Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Potential effects of long term abuse of anabolic androgen steroids on human skeletal muscle
Sports Medicine Unit, Department of Community Medicine and Rehabilitation, Umeå University, Umeå, Sweden.
Sports Medicine Unit, Department of Community Medicine and Rehabilitation, Umeå University, Umeå, Sweden.
Sports Medicine Unit, Department of Community Medicine and Rehabilitation, Umeå University, Umeå, Sweden.
Luleå University of Technology, Department of Health Sciences, Health, Medicine and Rehabilitation.ORCID iD: 0000-0003-3628-0705
Show others and affiliations
2020 (English)In: Journal of Sports Medicine and Physical Fitness, ISSN 0022-4707, E-ISSN 1827-1928, Vol. 60, no 7, p. 1040-1048Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: We have previously evaluated muscle functions and morphology in power athletes of long term (5 to15 years) abuse of anabolic androgen steroids (AAS; Doped) and in clean power athletes (Clean), and observed significant improvements in both muscle morphology and muscle functions in Doped. To our knowledge, the effects of long term AAS abuse on human muscle protein profile have never been studied.

METHODS: The study examined further the muscle biopsies using a two-dimensional difference gel electrophoresis (2D DIGE) for proteomic screening and protein expression. Cellular localization/distribution of specific proteins identified by proteomic analysis was examined using immunohistochemistry (IHC).

RESULTS: Different protein profiles were observed between Doped and Clean, and a valid orthogonal projection of latent structure discriminant analysis model was built (N = 16, X = 5, R2 = 0.88/Q2 = 0.84, p = 0.0005), which separated Doped from Clean. Liquid chromatography followed by tandem spectrometry identified 14 protein spots (representing nine different proteins) of significant difference in relative quantity (p < 0.05), of which nine spots were down-regulated in Doped compared with Clean. IHC revealed no significant alteration in cellular localization in phosphoglucomutase-1 and heat shock protein beta-1, but indeed in two reference proteins desmin and F-actin in Doped.

CONCLUSIONS: Long term abuse of AAS in combination with training is potentially associated with alterations in skeletal muscle protein profile and protein expression, and structural proteins rather than non-structural proteins are preferentially affected in cellular localization/distribution.

Place, publisher, year, edition, pages
Edizioni Minerva Medica , 2020. Vol. 60, no 7, p. 1040-1048
Keywords [en]
Doping in sports, Testosterone congeners, Skeletal muscle, Proteomics, Gene expression
National Category
Nursing Other Medical Engineering
Research subject
Nursing; Medical Science
Identifiers
URN: urn:nbn:se:ltu:diva-78781DOI: 10.23736/S0022-4707.20.10443-2ISI: 000545319500013PubMedID: 32343079Scopus ID: 2-s2.0-85087433642OAI: oai:DiVA.org:ltu-78781DiVA, id: diva2:1428295
Note

Validerad;2020;Nivå 2;2020-07-14 (alebob)

Available from: 2020-05-05 Created: 2020-05-05 Last updated: 2021-05-04Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records

Tegner, YelvertonEriksson, Anders

Search in DiVA

By author/editor
Tegner, YelvertonEriksson, Anders
By organisation
Health, Medicine and RehabilitationNursing and Medical technology
In the same journal
Journal of Sports Medicine and Physical Fitness
NursingOther Medical Engineering

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 270 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf