance of OMWW was achieved by Ganoderma lucidum, achieving 19.4% phenols reduction together with 47.56% decolorization. The extracellular polysaccharides (EPS) produced by all strains were found to be richer in total glucans during growth in semi-synthetic medium, compared to growth in OMWW-based medium. In regard to biomass polysaccharides, Pleurotus ostreatus biomass was found to be richer in glucans, reaching 8.68% (w/w) total glucan content when grown in semi-synthetic medium and 7.58% (w/w) when grown in OMWW-based medium. After purification of biomass polysaccharides with two methods, the fraction with the highest glucan content was found to be the one from G. lucidum after growth in semi-synthetic medium cultures, with 49.1% (w/w) total glucans. FT-IR spectra of the isolated samples revealed the bands corresponding to α- and β-glucosidic bonds, but also the existence of protein contamination.

Conclusions

Purification of biomass polysaccharides with two distinct methods revealed that α-amylase and Sevag treatments failed to remove completely α-glucans and proteins respectively, leading to the suggestion that these two steps could be omitted without significant impact. Moreover, the results imply that the valorization of OMWW might be feasible with the use of mushroom strains, leading to the production of important products, such as glucans.

Ort, förlag, år, upplaga, sidor
Springer, 2017. Vol. 8, nr 5, s. 1721-1731
Nationell ämneskategori
Bioprocessteknik
Forskningsämne
Biokemisk processteknik
Identifikatorer
URN: urn:nbn:se:ltu:diva-62070DOI: 10.1007/s12649-017-9839-7ISI: 000406399700029Scopus ID: 2-s2.0-85011663073OAI: oai:DiVA.org:ltu-62070DiVA, id: diva2:1075206
Konferens
9th International Conference on the Environmental and Technical Implications of Construction with Alternative Materials (WASCON) - Resource Efficiency in Construction, Santander, Spain, Jun 10-12, 2015
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Tillgänglig från: 2017-02-17 Skapad: 2017-02-17 Senast uppdaterad: 2018-11-15Bibliografiskt granskad

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Zerva, AnastasiaChristakopoulos, Paul

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