A label-free graphene-based impedimetric biosensor for real-time tracing of the cytokine storm in blood serum; suitable for screening COVID-19 patientsNano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, University of Tehran, P. O. Box 14395/515, Tehran, Iran;Nano Electronic Center of Excellence, Thin Film and Nano Electronics Lab, School of Electrical and Computer Engineering, University of Tehran, P. O. Box 14395/515, Tehran, Iran;School of Biology, College of Science, University of Tehran, P. O. Box: 14155-6655, Tehran, Iran.
Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, University of Tehran, P. O. Box 14395/515, Tehran, Iran;Nano Electronic Center of Excellence, Thin Film and Nano Electronics Lab, School of Electrical and Computer Engineering, University of Tehran, P. O. Box 14395/515, Tehran, Iran;Integrative Oncology Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran.
Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, University of Tehran, P. O. Box 14395/515, Tehran, Iran;Nano Electronic Center of Excellence, Thin Film and Nano Electronics Lab, School of Electrical and Computer Engineering, University of Tehran, P. O. Box 14395/515, Tehran, Iran.
Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, University of Tehran, P. O. Box 14395/515, Tehran, Iran;Nano Electronic Center of Excellence, Thin Film and Nano Electronics Lab, School of Electrical and Computer Engineering, University of Tehran, P. O. Box 14395/515, Tehran, Iran.
Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, University of Tehran, P. O. Box 14395/515, Tehran, Iran;Nano Electronic Center of Excellence, Thin Film and Nano Electronics Lab, School of Electrical and Computer Engineering, University of Tehran, P. O. Box 14395/515, Tehran, Iran.
SEPAS Pathology Lab, P. O. Box 1991945391, Tehran, Iran.
Imam Hossein Clinical Research Development Center, Imam Hossein Hospital, Shahid Beheshti University of Medical Science, Tehran, Iran.
ATMP Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, P. O. Box 15179/64311, Tehran, Iran.
Nano Electronic Center of Excellence, Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, University of Tehran, P. O. Box 14395/515, Tehran, Iran;Nano Electronic Center of Excellence, Thin Film and Nano Electronics Lab, School of Electrical and Computer Engineering, University of Tehran, P. O. Box 14395/515, Tehran, Iran;Cancer Institute, Imam-Khomeini Hospital, Tehran University of Medical Sciences, P. O. Box 13145-158, Tehran, Iran;UT&TUMS Cancer electronic Research Center, Tehran University of Medical Sciences, Tehran, Iran.
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2021 (English)In: RSC Advances, E-ISSN 2046-2069, Vol. 11, no 55, p. 34503-34515Article in journal (Refereed) Published
Abstract [en]
Concurrent with the pandemic announcement of SARS-CoV-2 infection by the WHO, a variety of reports were published confirming the cytokine storm as the most mortal effect of the virus on the infected patients. Hence, cytokine storm as an evidenced consequence in most of the COVID-19 patients could offer a promising opportunity to use blood as a disease progression marker. Here, we have developed a rapid electrochemical impedance spectroscopy (EIS) sensor for quantifying the overall immune activity of the patients. Since during the cytokine storm many types of cytokines are elevated in the blood, there is no need for specific detection of a single type of cytokine and the collective behavior is just measured without any electrode functionalization. The sensor includes a monolayer graphene on a copper substrate as the working electrode (WE) which is able to distinguish between the early and severe stage of the infected patients. The charge transfer resistance (RCT) in the moderate and severe cases varies about 65% and 138% compared to the normal groups, respectively and a specificity of 77% and sensitivity of 100% based on ELISA results were achieved. The outcomes demonstrate a significant correlation between the total mass of the three main hypercytokinemia associated cytokines including IL-6, TNF-α and IFN-γ in patients and the RCT values. As an extra application, the biosensor's capability for diagnosis of COVID-19 patients was tested and a sensitivity of 92% and specificity of 50% were obtained compared to the RT-PCR results.
Place, publisher, year, edition, pages
Royal Society of Chemistry, 2021. Vol. 11, no 55, p. 34503-34515
Keywords [en]
General Chemical Engineering, General Chemistry
National Category
Nano Technology Infectious Medicine
Research subject
Machine Elements
Identifiers
URN: urn:nbn:se:ltu:diva-87708DOI: 10.1039/d1ra04298jISI: 000710480500001PubMedID: 35494759Scopus ID: 2-s2.0-85120046571OAI: oai:DiVA.org:ltu-87708DiVA, id: diva2:1607474
Note
Validerad;2021;Nivå 2;2021-11-04 (johcin)
2021-11-012021-11-012022-09-15Bibliographically approved