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Fine-tuned enzymatic hydrolysis of organosolv pretreated forest materials for the efficient production of cellobiose
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering. School of Chemical Engineering, National Technical University of Athens.ORCID iD: 0000-0003-0078-5904
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.ORCID iD: 0000-0002-3687-6173
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.ORCID iD: 0000-0001-7500-2367
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2018 (English)In: Frontiers in Chemistry, E-ISSN 2296-2646, Vol. 6, article id 128Article in journal (Refereed) Published
Abstract [en]

Non-digestible oligosaccharides (NDOs) are likely prebiotic candidates that have been related to the prevention of intestinal infections and other disorders for both humans and animals. Lignocellulosic biomass is the largest carbon source in the biosphere, therefore cello-oligosacharides (COS), especially cellobiose, are potentially the most widely available choice of NDOs. Production of COS and cellobiose with enzymes offers numerous benefits over acid-catalyzed processes, as it is milder, environmentally friendly and produces fewer by-products. Cellobiohydrolases (CBHs) and a class of endoglucanases (EGs), namely processive EGs, are key enzymes for the production of COS, as they have higher preference toward glycosidic bonds near the end of cellulose chains and are able to release soluble products. In this work, we describe the heterologous expression and characterization of two CBHs from the filamentous fungus Thermothelomyces thermophila, as well as their synergism with proccessive EGs for cellobiose release from organosolv pretreated spruce and birch. The properties, inhibition kinetics and substrate specific activities for each enzyme are described in detail. The results show that a combination of EGs belonging to Glycosyl hydrolase families 5, 6 and 9, with a CBHI and CBHII in appropriate proportions, can enhance the production of COS from forest materials, underpinning the potential of these biocatalysts in the production of NDOs.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2018. Vol. 6, article id 128
Keywords [en]
cellobiohydrolases, hydrolysis, enzymatic cocktail, cellobiose, experimental design, thermostable enzymes, prebiotics
National Category
Engineering and Technology Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
URN: urn:nbn:se:ltu:diva-68257DOI: 10.3389/fchem.2018.00128ISI: 000430398300001Scopus ID: 2-s2.0-85047449575OAI: oai:DiVA.org:ltu-68257DiVA, id: diva2:1196423
Projects
"Food-grade prebiotic oligosaccharide production, merging marine and forest resources for moving up the cellulose value-chain (ForceUpValue)"
Funder
VINNOVA, 158526
Note

Validerad;2018;Nivå 2;2018-05-03 (andbra)

Available from: 2018-04-10 Created: 2018-04-10 Last updated: 2018-06-07Bibliographically approved

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Publisher's full textScopushttps://www.frontiersin.org/articles/10.3389/fchem.2018.00128/abstract

Authority records BETA

Karnaouri, Anthi CTopakas, EvangelosMatsakas, LeonidasRova, UlrikaChristakopoulos, Paul

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