One of the most common causes of failures in total joint replacements is the generation of wear particles within the joint that leads to the micro separation at the implant-bone interface. This contributes to bone lost aseptic loosening of the implant, requiring eventually its replacement.Many studies have been carried out to improve the wear characteristics of bearing surfaces in total joint replacement (TJR). From lubrication point of view the friction behavior of surfaces and rheology of the joint lubricant (Synovial fluid) were extensively studied. However, not much attention was paid to the interaction between the lubricant and the bearing surfaces. The aim of this study is to develop a methodology for studying the behavior of water based lubricant in a micro-channel. For this purpose, Micro-PIV (Particle Image velocimetry) was used in order to characterize the lubricant behavior. Experimental models made of relevant materials such as ultra high molecular weight polyethylene, Cobalt-Chromium-Molybdenum alloy and Titanium-Aluminum-Vanadium alloy with 1 and 1.5 mm width, 45 mm length and 2 mm depth experimentally investigated.