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Use of dried sweet sorghum for the efficient production of lipids by the yeast Lipomyces starkeyi CBS 1807
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.ORCID iD: 0000-0002-3687-6173
Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens.
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.ORCID iD: 0000-0001-7500-2367
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.ORCID iD: 0000-0003-0079-5950
2014 (English)In: Industrial crops and products (Print), ISSN 0926-6690, E-ISSN 1872-633X, Vol. 62, p. 367-372Article in journal (Refereed) Published
Abstract [en]

The ability of the oleaginous yeast Lipomyces starkeyi to efficiently produce lipids when cultivated on saccharified sweet sorghum stalks juice was evaluated. Initially the production of lipids using synthetic media mimicking sweet sorghum stalks has been studied and optimized concerning the nitrogen source and the C:N ratio. Under optimum conditions (yeast extract as nitrogen source and C:N ratio of 190) the lipid production reached 5.81 g/L with a lipid content of 47.3% (w/w) from a mixture of sucrose, glucose and fructose, mimicking the sugar composition of sorghum. When cultivated on sweet sorghum stalks juice, it was observed that no external nitrogen addition was necessary which could result in substantial decrease of the initial C:N ratio. Moreover a distinct saccharification process prior to yeast cultivation improved the lipid production yield as it resulted in an increase of the C:N ratio. The highest lipid production, which was 6.40 g/L with a lipid content of 29.5% (w/w), was obtained when juice from saccharified sweet sorghum stalks at an initial sorghum content of 12% (w/w) was used as feedstock.

Place, publisher, year, edition, pages
2014. Vol. 62, p. 367-372
National Category
Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
URN: urn:nbn:se:ltu:diva-4255DOI: 10.1016/j.indcrop.2014.09.011ISI: 000345468800049Scopus ID: 2-s2.0-84907462633Local ID: 22c727a2-36fe-4361-81bc-07cd76fe7fb0OAI: oai:DiVA.org:ltu-4255DiVA, id: diva2:977119
Note
Validerad; 2014; 20140906 (pauchr)Available from: 2016-09-29 Created: 2016-09-29 Last updated: 2018-07-10Bibliographically approved

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Matsakas, LeonidasRova, UlrikaChristakopoulos, Paul

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