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The use of cellulases and hemicellulases to study lignin-cellulose as well as lignin-hemicellulose bonds in kraft pulps
Luleå University of Technology, Department of Engineering Sciences and Mathematics, Wood Science and Engineering.ORCID iD: 0000-0002-7711-9267
2001 (English)In: Journal of Pulp and Paper Science (JPPS), ISSN 0826-6220, Vol. 27, no 6, p. 196-201Article in journal (Refereed) Published
Abstract [en]

The combination of carbohydrate-degrading enzymes and size-exclusion chromatography (SEC) has been used to analyze the existence of covalent bonds between lignin and cellulose and/or hemicelluloses in pulps. Cellulases and xylanase were used for degradation of cellulose and xylan, respectively. Analysis of the molecular weight profiles of lignin and carbohydrates before and after enzymic hydrolysis were performed by dissolution of the pulps in LiCl/dimethylacetamide (DMAC). The results indicate that a considerable part of the residual lignin in unbleached pine kraft pulp is bond to cellulose. Bonding of lignin to xylan in the pine kraft pulp could also be detected. Analysis of birch kraft pulp shows that most of the residu lignin in birch kraft pulps is covalently linked to xylan. The combination of carbohydrate-degrading enzymes and SEC in LiCl/DMAC seems to be an excellent way of characterizing bonds between lignin and carbohydrates in chemical pulps.

Place, publisher, year, edition, pages
2001. Vol. 27, no 6, p. 196-201
National Category
Bio Materials
Research subject
Wood Physics
Identifiers
URN: urn:nbn:se:ltu:diva-13950Local ID: d44d2170-a3f2-11dc-8fee-000ea68e967bOAI: oai:DiVA.org:ltu-13950DiVA, id: diva2:986903
Note
Validerad; 2001; 20071206 (cira)Available from: 2016-09-29 Created: 2016-09-29 Last updated: 2017-11-24Bibliographically approved

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Karlsson, OlovWestermark, Ulla

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