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Expression, characterization and structural modelling of a feruloyl esterase from the thermophilic fungus Myceliophthora thermophila
School of Chemical Engineering, National Technical University of Athens.
School of Chemical Engineering, National Technical University of Athens.
School of Chemical Engineering, National Technical University of Athens.
2012 (English)In: Applied Microbiology and Biotechnology, ISSN 0175-7598, E-ISSN 1432-0614, Vol. 94, no 2, p. 399-411Article in journal (Refereed) Published
Abstract [en]

A ferulic acid esterase (FAE) from the thermophilic fungus Myceliophthora thermophila (synonym Sporotrichum thermophile), belonging to the carbohydrate esterase family 1 (CE-1), was functionally expressed in methylotrophic yeast Pichia pastoris. The putative FAE from the genomic DNA was successfully cloned in P. pastoris X-33 to confirm that the enzyme exhibits FAE activity. The recombinant FAE was purified to its homogeneity (39 kDa) and subsequently characterized using a series of model substrates including methyl esters of hydroxycinnamates, alkyl ferulates and monoferuloylated 4-nitrophenyl glycosides. The substrate specificity profiling reveals that the enzyme shows a preference for the hydrolysis of methyl caffeate and p-coumarate and a strong preference for the hydrolysis of n-butyl and iso-butyl ferulate. The enzyme was active on substrates containing ferulic acid ester linked to the C-5 and C-2 linkages of arabinofuranose, whilst it was found capable of de-esterifying acetylated glucuronoxylans. Ferulic acid (FA) was efficiently released from destarched wheat bran when the esterase was incubated together with an M3 xylanase from Trichoderma longibrachiatum (a maximum of 41% total FA released after 1 h incubation). Prediction of the secondary structure of MtFae1a was performed in the PSIPRED server whilst modelling the 3D structure was accomplished by the use of the HH 3D structure prediction server.

Place, publisher, year, edition, pages
2012. Vol. 94, no 2, p. 399-411
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Bioprocess Technology
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URN: urn:nbn:se:ltu:diva-2777DOI: 10.1007/s00253-011-3612-9ISI: 000302035500010PubMedID: 22012339Scopus ID: 2-s2.0-84862651303Local ID: 076940f4-63a1-400b-8023-d8919c9ee03eOAI: oai:DiVA.org:ltu-2777DiVA, id: diva2:975630
Note
Uppr├Ąttat; 2012; 20130212 (ysko)Available from: 2016-09-29 Created: 2016-09-29 Last updated: 2023-09-05Bibliographically approved

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