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Characterization and application of a novel class II thermophilic peroxidase from Myceliophthora thermophila in biosynthesis of polycatechol
Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens.ORCID iD: 0000-0003-0361-7690
Luleå University of Technology, Department of Civil, Environmental and Natural Resources Engineering, Chemical Engineering.ORCID iD: 0000-0003-0079-5950
Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens.
2015 (English)In: Enzyme and microbial technology, ISSN 0141-0229, E-ISSN 1879-0909, Vol. 75-76, p. 49-56Article in journal (Refereed) Published
Abstract [en]

A peroxidase from the thermophilic fungus Myceliophthora thermophila that belongs to ascomycete Class II based on PeroxiBase classification was functionally expressed in methylotrophic yeast Pichia pastoris. The putative peroxidase from the genomic DNA was successfully cloned in P. pastoris X-33 under the transcriptional control of the alcohol oxidase (AOX1) promoter. The heterologous production was greatly enhanced by the addition of hemin with a titer of 0.41 U mL−1 peroxidase activity at the second day of incubation. The recombinant enzyme was purified to homogeneity (50 kDa) and characterized using a series of phenolic substrates that indicated similar characteristics with those of generic peroxidases. In addition, the enzyme was found thermostable, retaining its activity for temperatures up to 60 oC after eight hours incubation. Moreover, the enzyme displayed remarkable H2O2 stability, retaining more than 80% of its initial activity after 24 hours incubation in 5000- fold molar excess of H2O2. The ability of the peroxidase to polymerize catechol at high superoxide concentrations, together with its high thermostability and substrate specificity, indicate a potential commercial significance of the enzyme.

Place, publisher, year, edition, pages
2015. Vol. 75-76, p. 49-56
National Category
Bioprocess Technology
Research subject
Biochemical Process Engineering
Identifiers
URN: urn:nbn:se:ltu:diva-4301DOI: 10.1016/j.enzmictec.2015.04.012Local ID: 23c0db13-e657-42c1-a95a-225a301b7b29OAI: oai:DiVA.org:ltu-4301DiVA: diva2:977165
Note

Validerad; 2015; Nivå 2; 20150511 (andbra)

Available from: 2016-09-29 Created: 2016-09-29 Last updated: 2017-11-24Bibliographically approved

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