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Studies on a N-acetyl-β-d-glucosaminidase produced by Fusarium oxysporum F3 grown in solid-state fermentation
National Technical University of Athens.
National Technical University of Athens.
National Technical University of Athens.
National Technical University of Athens.
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2004 (English)In: Process Biochemistry, ISSN 1359-5113, E-ISSN 1873-3298, Vol. 39, no 11, p. 1599-1605Article in journal (Refereed) Published
Abstract [en]

Fusarium oxysporum F3 produced N-acetyl-β-d-glucosaminidase when grown on wheat bran and chitin as carbon sources in solid-state fermentation. The initial moisture content and pH of growth medium were 65% and 6.0, respectively, and the enzyme yield 23.6 U g−1 carbon source. Two isozymes of N-acetyl-β-d-glucosaminidase, called N-acetyl-β-d-glucosaminidases I and II, were isolated from the culture filtrate of F. oxysporum F3. The filtrate was subjected to ammonium sulphate fractionation followed by anion exchange, gel filtration, hydrophobic interaction and cation exchange chromatography. The optimum pH of isozymes I and II was 5.0 and 6.0, respectively, whereas maximum activity of both isozymes was obtained at 40 °C. The Km of isozymes I and II was 49.6 and 48.6 μM and the Vmax 1.24 and 0.26 μmol mg−1 min−1, respectively, on p-nitrophenyl N-acetyl-β-d-glucosaminide as substrate. The molecular mass of isozymes I and II was calculated to be 67 kDa by SDS–PAGE.

Place, publisher, year, edition, pages
2004. Vol. 39, no 11, p. 1599-1605
Identifiers
URN: urn:nbn:se:ltu:diva-5519DOI: 10.1016/S0032-9592(03)00287-5Local ID: 3a4421cc-5f55-4300-a540-95b66975b97eOAI: oai:DiVA.org:ltu-5519DiVA: diva2:978393
Note
Upprättat; 2004; 20130215 (ysko)Available from: 2016-09-29 Created: 2016-09-29 Last updated: 2017-11-24Bibliographically approved

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