Cellulose particles in micro and nano scales has shown excellent potential to adsorb water pollutants such as dyes, pesticides, bacteria and virus, and a wide range of heavy metal ions, including Ag(Ⅰ), U(Ⅱ), Fe(Ⅲ), Cu(Ⅱ), Ni(Ⅱ), Cr(Ⅲ) and Zn(Ⅱ) 1, 2. However, mechanisms of adsorption and desorption the contaminants to/from cellulose micro or nano particles are largely unknown. The aim of the study was to develop fundamental understanding about the interaction and adsorption behavior of silver ions on cellulose surfaces using colloidal probe and spectroscopy techniques. Force interactions between three types of cellulose microspheres viz. native cellulose microspheres (CM), sulfate cellulose microspheres (SCM), phosphate cellulose microspheres (PCM) and silica surface in AgNO3 solution were studied with atomic force microscopy (AFM). The AFM results were further elaborated by extensive spectroscopy investigations.