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A comparative study of the enzymatic hydrolysis of batch organosolv-pretreated birch and spruce biomass
Industrial Biotechnology Division, Department of Biology and Biological Engineering, Chalmers University of Technology.
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.ORCID iD: 0000-0002-9868-9031
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.ORCID iD: 0000-0002-3687-6173
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.ORCID iD: 0000-0001-7500-2367
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2018 (English)In: AMB Express, ISSN 2191-0855, E-ISSN 2191-0855, Vol. 8, no 1, article id 114Article in journal (Refereed) Published
Abstract [en]

A shift towards a sustainable and green society is vital to reduce the negative effects of climate change associated with increased CO2 emissions. Lignocellulosic biomass is both renewable and abundant, but is recalcitrant to deconstruction. Among the methods of pretreatment available, organosolv (OS) delignifies cellulose efficiently, significantly improving its digestibility by enzymes. We have assessed the hydrolysability of the cellulose-rich solid fractions from OS-pretreated spruce and birch at 2% w/v loading (dry matter). Almost complete saccharification of birch was possible with 80 mg enzyme preparation/gsolids (12 FPU/gsolids), while the saccharification yield for spruce was only 70%, even when applying 60 FPU/gsolids. As the cellulose content is enriched by OS, the yield of glucose was higher than in their steam-exploded counterparts. The hydrolysate was a transparent liquid due to the absence of phenolics and was also free from inhibitors. OS pretreatment holds potential for use in a large-scale, closed-loop biorefinery producing fuels from the cellulose fraction and platform chemicals from the hemicellulose and lignin fractions respectively.

Place, publisher, year, edition, pages
2018. Vol. 8, no 1, article id 114
National Category
Bioprocess Technology
Research subject
Biochemical Process Engineering
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URN: urn:nbn:se:ltu:diva-70136DOI: 10.1186/s13568-018-0643-yISI: 000438255300003PubMedID: 29992363Scopus ID: 2-s2.0-85049690419OAI: oai:DiVA.org:ltu-70136DiVA, id: diva2:1233773
Note

Validerad;2018;Nivå 2;2018-07-19 (inah)

Available from: 2018-07-19 Created: 2018-07-19 Last updated: 2018-08-09Bibliographically approved

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Nitsos, ChristosMatsakas, LeonidasRova, UlrikaChristakopoulos, Paul

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