CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches
Translational Nanobiology Section, Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA; Translational Nanobiology Section, Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Nuffield Department of Women’s and Reproductive Health, University of Oxford, Women’s Centre, John Radcliffe Hospital, Oxford, UK.ORCID iD: 0000-0003-0645-6714
School of Pharmacy and Pharmaceutical Sciences, Trinity College Dublin, Dublin, Ireland; Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland; Trinity St. James’s Cancer Institute, Trinity College Dublin, Dublin, Ireland.
Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary; HCEMM-SU Extracellular Vesicle Research Group, Semmelweis University, Budapest, Hungary; HUN-REN-SU Translational Extracellular Vesicle Research Group, Semmelweis University, Budapest, Hungary.
Show others and affiliations
2024 (English)In: Journal of Extracellular Vesicles, E-ISSN 2001-3078, Vol. 13, no 2, article id e12404Article in journal (Refereed) Published
Abstract [en]

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

Place, publisher, year, edition, pages
John Wiley and Sons Inc , 2024. Vol. 13, no 2, article id e12404
Keywords [en]
ectosomes, exosomes, extracellular particles, extracellular vesicles, guidelines, microparticles, microvesicles, minimal information requirements, MISEV, reproducibility, rigor, standardisation
National Category
Cell Biology
Research subject
Biomedical Engineering
Identifiers
URN: urn:nbn:se:ltu:diva-104901DOI: 10.1002/jev2.12404PubMedID: 38326288Scopus ID: 2-s2.0-85183359756OAI: oai:DiVA.org:ltu-104901DiVA, id: diva2:1847022
Note

Godkänd;2024;Nivå 0;2024-04-05 (marisr);

Full text license: CC BY

Available from: 2024-03-26 Created: 2024-03-26 Last updated: 2024-04-05Bibliographically approved

Open Access in DiVA

fulltext(2522 kB)48 downloads
File information
File name FULLTEXT01.pdfFile size 2522 kBChecksum SHA-512
bb02abd58709951ddb473788fc65002f443dce613061330b58103a740b746fcfce7aaebb645718422b3c105cccc487e1180ddce7ee86db2cfab9b0d485ee26e9
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMedScopus

Authority records

Kumar, Saroj

Search in DiVA

By author/editor
Goberdhan, Deborah C. I.Bussolati, BenedettaHill, Andrew F.Mohanty, SujataOchiya, TakahiroTorrecilhas, Ana C.Zheng, LeiMartens-Uzunova, Elena S.Newman, Lauren A.Wauben, Marca H. M.Wehman, Ann M.Kumar, Saroj
By organisation
Nursing and Medical Technology
In the same journal
Journal of Extracellular Vesicles
Cell Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 52 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 209 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf