The backbone of European infrastructure was built after the end of the second World War and has reached, or is near to, the end of its nominal design life. This issue urges the development of structural assessment procedures that can provide infrastructure managers the information to make decisions for repairing, upgrading, or replacement. In this paper, a methodology based on load testing and Structural Health Monitoring (SHM) for the assessment of a 65- year-old prestressed concrete bridge located in Northern Sweden is presented. The retrieved data is used to develop and calibrate structural models with different levels of data completeness. The SHM procedure includes the evaluation of material properties by diagnostics, definition of the layout and installation of the instrumentation, test execution, and data analysis. A preliminary structural model is developed based only on the original design parameters, and it is sequentially updated with monitoring data retrieved during a performed proof loading test of the bridge.