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Side-by-side biochemical comparison of two lytic polysaccharide monooxygenases from the white-rot fungus Heterobasidion irregulare on their activity against crystalline cellulose and glucomannan
Department of Molecular Sciences, Swedish University of Agricultural Sciences, Uppsala.
Department of Molecular Sciences, Swedish University of Agricultural Sciences, Uppsala.
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.ORCID iD: 0000-0003-1336-2396
Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences, Uppsala.
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2018 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 13, no 9, article id e0203430Article in journal (Refereed) Published
Abstract [en]

Our comparative studies reveal that the two lytic polysaccharide monooxygenases HiLP-MO9B and HiLPMO9I from the white-rot conifer pathogen Heterobasidion irregulare display clear difference with respect to their activity against crystalline cellulose and glucomannan. HiLPMO9I produced very little soluble sugar on bacterial microcrystalline cellulose (BMCC). In contrast, HiLPMO9B was much more active against BMCC and even released more soluble sugar than the H. irregulare cellobiohydrolase I, HiCel7A. Furthermore, HiLPMO9B was shown to cooperate with and stimulate the activity of HiCel7A, both when the BMCC was first pretreated with HiLPMO9B, as well as when HiLPMO9B and HiCel7A were added together. No such stimulation was shown by HiLPMO9I. On the other hand, HiLPMO9I was shown to degrade glucomannan, using a C4-oxidizing mechanism, whereas no oxidative cleavage activity of glucomannan was detected for HiLPMO9B. Structural modeling and comparison with other glucomannan-active LPMOs suggest that conserved sugar-interacting residues on the L2, L3 and LC loops may be essential for glucomannan binding, where 4 out of 7 residues are shared by HiLPMO9I, but only one is found in HiLPMO9B. The difference shown between these two H. irregulare LPMOs may reflect distinct biological roles of these enzymes within deconstruction of different plant cell wall polysaccharides during fungal colonization of softwood.

Place, publisher, year, edition, pages
Public Library of Science , 2018. Vol. 13, no 9, article id e0203430
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Bioprocess Technology
Research subject
Biochemical Process Engineering
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URN: urn:nbn:se:ltu:diva-70871DOI: 10.1371/journal.pone.0203430PubMedID: 30183773Scopus ID: 2-s2.0-85052855182OAI: oai:DiVA.org:ltu-70871DiVA, id: diva2:1248588
Note

Validerad;2018;Nivå 2;2018-09-17 (svasva)

Available from: 2018-09-17 Created: 2018-09-17 Last updated: 2019-03-26Bibliographically approved

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Muraleedharan, Madhu Nair

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