The present study was designed to assess the psycho-physiological responses of physically untrained individuals to mobile-based multi-stimulating, circuit-like, multiplejoint conditioning (CircuitHiu) performed either once (IxCircuitmu) or twice (2xCircuitHiu) daily for 4 weeks. In this single-center, two-arm randomized, controlled study, 24 men and women (age: 25 +/- 5 years) first received no training instructions for 4 weeks and then performed 4 weeks of either IxCircuitnirr or 2xCircuitHiu (5 men and 7 women in each group) daily. The IxCircuitnirr and 2xCircuitHiu participants carried out 90.7 and 85.7% of all planned training sessions, respectively, with average heart rates during the 6-min sessions of 74.3 and 70.8% of maximal heart rate. Body, fat and fat-free mass, and metabolic rate at rest did not differ between the groups or between time-points of measurement. Heart rate while running at 6 km h(-1) declined after the intervention in both groups. Submaximal and peak oxygen uptake, the respiratory exchange ratio and heart rate recovery were not altered by either intervention. The maximal numbers of push-ups, leg-levers, burpees, 45 degrees-one-legged squats and 30-s skipping, as well as perception of general health improved in both groups. Our IxCircuitHiu or 2xCircuitHiiT interventions improved certain parameters of functional strength and certain dimensions of quality of life in young untrained individuals. However, they were not sufficient to enhance cardio-respiratory fitness, in particular peak oxygen uptake.