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The crystal structure of a Fusarium oxysporum feruloyl esterase that belongs to the tannase family
Institute of Chemical Biology, National Hellenic Research Foundation, Athens, Greece. School of Chemical Engineering, National Technical University of Athens, Greece.
School of Chemical Engineering, National Technical University of Athens, Greece.
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.ORCID iD: 0000-0003-0079-5950
Institute of Chemical Biology, National Hellenic Research Foundation, Athens, Greece.
2020 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468Article in journal (Refereed) Epub ahead of print
Abstract [en]

Feruloyl esterases are enzymes of industrial interest that catalyse the hydrolysis of the ester bond between hydroxycinnamic acids such as ferulic acid and sugars present in the plant cell wall. Although there are several structures of biochemically characterized feruloyl esterases available, the structural determinants of their substrate specificity are not yet fully understood. Here, we present the crystal structure of a feruloyl esterase from Fusarium oxysporum (FoFaeC) at 2.3 Å resolution. Similar to the two other tannase‐like feruloyl esterases, FoFaeC features a large lid domain covering the active site with potential regulatory role and a disulphide bond that brings together the serine and histidine of the catalytic triad. Differences are mainly observed in the metal coordination site and the substrate binding pocket.

Place, publisher, year, edition, pages
John Wiley & Sons, 2020.
Keywords [en]
biocatalysis, feruloyl esterase, Fusarium oxysporum, tannase family, X‐ray crystallography
National Category
Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
URN: urn:nbn:se:ltu:diva-78708DOI: 10.1002/1873-3468.13776ISI: 000526163500001PubMedID: 32297315Scopus ID: 2-s2.0-85083526781OAI: oai:DiVA.org:ltu-78708DiVA, id: diva2:1426999
Available from: 2020-04-28 Created: 2020-04-28 Last updated: 2020-04-28

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Christakopoulos, Paul

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