Enzymatic CO₂ conversion offers a promising strategy for alleviating global warming and promoting renewable energy exploitation, while the high cost of cofactors is a bottleneck for large-scale applications. To address the challenge, cofactor regeneration is usually coupled with the enzymatic reaction. Meanwhile, artificial cofactors have been developed to further improve conversion efficiency and decrease cost. In this review, the methods, such as enzymatic, chemical, electrochemical, and photochemical catalysis, developed for cofactor regeneration, together with those developed artificial cofactors, were summarized and compared to offer a solution for large-scale enzymatic CO₂ conversion in a sustainable way.