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Simultaneous Quantification of L-Arginine and Monosaccharides during Fermentation: An Advanced Chromatography Approach
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.ORCID iD: 0000-0002-6337-6924
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.ORCID iD: 0000-0001-7500-2367
2019 (English)In: Molecules, ISSN 1420-3049, E-ISSN 1420-3049, Vol. 24, no 4, article id 802Article in journal (Refereed) Published
Abstract [en]

Increasing demand for L-arginine by the food and pharmaceutical industries has sparked the search for sustainable ways of producing it. Microbial fermentation offers a suitable alternative; however, monitoring of arginine production and carbon source uptake during fermentation, requires simple and reliable quantitative methods compatible with the fermentation medium. Two methods for the simultaneous quantification of arginine and glucose or xylose are described here: high-performance anion-exchange chromatography coupled to integrated pulsed amperometric detection (HPAEC-IPAD) and reversed-phase ultra-high-performance liquid chromatography combined with charged aerosol detection (RP-UHPLC-CAD). Both were thoroughly validated in a lysogeny broth, a minimal medium, and a complex medium containing corn steep liquor. HPAEC-IPAD displayed an excellent specificity, accuracy, and precision for arginine, glucose, and xylose in minimal medium and lysogeny broth, whereas specificity and accuracy for arginine were somewhat lower in medium containing corn steep liquor. RP-UHPLC-CAD exhibited high accuracy and precision, and enabled successful monitoring of arginine and glucose or xylose in all media. The present study describes the first successful application of the above chromatographic methods for the determination and monitoring of L-arginine amounts during its fermentative production by a genetically modified Escherichia coli strain cultivated in various growth media.

Place, publisher, year, edition, pages
MDPI, 2019. Vol. 24, no 4, article id 802
Keywords [en]
Charged aerosol detector, Fermentation, Integrated pulsed amperometric detection, Ion chromatography, L-arginine, Method validation, Monosaccharides, Reversed-phase liquid chromatography
National Category
Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
URN: urn:nbn:se:ltu:diva-73081DOI: 10.3390/molecules24040802ISI: 000460805900148PubMedID: 30813364Scopus ID: 2-s2.0-85062166570OAI: oai:DiVA.org:ltu-73081DiVA, id: diva2:1292921
Funder
Bio4EnergyThe Kempe Foundations
Note

Validerad;2019;Nivå 2;2019-08-20 (johcin)

Available from: 2019-03-01 Created: 2019-03-01 Last updated: 2019-08-20Bibliographically approved

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Ginesy, MireilleEnman, JosefineRusanova-Naydenova, DanielaRova, Ulrika

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