Turbulent pulsating flow in a 100 mm diameter pipe has been studied experimentally at a Reynolds number of 14,500. The work covers four different flow conditions; steady, pulsating flow at oscillation frequencies 0.08 and 0.4 Hz, and pulsating flow with simultaneous imposition of both frequencies. The amplitudes of the pulsations were 10 and 7.5% of the bulk flow at 0.08 and 0.4 Hz, respectively. Laser Doppler anemometry, hot-film and pressure measurements show that the mean values of velocity, wall shear stress and pressure gradient are unaffected by the imposed pulsations. In agreement with previous studies of pulsating flow, the phase averaged pressure gradient leads both the velocity and wall shear stress when a single pulsation is imposed on the mean flow. These phase leads remain virtually unchanged when the two frequencies are imposed simultaneously. The amplitude responses of the velocity, wall shear stress and pressure gradient in the combined pulsating flow is shown to be superpositions of the amplitudes from the cases of separate pulsations. There are no signs of non-linear interactions between the harmonics.