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Development of a novel Raman tweezers setup for single-cell studies
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2003 (English)In: Manipulation and Analysis of Biomolecules, Cells, and Tissues, Bellingham, Wash: SPIE - International Society for Optical Engineering, 2003, p. 271-277Conference paper, Published paper (Refereed)
Abstract [en]

It has recently been shown that the combination of Raman spectroscopy and optical tweezers constitute a powerful tool for biological studies. Raman spectra of single cells immobilized in a sterile surrounding can then be recorded without the risk of surface-induced morphological cell changes. Further, the complete cellular environment can be changed while measuring dynamics in real time. We here introduce a novel Raman tweezers set-up ideal for resonance Raman studies of single cells. The system differs from earlier set-ups in that two separate laser beams, used for trapping and Raman excitation, are combined in a double-microscope configuration. This has the advantage that the wavelength and power of the trapping and probe beam can be adjusted individually, for example in order to optimize the functionality of the set-up or to record resonance Raman profiles from the same trapped cell. Further, the tweezers can be removed from the system without affecting the spectrometer configuration. Trapping is achieved by tightly focusing IR diode laser radiation (830 nm) through an inverted oil immersion objective with high numerical aperture (NA = 1.25), while Raman scattering is excited by the lines of an ArKr ion-laser. The backscattered Raman signal is collected by a single-grating spectrometer equipped with a microscope and a 60x water-immersion objective (NA = 0.9). The functionality of the system is demonstrated by measurements of trapped single functional erythrocytes using differen excitation lines (488, 514.5 568.2 nm) in resonance with the heme moiety and by studying the spectral evolution during illumination.

Place, publisher, year, edition, pages
Bellingham, Wash: SPIE - International Society for Optical Engineering, 2003. p. 271-277
Series
Proceedings of the Society of Photo-Optical Instrumentation Engineers ; 4962
National Category
Other Medical Engineering
Research subject
Medical Engineering for Healthcare
Identifiers
URN: urn:nbn:se:ltu:diva-28495DOI: 10.1117/12.502199Local ID: 24f74d40-cbf3-11db-b3ed-000ea68e967bISBN: 9780819447623 (print)OAI: oai:DiVA.org:ltu-28495DiVA, id: diva2:1001693
Conference
Manipulation and Analysis of Biomolecules, Cells and Tissues : 28/01/2003 - 29/01/2003
Note
Upprättat; 2003; 20070306 (ysko)Available from: 2016-09-30 Created: 2016-09-30 Last updated: 2017-11-25Bibliographically approved

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Ramser, Kerstin

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