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Development of microfluidic system and optical tweezers for electrophysiological investigations of an individual cell
Luleå University of Technology, Department of Computer Science, Electrical and Space Engineering, Signals and Systems.
Luleå University of Technology, Department of Computer Science, Electrical and Space Engineering, Signals and Systems.
2010 (English)In: Optical Trapping and Optical Micromanipulation VII: 1 - 5 August 2010, San Diego, California, United States ; [part of SPIE optics + photonics] / [ed] Kishan Dholakia; Gabriel C. Spalding, Bellingham, Wash: SPIE - International Society for Optical Engineering, 2010Conference paper, Published paper (Refereed)
Abstract [en]

We present a new approach of combining Lab-on-a-chip technologies with optical manipulation technique for accurate investigations in the field of cell biology. A general concept was to develop and combine different methods to perform advanced electrophysiological investigations of an individual living cell under optimal control of the surrounding environment. The conventional patch clamp technique was customized by modifying the open system with a gas-tight multifunctional microfluidics system and optical trapping technique (optical tweezers).The system offers possibilities to measure the electrical signaling and activity of the neuron under optimum conditions of hypoxia and anoxia while the oxygenation state is controlled optically by means of a spectroscopic technique. A cellbased microfluidics system with an integrated patch clamp pipette was developed successfully. Selectively, an individual neuron is manipulated within the microchannels of the microfluidic system under a sufficient control of the environment. Experiments were performed to manipulate single yeast cell and red blood cell (RBC) optically through the microfluidics system toward an integrated patch clamp pipette. An absorption spectrum of a single RCB was recorded which showed that laser light did not impinge on the spectroscopic spectrum of light. This is promising for further development of a complete lab-on-a-chip system for patch clamp measurements.

Place, publisher, year, edition, pages
Bellingham, Wash: SPIE - International Society for Optical Engineering, 2010.
Series
Proceedings of SPIE, the International Society for Optical Engineering, ISSN 0277-786X ; 7762
National Category
Other Medical Engineering
Research subject
Medical Engineering for Healthcare
Identifiers
URN: urn:nbn:se:ltu:diva-30364DOI: 10.1117/12.860478ISI: 000285837400055Scopus ID: 77958122661Local ID: 42466e60-b599-11df-a707-000ea68e967bISBN: 978-0-8194-8258-7 (print)OAI: oai:DiVA.org:ltu-30364DiVA, id: diva2:1003591
Conference
SPIE optics + Photonics : 01/08/2010 - 05/08/2010
Note
Validerad; 2010; 20110310 (ysko)Available from: 2016-09-30 Created: 2016-09-30 Last updated: 2018-07-10Bibliographically approved

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Alrifaiy, AhmedBitaraf, NazaninLindahl, OlofRamser, Kerstin

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