The Mauritian Flying Fox (Pteropus niger), familiar as Greater Mascarene Flying Fox (Mauritius Fruit Bat), a large bat species common to Mauritius and La Ré-union. Mauritian Flying Fox is vulnerable to extinction due to lack of concern in their conservation, particularly when they are perceived as a threat to urban life. Preservative initiatives among the public are subject to awareness and perception. Assessment of public attitude and perception in the case of elusive animals is quite challenging. The paper examines public awareness, experience and threat encountered, perception, willing to preserve Mauritian Flying Fox, along with significant socio-demographic factors. NEP scale is used to test the willingness to accept these mammals. Using a five-point Likert scale questionnaire, a drop-off survey is conducted. The response rate is satisfactory (KMO and Bartlet: significant at 0.5, 0.7, 0.7, and 0.8) and suitable for further analysis. Factor Analysis (FA), Confirmatory Factor Analysis (CFA), Structural Equation Modelling (SEM) is computed followed by a path diagram. Contingency Valuation Method (CVM) is used to test the respondent’s willingness (Willingness to Accept) in preserving these mammals. Respondents are having decent information about the ecosystem and perceived these mammals positively, while few respondents have an adverse approach towards these mammals and support culling. This may be due to ignorance and lack of awareness in the ecological system. The study indicates that the conservation success of Mauritian Flying Fox mainly depends on good awareness programs among the community. Positive perception and attitude mend the willingness to preserve these mammals. The findings of this study recommend more holistic approaches to wildlife conservation (Mauritian Flying Fox) that integrate simultaneously knowledge, attitude, perception, and willingness to preserve wildlife perfection.