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Realization of Peptone Biosensor Based on Newly Prepared NiO Nanostructures
Institute of Biotechnology and Genetic Engineering, University of Sindh-76080, Jamshoro, Pakistan.ORCID iD: 0000-0001-6777-5642
Institute of Biotechnology and Genetic Engineering, University of Sindh-76080, Jamshoro, Pakistan.
Dr. M. A. Kazi Institute of Chemistry University of Sindh-76080, Jamshoro, Pakistan.
Institute of Biotechnology and Genetic Engineering, University of Sindh-76080, Jamshoro, Pakistan.
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2017 (English)In: Sensor Letters, ISSN 1546-198X, E-ISSN 1546-1971, Vol. 15, no 10, p. 822-826Article in journal (Refereed) Published
Abstract [en]

The present study authenticates the fabrication of nickel oxide porous shaped nanostructure by hydrothermal method. The novel and functionalized nickel oxide nanomaterial were visualized by using scanning electron microscopy (SEM) and X-ray diffraction techniques (XRD). NiO nanomaterial advertised sensitive, selective and attracted morphology for the development of peptone biosensor. Phenylalanine displays a soft template and growth directing agent for the developing of nickel oxide low dimension nanostructures. The nickel oxide nanomaterial together with protease possesses tremendous role towards the oxidation potential phenomena and transfer of anodic electro-catalytic current for the peptone. The generation of low potential electrochemical signals exhibited the determination of peptone by utilizing different electrochemical techniques for the given concentration ranging from 0.1 mM to 2.5 mM with the measured limit of detection about 0.002 mM with a sensitivity of 107200 μA/mMCm2. The well-defined and highly developed sensor system provides the standard platform for the fabrication and functioning of new devices that are helpful for the determination of many biological macromolecules. The presented peptone biosensor is highly selective, sensitive, and reproducible that could also be useful for the determination of peptone from various milk samples.

Place, publisher, year, edition, pages
American Scientific Publishers, 2017. Vol. 15, no 10, p. 822-826
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Materials Chemistry Other Physics Topics
Research subject
Experimental physics
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URN: urn:nbn:se:ltu:diva-67598DOI: 10.1166/sl.2017.3874OAI: oai:DiVA.org:ltu-67598DiVA, id: diva2:1181884
Note

Validerad;2018;Nivå 1;2018-02-12 (rokbeg)

Available from: 2018-02-10 Created: 2018-02-10 Last updated: 2018-04-26Bibliographically approved

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