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Optimization of the production of docosahexaenoic fatty acid by the heterotrophic microalga Crypthecodinium cohnii utilizing a dark fermentation effluent
Industrial Biotechnology & Biocatalysis Group, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Zografou Campus, Athens, Greece.
Industrial Biotechnology & Biocatalysis Group, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Zografou Campus, Athens, Greece.
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering. Industrial Biotechnology & Biocatalysis Group, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Zografou Campus, Athens, Greece.ORCID iD: 0000-0003-0078-5904
2020 (English)In: Renewable energy, ISSN 0960-1481, E-ISSN 1879-0682, Vol. 152, p. 102-109Article in journal (Refereed) Published
Abstract [en]

Dark fermentation is an anaerobic digestion process of biowaste, used to produce hydrogen as a fuel, which however releases high amounts of polluting volatile fatty acids in the environment. In order for the process to become more competitive, the acids stream can be utilized through conversion to high added-value docosahexaenoic acid by the microalga Crypthecodinium cohnii. Docosahexaenoic acid is one of the two main omega-3 fatty acids, necessary for human nutrition. The purpose of this work was to optimize the production of omega-3 fatty acids by the cells, utilizing the organic content of a dark fermentation effluent. For that purpose, the effect of different fermentation conditions was examined, such as incubation temperature, nitrogen source and concentration, the addition of chemical modulators, as well as the feeding composition. The volatile fatty acid content of the effluent was totally depleted in a fed-batch culture of the microalga, while the cells accumulated DHA in a percentage of 35.6% of total lipids, when fed with yeast extract or 34.2% when fed with ammonium sulfate. Taking into consideration the economic feasibility of the culture conditions proposed it was concluded that the use of yeast extract could be substituted by the much economic ammonium sulfate.

Place, publisher, year, edition, pages
Elsevier, 2020. Vol. 152, p. 102-109
Keywords [en]
Crypthecodinium cohnii, Biorefinery, Volatile fatty acids, Dark fermentation effluent, Docosahexaenoic acid
National Category
Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
URN: urn:nbn:se:ltu:diva-78552DOI: 10.1016/j.renene.2020.01.041ISI: 000536949600010Scopus ID: 2-s2.0-85078006722OAI: oai:DiVA.org:ltu-78552DiVA, id: diva2:1424071
Note

Validerad;2020;Nivå 2;2020-04-16 (johcin)

Available from: 2020-04-16 Created: 2020-04-16 Last updated: 2020-07-01Bibliographically approved

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Topakas, Evangelos

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