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Use of pyranine as a trace fluorescent probe to study structure in aqueous sucrose solutions
Michigan State University.
1991 (English)In: Particle design via crystallization / [ed] Ramu Ramanarayanan, Washington, DC: American Institute of Chemical Engineers, 1991, p. 114-123Conference paper, Published paper (Refereed)
Abstract [en]

Emission spectroscopy using pyranine as a trace fluorescent probe was evaluated as a means of studying solution structure in aqueous sucrose solutions. Spectra of pyranine doped sucrose solutions of 0-70 wt% were obtained, indicating two microenvironments. The peak intensity ratio (defined as the ratio of peak intensities at 440 nm and 511 nm) was observed to be a very sensitive function of the water content in supersaturated solutions. The two microenvironments were designated as bulk water and that near the solvated sucrose molecules. Calculations yielded six water molecules of solvation per molecule of sucrose. This is in agreement with values in literature. The number of molecules of water per molecule of sucrose in the bulk water environment varies exponentially from 165 in a 10% solution to three for a 70% solution at 20 degrees C. These results indicate that this technique can be used successfully to measure water content in supersaturated solutions.

Place, publisher, year, edition, pages
Washington, DC: American Institute of Chemical Engineers, 1991. p. 114-123
Series
AIChE Symposium Series, ISSN 0065-8812 ; 284
National Category
Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
URN: urn:nbn:se:ltu:diva-31345Local ID: 580b2730-3ecc-11de-bf5b-000ea68e967bISBN: 0-8169-0553-3 (print)OAI: oai:DiVA.org:ltu-31345DiVA, id: diva2:1004579
Conference
American Institute of Chemical Engineers. National Meeting : 17/12/1991
Note
Upprättat; 1991; 20090512 (ysko)Available from: 2016-09-30 Created: 2016-09-30 Last updated: 2017-11-25Bibliographically approved

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