Sulfide mineral flotation is known to be affected by oxidation-reduction conditions in the pulp, although there is some doubt as to whether it is the oxidizing environment or the type of oxidant which influences flotation. Variations in the oxidizing conditions of the pulp and their subsequent effect on flotation were examined for two types of natural sulphide ore, a galena-sphalerite ore and a complex pyrite ore. Different gas mixtures ranging from pure nitrogen to pure oxygen were used to control the oxidizing conditions. Zinc recovery increased with a more oxidizing environment, and nitrogen gas acted almost as a depressant for sphalerite in the flotation of the Pb-Zn ore. For the complex pyrite ore, the selectivity between chalcopyrite and both gangue and pyrite improved when the environment was oxidizing, resulting in higher Cu recovery in the copper rougher concentrate.