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Protein-coated nanoparticles embedded in films as delivery platforms
Luleå University of Technology, Department of Health Sciences, Medical Science. College of Pharmacy, University of Texas at Austin.
Ferring Controlled Therapeutics Ltd.
University of New Mexico.
2013 (English)In: Journal of Pharmacy and Pharmacology (JPP), ISSN 0022-3573, E-ISSN 2042-7158, Vol. 65, no 6, 827-838 p.Article in journal (Refereed) Published
Abstract [en]

OBJECTIVES: This work aimed to evaluate the performance of nanoparticle-loaded films based on matrices of polymethacrylates and hydroxypropylmethylcellulose (HPMC) intended for delivery of macromolecules.

METHODS: Lysozyme (Lys)-loaded nanoparticles were manufactured by antisolvent co-precipitation. After size, loading efficiency and stability characterization, the selected batch of particles was further formulated into films. Films were characterized for mechanical properties, mucoadhesion, Lys release and activity after manufacture.

KEY FINDINGS: We found that protein-coated nanoparticles could be obtained in USP phosphate buffer pH 6.8. Particles obtained at pH 6.8 had a z-average of 347.2 nm, a zeta-potential of 21.9 mV and 99.2% remaining activity after manufacture. This formulation was further studied for its application in films for buccal delivery. Films loaded with nanoparticles that contained Eudragit RLPO (ERL) exhibited excellent mechanical and mucoadhesive properties. Due to its higher water-swelling and solubility compared with ERL, the use of HPMC allowed us to tailor the release of Lys from films. The formulation composed of equal amounts of ERL and HPMC revealed a sustained release over 4 h, with Lys remaining fully active at the end of the study.

CONCLUSIONS: Mucoadhesive films containing protein-coated nanoparticles are promising carriers for the buccal delivery of proteins and peptides in a stable form.

Place, publisher, year, edition, pages
2013. Vol. 65, no 6, 827-838 p.
National Category
Pharmaceutical Sciences Other Health Sciences
Research subject
Health Science
Identifiers
URN: urn:nbn:se:ltu:diva-64673DOI: 10.1111/jphp.12046PubMedID: 23647676Scopus ID: 2-s2.0-84877575080OAI: oai:DiVA.org:ltu-64673DiVA: diva2:1119610
Available from: 2017-07-04 Created: 2017-07-04 Last updated: 2017-07-05Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
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