Tests have been performed at service- and ultimate load levels of a 55 year-old 121.5 m long prestressed concrete bridge. The purpose was to acquire data for enhanced assessment and calibration of such methods. At service-load several truck overpasses and the dynamic response were particularly studied. Some conclusions were: (a) less stiff load-deflection behavior was obtained with a finite element (FE) analysis compared to measurements and (b) good agreement was obtained for predicted and tested dynamic characteristics. The focus in the destructive tests was on the overall bridge behavior and the ultimate load-carrying capacity of the bridge’s girders and slab. The results were: (a) combined flexure-shear failure of girders occurred after reinforcement yielding for a load indicating an appreciable safety margin in relation to code predictions and (b) good agreement was obtained between FE analysis and the ultimate response and capacity of the tested bridge