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Comparison between process simulation and deformation measured by defocused speckle photography
Luleå University of Technology, Department of Engineering Sciences and Mathematics, Fluid and Experimental Mechanics.
Luleå University of Technology, Department of Engineering Sciences and Mathematics, Fluid and Experimental Mechanics.ORCID iD: 0000-0001-8355-2414
Luleå University of Technology, Department of Engineering Sciences and Mathematics, Fluid and Experimental Mechanics.ORCID iD: 0000-0002-7566-3656
Luleå University of Technology, Department of Engineering Sciences and Mathematics, Product and Production Development.
Show others and affiliations
2018 (English)In: Speckle 2018: VII International Conference On Speckle Metrology / [ed] M. Kujawińska ; L. R. Jaroszewicz, SPIE - International Society for Optical Engineering, 2018, Vol. 10834, article id 108341KConference paper, Published paper (Refereed)
Abstract [en]

Defocused laser speckle photography is used as a tool to measure the heat responses in a titanium component during laser heating. The evolution of the response is compared with a set of preprocessed Finite Element Simulations of the corresponding process with the aim to verify the simulation model and to find the simulation settings that best resemble the experimental results. The titanium component consists of a 300 x 100 mm2 substrate of thickness 3.2 mm on which a 200 x 30 x 11 mm3 ridge is built up using the laser metal deposition by wire process. The component is heated on the top of the ridge by a 300 W laser for 10 s and the deformation of the subtrate is followed throughout the heating-cooling cycle. The simulated deformation gradient is shown to resemble the measured response, and the magnitude of the response indicates that about 70 % of the laser power transferres into heat in the metal.

Place, publisher, year, edition, pages
SPIE - International Society for Optical Engineering, 2018. Vol. 10834, article id 108341K
Series
Proceedings of SPIE - the International Society for Optical Engineering, ISSN 0277-786X ; 10834
Keywords [en]
Laser speckle, deformation, in-process measurement
National Category
Applied Mechanics Other Mechanical Engineering
Research subject
Experimental Mechanics; Computer Aided Design
Identifiers
URN: urn:nbn:se:ltu:diva-71895DOI: 10.1117/12.2319384ISI: 000455052600052Scopus ID: 2-s2.0-85058383509ISBN: 9781510622975 (print)OAI: oai:DiVA.org:ltu-71895DiVA, id: diva2:1268287
Conference
SPECKLE 2018: VII International Conference on Speckle Metrology, 9-12 September 2018, Janów Podlaski, Poland.
Funder
VINNOVAAvailable from: 2018-12-05 Created: 2018-12-05 Last updated: 2019-12-11Bibliographically approved
In thesis
1. Development of Methods to Investigate Pulmonary Arterial Smooth Muscle Cells under Hypoxia
Open this publication in new window or tab >>Development of Methods to Investigate Pulmonary Arterial Smooth Muscle Cells under Hypoxia
2019 (English)Licentiate thesis, comprehensive summary (Other academic)
Abstract [en]

Hypoxic pulmonary vasoconstriction (HPV) is a physiological response to localized alveolarhypoxia that is intrinsic to the pulmonary circulation. By hypoxia-induced contractionof pulmonary arterial smooth muscle cells (PASMCs), the pulmonary capillary bloodflow is redirected to alveolar areas of high oxygen partial pressure, thus maintaining theventilation-perfusion ratio. Although the principle of HPV was recognized decades agothe underlying pathway remains elusive. The patch clamp technique, imaging and Ramanspectroscopy are methods that can be used to investigate parts of the mechanisms. Toenable measurements at controlled oxygen concentrations a gas-tight microfluidic systemwas developed. In this thesis preparatory experiments to couple the gas-tight systemto a microscope that enabled simultaneous measurements with patch clamp, imagingand Raman spectroscopy are discussed. The patch clamp technique is to be used formeasurements on the dynamics of the ion-channels in the cellular membrane as well aschanges in membrane potential as a response to hypoxia. Imaging of PASMCs is requiredto successfully apply the patch clamp technique. Further, imaging will also reveal whetherthe mechanical response of HPV has been triggered, for this purpose image analysis forestimation of optical flow can be used. Raman spectroscopy enables measurements ofbiochemical changes in redox biomarkers, cytochrome c and NADH, of the mitochondrialelectron transport chain. This thesis shows that the gas-tight microfluidic system providesoptimal control of the oxygen content, in an experimantal setting where the patch clamptechnique can be applied. Raman measurements showed significantly larger variationsin spectra compared to an open fluidic system, which is the conventional approach.However, the results showed a need for improved Raman preprocessing. For this purposea Convolutional Neural Network (CNN) was trained using synthetic spectra that providedoptimal reconstruction of the Raman signal. Finally, simultaneous imaging and Ramanspectroscopy of red blood cells were performed in a home built microscope. The resultspave the way for measurements on PASMCs.

Place, publisher, year, edition, pages
Luleå: Luleå University of Technology, 2019
Series
Licentiate thesis / Luleå University of Technology, ISSN 1402-1757
National Category
Medical Engineering Applied Mechanics
Research subject
Experimental Mechanics
Identifiers
urn:nbn:se:ltu:diva-77140 (URN)978-91-7790-513-4 (ISBN)978-91-7790-514-1 (ISBN)
Presentation
2020-01-24, E237, Luleå, 10:00 (English)
Opponent
Supervisors
Available from: 2019-12-11 Created: 2019-12-11 Last updated: 2020-03-12Bibliographically approved

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Wahl, JoelGren, PerBergström, PerLindbäck, TorbjörnSjödahl, Mikael

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