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Fine-tuned enzymatic hydrolysis of organosolv pretreated forest materials for the efficient production of cellobiose
Luleå tekniska universitet, Institutionen för samhällsbyggnad och naturresurser, Kemiteknik.ORCID-id: 0000-0001-9164-7667
Luleå tekniska universitet, Institutionen för samhällsbyggnad och naturresurser, Kemiteknik. School of Chemical Engineering, National Technical University of Athens.ORCID-id: 0000-0003-0078-5904
Luleå tekniska universitet, Institutionen för samhällsbyggnad och naturresurser, Kemiteknik.ORCID-id: 0000-0002-3687-6173
Luleå tekniska universitet, Institutionen för samhällsbyggnad och naturresurser, Kemiteknik.ORCID-id: 0000-0001-7500-2367
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2018 (Engelska)Ingår i: Frontiers in Chemistry, E-ISSN 2296-2646, Vol. 6, artikel-id 128Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Non-digestible oligosaccharides (NDOs) are likely prebiotic candidates that have been related to the prevention of intestinal infections and other disorders for both humans and animals. Lignocellulosic biomass is the largest carbon source in the biosphere, therefore cello-oligosacharides (COS), especially cellobiose, are potentially the most widely available choice of NDOs. Production of COS and cellobiose with enzymes offers numerous benefits over acid-catalyzed processes, as it is milder, environmentally friendly and produces fewer by-products. Cellobiohydrolases (CBHs) and a class of endoglucanases (EGs), namely processive EGs, are key enzymes for the production of COS, as they have higher preference toward glycosidic bonds near the end of cellulose chains and are able to release soluble products. In this work, we describe the heterologous expression and characterization of two CBHs from the filamentous fungus Thermothelomyces thermophila, as well as their synergism with proccessive EGs for cellobiose release from organosolv pretreated spruce and birch. The properties, inhibition kinetics and substrate specific activities for each enzyme are described in detail. The results show that a combination of EGs belonging to Glycosyl hydrolase families 5, 6 and 9, with a CBHI and CBHII in appropriate proportions, can enhance the production of COS from forest materials, underpinning the potential of these biocatalysts in the production of NDOs.

Ort, förlag, år, upplaga, sidor
Frontiers Media S.A., 2018. Vol. 6, artikel-id 128
Nyckelord [en]
cellobiohydrolases, hydrolysis, enzymatic cocktail, cellobiose, experimental design, thermostable enzymes, prebiotics
Nationell ämneskategori
Teknik och teknologier Bioprocessteknik
Forskningsämne
Biokemisk processteknik
Identifikatorer
URN: urn:nbn:se:ltu:diva-68257DOI: 10.3389/fchem.2018.00128ISI: 000430398300001PubMedID: 29725590Scopus ID: 2-s2.0-85047449575OAI: oai:DiVA.org:ltu-68257DiVA, id: diva2:1196423
Projekt
"Food-grade prebiotic oligosaccharide production, merging marine and forest resources for moving up the cellulose value-chain (ForceUpValue)"
Forskningsfinansiär
VINNOVA, 158526
Anmärkning

Validerad;2018;Nivå 2;2018-05-03 (andbra)

Tillgänglig från: 2018-04-10 Skapad: 2018-04-10 Senast uppdaterad: 2023-09-05Bibliografiskt granskad

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Karnaouri, Anthi CTopakas, EvangelosMatsakas, LeonidasRova, UlrikaChristakopoulos, Paul

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Karnaouri, Anthi CTopakas, EvangelosMatsakas, LeonidasRova, UlrikaChristakopoulos, Paul
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Frontiers in Chemistry
Teknik och teknologierBioprocessteknik

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