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Potential of aquatic oomycete as a novel feedstock for microbial oil grown on waste sugarcane bagasse
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering. Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology Roorkee (IIT-R), Roorkee, Uttarakhand, India .ORCID iD: 0000-0002-5285-1136
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.ORCID iD: 0000-0002-3687-6173
Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology Roorkee (IIT-R), Roorkee, Uttarakhand, India .
Molecular Microbiology Laboratory, Biotechnology Department, Indian Institute of Technology Roorkee (IIT-R), Roorkee, Uttarakhand, India .
2018 (English)In: Environmental science and pollution research international, ISSN 0944-1344, E-ISSN 1614-7499, Vol. 25, no 33, p. 33443-33454Article in journal (Refereed) Published
Abstract [en]

Biodiesel production from vegetable oils is not sustainable and economical due to the food crisis worldwide. The development of a cost-effective non-edible feedstock is essential. In this study, we proposed to use aquatic oomycetes for microbial oils, which are cellulolytic fungus-like filamentous eukaryotic microorganisms, commonly known as water molds. They differ from true fungi as cellulose is present in their cell wall and chitin is absent. They show parasitic as well as saprophytic nature and have great potential to utilize decaying animal and plant debris in freshwater habitats. To study the triacylglycerol (TAG) accumulation in the aquatic oomycetes, the isolated water mold Achlya diffusa was cultivated under semi-solid-state conditions on waste sugarcane bagasse, which was compared with the cultivation in Czapek (DOX) medium. A. diffusa grown on waste sugarcane bagasse showed large lipid droplets in its cellular compartment and synthesized 124.03 ± 1.93 mg/gds cell dry weight with 50.26 ± 1.76% w/w lipid content. The cell dry weight and lipid content of this water mold decreased to 89.54 ± 1.21 mg/gds and 38.82% w/w, respectively, when cultivated on standard medium Czapek-Dox agar (CDA). For the fatty acid profile of A. diffusa grown in sugarcane bagasse and CDA, in situ transesterification (IST) and indirect transesterification (IDT) approaches were evaluated. The lipid profile of this mold revealed the presence of C12:0, C14:0, C16:0, C18:0, C18:1, C18:2, C20:0, and C21:0 fatty acids, which is similar to vegetable oils. The biodiesel properties of the lipids obtained from A. diffusa satisfied the limits as determined by international standards ASTM-D6751 and EN-14214 demonstrating its suitability as a fuel for diesel engines.

Place, publisher, year, edition, pages
Springer, 2018. Vol. 25, no 33, p. 33443-33454
National Category
Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
URN: urn:nbn:se:ltu:diva-71042DOI: 10.1007/s11356-018-3183-8ISI: 000449920100053PubMedID: 30264348Scopus ID: 2-s2.0-85054156981OAI: oai:DiVA.org:ltu-71042DiVA, id: diva2:1252163
Note

Validerad;2018;Nivå 2;2018-11-21 (johcin)

Available from: 2018-10-01 Created: 2018-10-01 Last updated: 2018-12-03Bibliographically approved

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Patel, AlokMatsakas, Leonidas

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