Background: Maximal force-velocity (F/v) profiles for track cyclists are commonly derived from ergometer sprints using an isovelocity or isoinertial approach. Previously, an attempt was made to derive maximal F/v profiles from a single maximal 65-m sprint on the cycling track. Hypothesising that this approach may not accurately reflect the fatigue-free F/v profile, we propose an alternative procedure and compare it to the previous method. Moreover, we test for the impact of gear ratio on diagnostic results.

Methods: Twelve elite track cyclists completed a high-cadence low-resistance pedalling test on a freestanding roller (motoric test) and two series of three maximal 65-m sprints on a cycling track with different gear ratios. F/v profiles were calculated based on the measured crank force and cadence either during the first 6–7 revolutions (≤6 s) on the track (model I) or were derived from the first 3–4 revolutions (≤3 s) on the track combined with 1 or 2 fatigue-free cycles at cadences above 160 rpm from the motoric test (model II).

Results: Although both models exhibit high-to-excellent linearity between force and velocity, the extrapolated isometric force was higher (1507.51 ± 257.60 N and 1384.35 ± 276.84 N; p < 0.002; d = 2.555) and the slope steeper (−6.78 ± 1.17 and −5.24 ± 1.11; p < 0.003, d = −2.401) with model I. An ICC of 1.00 indicates excellent model consistency when comparing the F/v profiles (model II) derived from the different geared sprints.

Conclusions: Assuring fatigue-free measurements and including high-cadence data points in the calculations provide valid maximal F/v and P/v profiles from a single acceleration-sprint independent of gear ratio.