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Recovery of l-Arginine from Model Solutions and Fermentation Broth Using Zeolite-Y Adsorbent
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.
Swedish University of Agriculture Sciences.
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.ORCID iD: 0000-0001-9794-8305
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2019 (English)In: ACS Sustainable Chemistry & Engineering, ISSN 2168-0485, Vol. 7, no 9, p. 8900-8907Article in journal (Refereed) Published
Abstract [en]

Arginine was produced via fermentation of sugars using the engineered microorganism Escherichia coli. Zeolite-Y adsorbents in the form of powder and extrudates were used to recover arginine from both a real fermentation broth and aqueous model solutions. An adsorption isotherm was determined using model solutions and zeolite-Y powder. The saturation loading was determined to be 0.2 g/g using the Sips model. Arginine adsorbed from a real fermentation broth using either zeolite-Y powder or extrudates both showed a maximum loading of 0.15 g/g at pH 11. This adsorbed loading is very close to the corresponding value obtained from the model solution showing that under the experimental conditions the presence of additional components in the broth did not have a significant effect on the adsorption of arginine. Furthermore, a breakthrough curve was determined for extrudates using a 1 wt % arginine model solution. The selectivity for arginine over ammonia and alanine from the real fermentation broth at pH 11 was 1.9 and 8.3, respectively, for powder, and 1.0, and 4.1, respectively, for extrudates. To the best of our knowledge, this is the first time recovery of arginine from real fermentation broths using any type of adsorbent has been reported.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2019. Vol. 7, no 9, p. 8900-8907
Keywords [en]
Arginine, FAU, Fermentation broth, Escherichia coli, Adsorption, Selectivity, Breakthrough
National Category
Chemical Process Engineering Bioprocess Technology
Research subject
Chemical Technology; Biochemical Process Engineering
Identifiers
URN: urn:nbn:se:ltu:diva-73954DOI: 10.1021/acssuschemeng.9b00918ISI: 000467351200095Scopus ID: 2-s2.0-85065476739OAI: oai:DiVA.org:ltu-73954DiVA, id: diva2:1316015
Note

Validerad;2019;Nivå 2;2019-05-27 (oliekm)

Available from: 2019-05-15 Created: 2019-05-15 Last updated: 2019-08-19Bibliographically approved

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Faisal, AbrarGinesy, MireilleHolmgren, AllanEnman, JosefineHedlund, JonasGrahn, Mattias

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