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Methylmercury measurement in whole blood by isotope-dilution GC-ICPMS with 2 sample preparation methods
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Geosciences and Environmental Engineering.ORCID iD: 0000-0003-4505-4590
ALS Analytica AB, Luleå.
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2007 (English)In: Clinical Chemistry, ISSN 0009-9147, E-ISSN 1530-8561, Vol. 53, no 1, p. 111-116Article in journal (Refereed) Published
Abstract [en]

Background: Despite its known toxicity, methylmercury is rarely measured directly in clinical studies; instead, conclusions are based on total mercury measurements. We have developed isotope-dilution-based methods for methylmercury-specific analysis of whole blood by coupled gas chromatography-inductively coupled plasma mass spectrometry (GC-ICPMS). Methods: We analyzed animal and human blood samples after alkaline digestion or extraction of methylmercury into dichloromethane and back extraction into water. Methylmercury was converted to the volatile ethyl derivative, purged, and trapped on a solid-phase collection medium, and then introduced into the GC-ICPMS system. Results: Limits of quantification were 0.4 and 0.03 mu g/L at a signal-to-noise ratio of 10 with the alkaline digestion and extraction methods, respectively. Extraction met our selected acceptable total error criterion, with an SD of 0.58 mu g/L at the critical maternal blood concentration of 5.8 mu g/L.Results obtained with alkaline digestion indicated the need for improved random analytical uncertainty, which was achieved by increasing the enrichment of the isotope dilution. For 37 blood samples, the mean (SD) proportion of total mercury present as methylmercury was 60 (27)%, range 6%-100%.Conclusions: The combination of extraction and isotope-dilution GC-ICPMS meets the requirements for use as a reference method for measuring methylmercury in whole blood.

Place, publisher, year, edition, pages
2007. Vol. 53, no 1, p. 111-116
National Category
Geochemistry
Research subject
Applied Geology
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URN: urn:nbn:se:ltu:diva-4432DOI: 10.1373/clinchem.2007.072520ISI: 000243233200017Scopus ID: 2-s2.0-33846023734Local ID: 260607c0-677b-11dc-a0c3-000ea68e967bOAI: oai:DiVA.org:ltu-4432DiVA, id: diva2:977305
Note
Validerad; 2007; 20070920 (pirkko)Available from: 2016-09-29 Created: 2016-09-29 Last updated: 2018-07-10Bibliographically approved

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Baxter, DouglasRodushkin, IlyaEngström, Emma

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