A cross sectional epidemiological study was carried out to explore the relationship between perception of musculoskeletal symptoms and cold exposure. The study was applied in a large meat processing company in Colombia. The standardized Nordic Questionnaire was applied to 162 workers working in this company: 50 workers from cold areas (+2°C) and 112 workers from non-exposed areas (+9.4°C). Several environmental measurements were applied in both areas: air velocity, web bulb and dry temperature and humidity. The results of the research showed high prevalence of perception of musculoskeletal symptoms among cold-exposed workers, especially for low back, neck and shoulders (48%, 36% and 24%). The estimated relative risk for neck and low back were 11.2 (95% CI 1.34 – 93.41) and 4.48 (95% CI 1.61 – 12.42) respectively. Other association to cold exposition such as discomfort with cold draughts and thermal protective clothing were not evident in this research. The work shift, the type of contract with company and psychosocial work-related risks were not controlled in this research, which could have had some influence upon the results. The association between cold exposure and musculoskeletal problems are mainly still obscure and the need for further research is necessary from experimental designs and epidemiology studies (case-control or preferably cohort studies) that look to analyse the causes.