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Alkaline and Alkaline-Oxidative Pretreatment and Hydrolysis of Herbaceous Biomass for Growth of Oleaginous Microbes
Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, USA.
Department of Agricultural and Biological Engineering, Michigan State University, East Lansing, USA.
Element Materials Technology, Plymouth, USA.
Department of Chemical and Biological Engineering, University of Wisconsin, Madison, USA.
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2019 (English)In: Microbial Lipid Production: Methods and Protocols / [ed] Venkatesh Balan, Springer Nature, 2019, p. 173-182Chapter in book (Other academic)
Abstract [en]

This chapter describes methods for generation of hydrolysates amenable to conversion to microbial lipids from herbaceous lignocellulosic biomass utilizing either mild alkali pretreatment with NaOH or alkaline hydrogen peroxide pretreatment with NaOH and H2O2. This pretreatment is followed by enzymatic hydrolysis of the plant cell wall polysaccharides to yield hydrolysates. These hydrolysates are composed primarily of the monosaccharides glucose and xylose as well as acetate and phenolic monomers that may all serve as a source of renewable carbon to produce microbial lipids. Application of these mild pretreatment conditions minimizes the generation of inhibitors, enabling microbial cultivations to often be performed without the need for detoxification.

Place, publisher, year, edition, pages
Springer Nature, 2019. p. 173-182
Series
Methods in Molecular Biology, ISSN 1064-3745, E-ISSN 1940-6029
Keywords [en]
Alkaline pretreatment, Plant cell wall deconstruction, Cellulosic sugars, Alkaline hydrogen peroxide pretreatment
National Category
Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
URN: urn:nbn:se:ltu:diva-78658DOI: 10.1007/978-1-4939-9484-7_11PubMedID: 31148129Scopus ID: 2-s2.0-85066808838OAI: oai:DiVA.org:ltu-78658DiVA, id: diva2:1426300
Note

ISBN för värdpublikation: 978-1-4939-9483-0, 978-1-4939-9484-7

Available from: 2020-04-24 Created: 2020-04-24 Last updated: 2020-04-24Bibliographically approved

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Hodge, David B.

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