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Single cell oil and ethanol production by the oleaginous yeast Trichosporon fermentans utilizing dried sweet sorghum stalks
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.ORCID iD: 0000-0002-7754-9398
Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, Athens, Greece.
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.ORCID iD: 0000-0002-3687-6173
2020 (English)In: Renewable energy, ISSN 0960-1481, E-ISSN 1879-0682, Vol. 146, p. 1609-1617Article in journal (Refereed) Published
Abstract [en]

The ability of the oleaginous yeast Trichosporon fermentans to efficiently produce lipids when cultivated in dried sweet sorghum was evaluated. First, lipid production was evaluated in synthetic media mimicking the composition of sweet sorghum stalks and optimized based on the nitrogen source and C: N ratio. Under optimum conditions, the lipid production reached 3.66 g/L with 21.91% w/w lipid content by using a mixture of sucrose, glucose and fructose and peptone at C: N ratio 160. Cultivation on pre-saccharified sweet sorghum stalks offered 1.97 g/L, while it was found that sweet sorghum stalks can support yeast growth and lipid production without the need for external nitrogen source addition. At an attempt to increase the carbon source concentration for optimizing lipid production, the Crabtree effect was observed in T. fermentans. To this end, the yeast was evaluated for its potential to produce ethanol under anaerobic conditions in synthetic media and sweet sorghum. The ethanol concentration at 100 g/L glucose was 40.31 g/L, while utilizing sweet sorghum by adding a distinct saccharification step and external nitrogen source offered ethanol concentration equal to 23.5 g/L. To the authors’ knowledge, this is the first time that the Crabtree effect is observed in T. fermentans.

Place, publisher, year, edition, pages
Elsevier, 2020. Vol. 146, p. 1609-1617
Keywords [en]
Trichosporon fermentans CBS 439.83, Crabtree effect, Ethanol, Sweet sorghum, Enzymatic saccharification, Microbial lipids
National Category
Chemical Engineering Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
URN: urn:nbn:se:ltu:diva-75377DOI: 10.1016/j.renene.2019.07.107Scopus ID: 2-s2.0-85069920649OAI: oai:DiVA.org:ltu-75377DiVA, id: diva2:1339672
Note

Validerad;2019;Nivå 2;2019-08-14 (johcin)

Available from: 2019-07-30 Created: 2019-07-30 Last updated: 2019-08-21Bibliographically approved

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Antonopoulou, IoMatsakas, Leonidas

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