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Tailoring Celluclast (R) Cocktail's Performance towards the Production of Prebiotic Cello-Oligosaccharides from Waste Forest Biomass
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.ORCID iD: 0000-0001-9164-7667
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.ORCID iD: 0000-0002-3687-6173
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.ORCID iD: 0000-0003-1336-2396
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2019 (English)In: Catalysts, E-ISSN 2073-4344, Vol. 9, no 11, article id 897Article in journal (Refereed) Published
Abstract [en]

The main objective of this study focused on the sustainable production of cellobiose and other cellulose-derived oligosaccharides from non-edible sources, more specifically, from forest residues. For this purpose, a fine-tuning of the performance of the commercially available enzyme mixture Celluclast® was conducted towards the optimization of cellobiose production. By enzyme reaction engineering (pH, multi-stage hydrolysis with buffer exchange, addition of β-glucosidase inhibitor), a cellobiose-rich product with a high cellobiose to glucose ratio (37.4) was achieved by utilizing organosolv-pretreated birch biomass. In this way, controlled enzymatic hydrolysis combined with efficient downstream processing, including product recovery and purification through ultrafiltration and nanofiltration, can potentially support the sustainable production of food-grade oligosaccharides from forest biomass. The potential of the hydrolysis product to support the growth of two Lactobacilli probiotic strains as a sole carbon source was also demonstrated

Place, publisher, year, edition, pages
MDPI, 2019. Vol. 9, no 11, article id 897
Keywords [en]
non-digestible oligosaccharides, Celluclast®, cellobiose, conduritol-B-epoxide, prebiotic, lignocellulose enzyme hydrolysis
National Category
Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
URN: urn:nbn:se:ltu:diva-77851DOI: 10.3390/catal9110897ISI: 000502278800023Scopus ID: 2-s2.0-85078137358OAI: oai:DiVA.org:ltu-77851DiVA, id: diva2:1396002
Note

Godkänd;2020;Nivå 0;2020-02-25 (johcin)

Available from: 2020-02-25 Created: 2020-02-25 Last updated: 2023-09-05Bibliographically approved
In thesis
1. Lytic Polysaccharide MonoOxygenases; their role for lignocellulose depolymerization and production of (functional) biobased compounds
Open this publication in new window or tab >>Lytic Polysaccharide MonoOxygenases; their role for lignocellulose depolymerization and production of (functional) biobased compounds
2021 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Increased environmental concerns over petroleum-based products triggered the quest to find a sustainable alternative for fuels, chemicals etc. Lignocellulose biomass, due to its abundance, is considered as one of the most promising sustainable sources for the production of fuels and chemicals, while replacing the traditional petroleum resources. In a biorefinery, by choosing a greener biochemical conversion process with cellulolytic enzymes, cellulose from biomass is depolymerized into monomeric sugars and residual fibers; which can be later converted into a spectra of value added products.

Lytic polysaccharide monooxygenases (LPMOs) are one of the essential groups of enzymes in the bioconversion of lignocellulose. They are copper active enzymes that are produced by different polysaccharide degrading organisms in nature, such as lignocellulolytic fungi. In lignocellulose degradation, they are different from the traditional hydrolytic cellulolytic enzymes with their unique way of oxidative breakage of cellulose, in the presence of a co-substrate such as oxygen, and a reducing agent like lignin in the biomass. Their ability to enhance the action of traditional cellulases in cellulose depolymerization make them an integral part of today’s commercial cellulosic cocktails.

Primary goals of biorefinery research include efficient liquefaction of lignocellulose in order to increase the release of monomeric sugars towards the production of various chemicals and fuels, together with the potential use of residual fibers for the production of value-added products; all by minimizing the release of undesired by-products and the environmental impact of the process. LPMOs, along with other cellulases, have been shown to be very much beneficial in this.

This thesis comprises the study of LPMOs from different fungal origin, in their depolymerization ability on various substrates, including both model substrates and natural biomass samples. The evaluation was done based on their ability to release neutral and oxidized sugars, as well as their capability to promote liquefaction. Effect of various pretreatment methods of lignocellulose on the action of LPMOs was studied, together with their capability to use lignin present in the wood as a reducing agent, which gives a better understanding about their function in nature. Lastly, their role in producing value added materials such as nanocellulose, the prebiotic disaccharide cellobiose, from lignocellulose was also evaluated. 

Place, publisher, year, edition, pages
Luleå University of Technology, 2021
Series
Doctoral thesis / Luleå University of Technology 1 jan 1997 → …, ISSN 1402-1544
National Category
Industrial Biotechnology Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
urn:nbn:se:ltu:diva-82232 (URN)978-91-7790-745-9 (ISBN)978-91-7790-746-6 (ISBN)
Public defence
2021-02-12, A109, 10:00 (English)
Opponent
Supervisors
Funder
Swedish Energy Agency
Available from: 2021-01-11 Created: 2021-01-10 Last updated: 2023-09-05Bibliographically approved

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Karnaouri, Anthi C.Matsakas, LeonidasMuraleedharan, Madhu NairChristakopoulos, PaulRova, Ulrika

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