Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Metabolt: An In-Situ Instrument to Characterize the Metabolic Activity of Microbial Soil Ecosystems Using Electrochemical and Gaseous Signatures
Luleå University of Technology, Department of Computer Science, Electrical and Space Engineering, Space Technology.ORCID iD: 0000-0002-7148-8803
Luleå University of Technology, Department of Computer Science, Electrical and Space Engineering, Space Technology. Centro de Astrobiología (CSIC-INTA), Torrejon de Ardoz, 28850 Madrid, Spain. School of Geosciences, University of Aberdeen, Meston Building, King’s College, Aberdeen AB24 3UE, UK.ORCID iD: 0000-0002-4492-9650
Luleå University of Technology, Department of Computer Science, Electrical and Space Engineering, Space Technology. School of Geosciences, University of Aberdeen, Meston Building, King’s College, Aberdeen AB24 3UE, UK. Instituto Andaluz de Ciencias de la Tierra (CSIC-UGR), 18100 Granada, Spain.ORCID iD: 0000-0001-6479-2236
2020 (English)In: Sensors, E-ISSN 1424-8220, Vol. 20, no 16, article id 4479Article in journal (Refereed) Published
Abstract [en]

Metabolt is a portable soil incubator to characterize the metabolic activity of microbial ecosystems in soils. It measures the electrical conductivity, the redox potential, and the concentration of certain metabolism-related gases in the headspace just above a given sample of regolith. In its current design, the overall weight of Metabolt, including the soils (250 g), is 1.9 kg with a maximum power consumption of 1.5 W. Metabolt has been designed to monitor the activity of the soil microbiome for Earth and space applications. In particular, it can be used to monitor the health of soils, the atmospheric-regolith fixation, and release of gaseous species such as N2, H2O, CO2, O2, N2O, NH3, etc., that affect the Earth climate and atmospheric chemistry. It may be used to detect and monitor life signatures in soils, treated or untreated, as well as in controlled environments like greenhouse facilities in space, laboratory research environments like anaerobic chambers, or simulating facilities with different atmospheres and pressures. To illustrate its operation, we tested the instrument with sub-arctic soil samples at Earth environmental conditions under three different conditions: (i) no treatment (unperturbed); (ii) sterilized soil: after heating at 125 °C for 35.4 h (thermal stress); (iii) stressed soil: after adding 25% CaCl2 brine (osmotic stress); with and without addition of 0.5% glucose solution (for control). All the samples showed some distinguishable metabolic response, however there was a time delay on its appearance which depends on the treatment applied to the samples: 80 h for thermal stress without glucose, 59 h with glucose; 36 h for osmotic stress with glucose and no significant reactivation in the pure water case. This instrument shows that, over time, there is a clear observable footprint of the electrochemical signatures in the redox profile which is complementary to the gaseous footprint of the metabolic activity through respiration.

Place, publisher, year, edition, pages
MDPI, 2020. Vol. 20, no 16, article id 4479
Keywords [en]
Metabolt, space, electrical conductivity, redox potential, gas monitoring, microbial metabolism, astrobiology, greenhouses, planetary analogue research, planetary exploration
National Category
Aerospace Engineering
Research subject
Atmospheric science
Identifiers
URN: urn:nbn:se:ltu:diva-80556DOI: 10.3390/s20164479ISI: 000568172200001PubMedID: 32796545Scopus ID: 2-s2.0-85089390322OAI: oai:DiVA.org:ltu-80556DiVA, id: diva2:1460950
Note

Validerad;2020;Nivå 2;2020-08-25 (alebob)

Available from: 2020-08-25 Created: 2020-08-25 Last updated: 2022-02-10Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records

Israel Nazarious, MiracleZorzano, María-PazMartín-Torres, Javier

Search in DiVA

By author/editor
Israel Nazarious, MiracleZorzano, María-PazMartín-Torres, Javier
By organisation
Space Technology
In the same journal
Sensors
Aerospace Engineering

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 41 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf