Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Determination of citalopram enantiomers in human plasma by liquid chromatographic separation on a Chiral-AGP column
Analytical Pharmaceutical Chemistry, Uppsala University, Biomedical Centre, Uppsala, Sweden.
1996 (English)In: Journal of Chromatography. Biomedical Applications, ISSN 0378-4347, Vol. 685, no 2, p. 299-305Article in journal (Refereed) Published
Abstract [en]

A liquid chromatographic method for the quantitative analysis of S-(+)- and R-(-)-citalopram in human plasma has been developed and validated. The enantiomers of citalopram and the internal standard, R-(+)-propranolol, were extracted from alkaline plasma with 2% n-butanol in n-hexane. After a clean-up step, the organic phase was evaporated and the residues dissolved in 50-100 microliters of 0.001 M HCl. The separation was performed on a Chiral-AGP column with 3.0 mM N-dodecyl-N,N-dimethylammonio-3-propanesulfonate and 10 mM hexanoic acid in phosphate buffer pH 6.5 as the mobile phase. The limit of detection was estimated to be 1 ng/ml (S/N approximately equal to 3) for each enantiomer monitoring UV absorption at 240 nm. In the range studied, 2.31-191 ng/ml, the recoveries were quantitative and the coefficients of variations were between 2.47% and 11.5%.

Place, publisher, year, edition, pages
1996. Vol. 685, no 2, p. 299-305
Identifiers
URN: urn:nbn:se:ltu:diva-4385DOI: 10.1016/S0378-4347(96)00177-6Local ID: 25392fa0-e299-11db-b078-000ea68e967bOAI: oai:DiVA.org:ltu-4385DiVA, id: diva2:977251
Note

Godkänd; 1996; 20070404 (andbra)

Available from: 2016-09-29 Created: 2016-09-29 Last updated: 2018-01-12Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full text

Authority records BETA

Haupt, Dan

Search in DiVA

By author/editor
Haupt, Dan

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 41 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf