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Optimization of xylanase production by Thermomyces lanuginosus in tomato seed meal using response surface methodology
National Technical University of Athens.
National Technical University of Athens.
2006 (English)In: World Journal of Microbiology & Biotechnology, ISSN 0959-3993, E-ISSN 1573-0972, Vol. 22, no 5, p. 501-506Article in journal (Refereed) Published
Abstract [en]

A 32 central composite experimental design was performed with the aim of optimizing xylanase production by Thermomyces lanuginosus grown on corn cobs in submerged cultures. Xylanase production was first tested on different nitrogen sources (tomato skin, tomato seed meal, corn steep liquor, meat peptone, bacto-tryptone and yeast extract). Tomato seed meal was the selected substrate to test the effect of two variables on xylanase production (corn cobs and tomato seed meal concentrations). A second-order quadratic model and a response surface method showed that the optimum condition for xylanase production was corn cobs 4.6% (w/v) and tomato seed meal 2.1% (w/v). The optimum conditions found were transferred to 7-l bioreactors, where activities as high as 1630 U/ml were obtained.

Place, publisher, year, edition, pages
2006. Vol. 22, no 5, p. 501-506
Identifiers
URN: urn:nbn:se:ltu:diva-6232DOI: 10.1007/s11274-005-9063-7Local ID: 46e89201-c6a4-4112-8c4e-25c52ae80c7eOAI: oai:DiVA.org:ltu-6232DiVA, id: diva2:979109
Note
Upprättat; 2006; 20130214 (ysko)Available from: 2016-09-29 Created: 2016-09-29 Last updated: 2017-11-24Bibliographically approved

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Christakopoulos, Paul

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