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Effect of synthetic and natural media on lipid production from Fusarium oxysporum
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.ORCID iD: 0000-0002-3687-6173
Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens.
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.
2017 (English)In: Electronic Journal of Biotechnology, ISSN 0717-3458, E-ISSN 0717-3458, Vol. 30, 95-102 p.Article in journal (Refereed) Published
Abstract [en]

Background

Dependence on fossil resources, for the production of fuels and energy, has resulted in environmental and financial problems, which require our immediate action in order to reverse the situation. Use of renewable sources for the production of fuels and energy is an important alternative with biodiesel remains as one of the promising options. Aim of this work is to evaluate the fungus Fusarium oxysporum for its potentials to accumulate microbial lipids when grown on synthetic media and saccharified sweet sorghum stalks.

Results

The effect of different carbon sources, nitrogen sources and C/N ratio on the lipid production was initially examined, which resulted in a lipid concentration of 4.4 g/L, with lipid content of 42.6% w/w. Sweet sorghum stalks were able to support growth and lipid production of the fungus, both as carbon source and as nitrogen source. It was also shown that saccharification of the dried stalks is an important step to increase lipid production. Removal of the remaining stalk solids enabled the lipid production during cultivation in increased initial solids of up to 16 w/w. This resulted in a lipid production of 3.81 g/L.

Conclusions

It was demonstrated that F. oxysporum can be used as an efficient oleaginous microorganism, with sweet sorghum serving as an excellent raw material for the cultivation of the fungus. The lipids obtained during this work were also found to have a fatty acid profile with good potentials to be used for biodiesel production.

Place, publisher, year, edition, pages
Elsevier, 2017. Vol. 30, 95-102 p.
National Category
Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
URN: urn:nbn:se:ltu:diva-66028DOI: 10.1016/j.ejbt.2017.10.003ISI: 000416721500016Scopus ID: 2-s2.0-85034096464OAI: oai:DiVA.org:ltu-66028DiVA: diva2:1148244
Note

Validerad;2017;Nivå 2;2017-12-05 (rokbeg)

Available from: 2017-10-10 Created: 2017-10-10 Last updated: 2017-12-14Bibliographically approved

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